The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
권호 표지

The Antiallergic Effects of Yeotaectonggi-tang (YTT) on the Mice Model with Allergic Rhinitis by Regulation of PPAR-$\gamma$

PPAR-$\gamma$ 조절을 통한 여택통기탕(麗澤通氣湯)의 항알레르기와 항염증 효과

  • 손정민 (동국대학교 한의과대학 한방안이비인후피부과학교실) ;
  • 홍승욱 (동국대학교 한의과대학 한방안이비인후피부과학교실)
  • Received : 2010.07.07
  • Accepted : 2010.08.02
  • Published : 2010.08.25
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Abstract

Objectives : The aim of this study is to investigate the antiallergic effects of YTT on allergic rhinitis by regulation of peroxisome proliferator-activated receptor gamma (PPAR-$\gamma$). Methods : The thirty rats were divided into three groups : normal group, control (allergic rhinitis elicited) group, sample (Yeotaectonggi-tang treated after allergic rhinitis elicitation) group. To induce allergic rhinitis in control group and sample group, rats were sensitized intraperitoneally with 0.1% ovalbumin(OVA) solution 3 times at intervals of 1 week. Then intranasal sensitization was performed by diffusing 0.1% ovalbumin(OVA) solution 3 times at intervals of 2 days. After that time rats in sample group were oral administration treated by YTT for 7days. The change of the amounts of eosinophil, substance P, MIP-2, PPAR-$\gamma$, IL-4, iNOS were observed in each group. we used the statistical method of ANOVA test(p<0.05). Results : The number of eosinophil in sample group noticeably decreased than control group. And the decrease of substance P and MIP-2 positive reaction were observed in mucosa. YTT inhibited IL-4 and iNOS production, mucus secretion, activation of mast cells and fibrosis remodeling by regulation of PPAR-$\gamma$ activation. Conclusion : According to the above results, it is considered that YTT mitigated mucosa damage on the mice model with allergic rhinitis by regulation of PPAR-$\gamma$.

