동의신경정신과학회지 (Journal of Oriental Neuropsychiatry)

  • 제21권1호
  • /
  • Pages.1-11
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  • 2010
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  • 1226-6396(pISSN)
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  • 2234-4942(eISSN)
대한한방신경정신과학회 (The Korean Society of Oriental Neuropsychiatry)
권호 표지

가미귀비탕(加味歸脾湯)이 P815세포의 serotonin 대사 과정에 미치는 영향

Study on Effect to Serotonin Metabolism of Gamiguibi-tang on P815 Cell

  • 노동진 (대전대학교 한의과대학 신경정신과교실) ;
  • 정인철 (대전대학교 한의과대학 신경정신과교실)
  • No, Dong-Jin (Dept. of Oriental Neuropsychiatry College of Oriental Medicine, Dae-Jeon University) ;
  • Jung, In-Chul (Dept. of Oriental Neuropsychiatry College of Oriental Medicine, Dae-Jeon University)
  • 투고 : 2010.02.06
  • 심사 : 2010.03.20
  • 발행 : 2010.03.30
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초록

Objectives: This study was perfomed to investigate the antioxidant activity and serotonin activity of Gami-guibi-tang on P815 Mast Cell. Methods: The effects of Gami-guibi-tang on activation of TPH-1 mRNA and AAADC mRNA in P815 mast cell were investigated. The effect of Gami-guibi-tang on content of serotonin in P815 mast cell was investigated. The effects of Gami-guibi-tang on activation of DPPH radical scavenging and SOD in P815 mast cell were investigated. Results: 1. The Gami-guibi-tang increased SOD activity and DPPH radical scavenging activity. 2. The Gami-guibi-tang increased the intracellular concentration of serotoninin 60 ${\mu}g/ml$, 80 ${\mu}g/ml$ experiment group. 3. The Gami-guibi-tang increased menaingful the manifestation TPH mRNA. 4. The manifestation of AAADC and MAO mRNA have not made menaingful changes on Gami-guibi-tang. Conclusions: This experiment shows that Gami-guibi-tang had significant anti-oxidative effect. And Gami-guibi-tang increased the intracellular concentration of serotonin. Therefore, Gami-guibi-tang can be used by the medication of major depression disorder. But Study on mechanism of increased serotonin and clinical research of Gami-guibi-tang is suggested for future research.

키워드

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