Keywords

References

  1. Divya Seth, Elizabeth Secord, and Deepak Kamat. Allergic Rhinitis. Clinical Pediatrics, Jun 2007;46:401-7. https://doi.org/10.1177/0009922806298703
  2. Strachan D, Sibbald B,Weiland S, Ait-Khaled N, Anabwani G, Anderson HR, et al. Worldwide variations in prevalence of symptoms of allergic rhinoconjunctivitis in children:the international Study of Asthma and Allergies in Childhood (ISAAC). Pediatr Allergy Immunol 1997;8(4):161-76. https://doi.org/10.1111/j.1399-3038.1997.tb00156.x
  3. 지혜미, 김경원, 김창수, 손명현, 신동천, 김규언. 2006년 초등학생의 알레르기 질환 유병률의 전국적 역학조사. 소아알레르기 호흡기. 2009;19(2):165-72.
  4. 白萬基 編著. 最新耳鼻咽喉科學. 一潮閣. 2001:169-72.
  5. 李東垣. 東垣十種醫書. 서울:一中社. 1993:179-80.
  6. 許俊. 對譯東醫寶鑑. 서울:法仁文化社. 1999:592.
  7. 손동석, 이태헌, 김윤범. 비질환의 치료방제에 대한 문헌적 고찰. 대한외관과학회지. 2001;14(1):11-31.
  8. 윤찬호, 노석선. 비색의 병인.병기.치료에 대한 문헌적 고찰. 대한외관과학회지. 1999;12(1):295-312.
  9. 박진구. 채병윤. 여택통기탕과 여택통기탕가미방의 항알레르기 효과에 대한 실험적 연구. 동의학회지. 2001;5(1):76-97.
  10. 고정순, 김종한, 최정화, 박수연. 선여택통기탕이 ovalbumin에 의하여 유발된 allergic mouse의 면역기능에 미치는 영향. 한방안이비인후피부과학회지. 2005;18(1):94-103.
  11. Jiang C, Ting AT, Seed B. PPAR-gamma agonists inhibit production of monocyte inflammatory cytokines. Nature. 1998;391(6662):82-6. https://doi.org/10.1038/34184
  12. Jeon EJ, Lee SK, Park YS, Kim DH, Yum JH, Park CS. The effects of peroxisome proliferator-activated receptor-gamma agonist on a murine model of experimental allergic rhinitis. Otolaryngol Head Neck Surg. 2008;139(1):124-30. https://doi.org/10.1016/j.otohns.2008.03.030
  13. 대한천식및알레르기학회. 천식과 알레르기 질환. 서울:군자출판사. 2002:189.
  14. 蔡炳允. 韓方眼耳鼻咽喉科學. 서울:集文堂. 1982:321-6.
  15. 盧石善 編著. 原色眼耳卑咽喉科學. 周珉出版社. 2003:558-61.
  16. Rosenwasser L. New insights into the pathophysiology of allergic rhinitis. Allergy Asthma Proc 2007;28:10-5. https://doi.org/10.2500/aap.2007.28.2977
  17. KleinJan A. McEuen AR. Dijkstra MD. Buckley MG. Walls AF. Fokkens WJ. Basophil and eosinophil accumulation and mast cell degranulation in the nasal mucosa of pathents with hay fever after local allergen provocation J Allergy Clin Immunol 2000;106:677-86. https://doi.org/10.1067/mai.2000.109621
  18. Christodoulopoulos P, Cameron L, Durhan S, Hamid Q. Molecular pathology of allergic disease II : Upper airway disease. J Allergy Clin Immunol. 2000;105:211-23. https://doi.org/10.1016/S0091-6749(00)90068-X
  19. 이주희, 김현, 최영철, 정희재, 오태환, 정승기, 이형구. 비색증과 알레르기비염에 관한 문헌적 고찰(원인과 증상을 중심으로). 대한한방내과학회지. 1993;14(1):26-34.
  20. 曹孝忠外. 聖濟總錄. 台北:新文豊出版公司. 1978;下冊:948.
  21. 襲廷賢. 萬病回春. 서울:행림서원. 1792;卷 下:14.
  22. 서부일, 김선희, 박순달, 이극로. 白朮 黃芪 龍葵의 免疫調節作用 및 알레르기 低減化에 關한 硏究. 대한한의학방제학회. 1997;5(1):184-202.
  23. Du Q, Chen Z, Zhou LF, Zhang Q, Huang M, Yin KS. Inhibitory effects of astragaloside IV on ovalbumin-induced chronic experimental asthma. Can J Physiol Pharmacol. 2008;86(7):449-57. https://doi.org/10.1139/Y08-053
  24. Cho JH, Lee JY, Sim SS, Whang WK, Kim CJ. Inhibitory effects of diterpene acids from root of Aralia cordata on IgE-mediated asthma in guinea pigs. Pulm Pharmacol Ther. 2010;23(3):190-9. https://doi.org/10.1016/j.pupt.2009.12.004
  25. 최수형, 김영란, 임동구, 배은옥. 수종 생약의 항알레르기 작용. 약학회지. 1992;36(2):140-9.
  26. 곽상교, 박양춘. 감초가 천식모델 생쥐의 혈청내 cytokine에 미치는 효과. 동의생리병리학회지. 2004;18(2):463-7.
  27. 송상진, 배한호, 박양춘. 감초가 천식모델 생쥐의 plasma내 histamine과 폐조직내 cytokine 생성에 미치는 효과. 동의생리병리학회지. 2004;18(3):841-5.
  28. 한영주, 박양춘. 감초(Glycyrrhiza uralensis Fisch, GLU)가 천식모델 생쥐의 BALF내 면역세포 및 cytokine에 미치는 효과. 대한한방내과학회지. 2004;25(3):408-17.
  29. Liu YG, Luo JB. Effects of among compositions of Herba Ephedraedecoction on genic xpression of 5-lipoxygenase activating protein, IL-4 and leukotriene C4 in asthmatic mice. Zhongguo Zhong Yao Za Zhi. 2007;32(3):246-9.
  30. Lee J, Lim KT. Inhibitory effect of phytoglycoprotein (24kDa) on allergyrelated factors in compound 48/80-induced mast cells in vivo and in vitro. Int Immunopharmacol. 2010;10(5):591-9. https://doi.org/10.1016/j.intimp.2010.02.009
  31. Ahui ML, Champy P, Ramadan A, Pham Van L, Araujo L, Brou André K, Diem S, Damotte D, Kati-Coulibaly S, Offoumou MA, Dy M, Thieblemont N, Herbelin A. Ginger prevents Th2-mediated immune responses in a mouse model of airway inflammation. Int Immunopharmacol. 2008;8(12):1626-32. https://doi.org/10.1016/j.intimp.2008.07.009
  32. Ryu M, Kim EH, Chun M, Kang S, Shim B, Yu YB, Jeong G, Lee JS. Astragali Radix elicits anti-inflammation via activation of MKP-1, concomitant with attenuation of p38 and Erk. J Ethnopharmacol. 2008;115(2):184-93. https://doi.org/10.1016/j.jep.2007.09.027
  33. Jang MH, Shin MC, Kim YJ, Kim CJ, Kim Y, Kim EH. Atractylodes japonica suppresses lipopolysaccharide-stimulated expressions of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 macrophages. Biol Pharm Bull. 2004;27(3):324-7. https://doi.org/10.1248/bpb.27.324
  34. 이도연, 최고야, 윤태숙, 전명숙, 김승주, 야스카와켄, 유소연, 김호경. 강활과 방풍의 항염증 상승작용에 관한 연구. 대한본초학회지. 2008;23(4):39-44.
  35. Lim H, Jung HA, Choi JS, Kim YS, Kang SS, Kim HP. Anti-inflammatory activity of the constituents of the roots of Aralia continentalis. Arch Pharm Res. 2009;32(9):1237-43. https://doi.org/10.1007/s12272-009-1909-3
  36. Sakai S, Kawamata H, Kogure T, Mantani N, Terasawa K, Umatake M, Ochiai H. Inhibitory effect of ferulic acid and isoferulic acid on the production of macrophage inflammatory protein-2 in response to respiratory syncytial virus infection in RAW264.7 cells. Mediators Inflamm. 1999;8(3):173-5. https://doi.org/10.1080/09629359990513
  37. Hirabayashi T, Ochiai H, Sakai S, Nakajima K, Terasawa K. Inhibitory effect of ferulic acid and isoferulic acid on murine interleukin-8 production in response to influenza virus infections in vitro and in vivo. Planta Med. 1995;61(3):221-6. https://doi.org/10.1055/s-2006-958060
  38. 김영란, 최수형, 김경만. 승마의 항알러지작용 및 항염증작용:활성성분의 부분적 정제. 한국응용약물학회지. 1994;2(2):149-54.
  39. Kim IT, Park YM, Shin KM, Ha J, Choi J, Jung HJ, Park HJ, Lee KT. Anti-inflammatory and anti-nociceptive effects of the extract from Kalopanax pictus, Pueraria thunbergiana and Rhus verniciflua. J Ethnopharmacol. 2004;94(1):165-73. https://doi.org/10.1016/j.jep.2004.05.015
  40. Shin EM, Zhou HY, Guo LY, Kim JA, Lee SH, Merfort I, Kang SS, Kim HS, Kim S, Kim YS. Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7macrophages. Int Immunopharmacol. 2008; 8(11):1524-32. https://doi.org/10.1016/j.intimp.2008.06.008
  41. Kim JY, Park SJ, Yun KJ, Cho YW, Park HJ, Lee KT. Isoliquiritigenin isolated from the roots of Glycyrrhiza uralensis inhibits LPS-induced iNOS and COX-2 expression via the attenuation of NF-kappaB in RAW 264.7 macrophages. Eur J Pharmacol. 2008;584(1):175-84. https://doi.org/10.1016/j.ejphar.2008.01.032
  42. Lee SJ, Lim KT. Inhibitory effect of ZPDC glycoprotein on the expression of inflammation-related cytokines through p38 MAP kinase and JNK in lipopolysaccharide-stimulated RAW 264.7 cells. Inflamm Res. 2009;58(4):184-91. https://doi.org/10.1007/s00011-008-8118-2
  43. Lee SJ, Lim KT. ZPDC glycoprotein inhibits inflammation-related cytokine and protein via nuclear factor-kappa B in dextran sulfate sodium-stimulated ICR mouse. Biomed Pharmacother. 2009;63(7):528-36. https://doi.org/10.1016/j.biopha.2008.08.015
  44. 이상현, 박찬기. 芷貝散의 抗炎症 效能에 대한硏究. 대한한의학방제학회지. 2008;16(1):79-94.
  45. Lin CH, Chang CW, Wang CC, Chang MS, Yang LL. Byakangelicol, isolated from Angelica dahurica, inhibits both the activity and induction of cyclooxygenase-2 in human pulmonary epithelial cells. J Pharm Pharmacol. 2002;54(9):1271-8. https://doi.org/10.1211/002235702320402125
  46. 정환용, 주예진, 정혜미, 신우진, 서운교. 뇌신경소교세포에서 생강 헥산 분획물의 염증매개 물질 생성 억제효과. 대한한의학회지. 2009;30(2):17-29.
  47. Dugasani S, Pichika MR, Nadarajah VD, Balijepalli MK, Tandra S,Korlakunta JN. Comparative antioxidant and antiinflammatory effects of [6]-gingerol, [8]-gingerol, [10]-gingerol and [6]-shogaol. J Ethnopharmacol. 2010;127(2):515-20. https://doi.org/10.1016/j.jep.2009.10.004
  48. Ahui ML, Champy P, Ramadan A, Pham Van L, Araujo L, Brou André K, Diem S, Damotte D, Kati-Coulibaly S, Offoumou MA, Dy M, Thieblemont N, Herbelin A. Ginger prevents Th2-mediated immune responses in a mouse model of airway inflammation. Int Immunopharmacol. 2008;8(12):1626-
  49. 김희택, 남창규, 배성한. 후각장애 환자에서의 麗澤通氣湯 치험 1례. 동의생리병리학회지. 2001;15(5):830-2.
  50. Woerly G, Honda K, Loyens M, Papin JP, Auwerx J, Staels B, Capron M, Dombrowicz D. Peroxisome proliferatoractivated receptors alpha and gamma down-regulate allergic inflammation and eosinophil activation. J Exp Med. 2003 Aug 4;198(3):411-21. https://doi.org/10.1084/jem.20021384
  51. Won HY, Min HJ, Ahn JH, Yoo SE, Bae MA, Hong JH, Hwang ES. Anti-allergic function and regulatory mechanisms of KR62980 in allergen-induced airway inflammation. Biochem Pharmacol. 2010;79(6):888-96. https://doi.org/10.1016/j.bcp.2009.10.023
  52. Hammad H, de Heer HJ, Soullie T, Angeli V, Trottein F, Hoogsteden HC, Lambrecht BN. Activation of peroxisome proliferator-activated receptor-gamma in dendritic cells inhibits the development of eosinophilic airway inflammation in a mouse model of asthma. Am J Pathol. 2004; 164(1):263-71. https://doi.org/10.1016/S0002-9440(10)63116-1
  53. Koreck AI, Csoma Z, Bodai L, Ignacz F, Kenderessy AS, Kadocsa E, Szabo G, Bor Z, Erdei A, Szony B, Homey B, Dobozy A, Kemeny L. Rhinophototherapy: a new therapeutic tool for the management of allergic rhinitis. J Allergy Clin Immunol. 2005 Mar;115(3):541-7. https://doi.org/10.1016/j.jaci.2004.11.005
  54. Rousset F, Robert J, Andary M, Bonnin JP, Souillet G, Chretien I, Briere F, Pene J, de Vries JE. Shifts in interleukin-4 and interferon-gamma production by T cells of patients with elevated serum IgE levels and the modulatory effects of these lymphokines on spontaneous IgE synthesis. J Allergy Clin Immunol. 1991 Jan;87(1 Pt 1):58-69. https://doi.org/10.1016/0091-6749(91)90213-8
  55. Trifilieff A, Bench A, Hanley M, Bayley D, Campbell E, Whittaker P. PPAR-alpha and -gamma but not -delta agonists inhibit airway inflammation in a murine model of asthma: in vitro evidence for an NF-kappaB-independent effect. Br J Pharmacol. 2003;139(1):163-71. https://doi.org/10.1038/sj.bjp.0705232
  56. Hibbs JB Jr, Taintor RR, Vavrin Z. Macrophage cytotoxicity: role for L-arginine deiminase and imino nitrogen oxidation to nitrite. Science. 1987;235(4787):473-6. https://doi.org/10.1126/science.2432665
  57. Toda N, Nakanishi-Toda M. Nitric oxide: ocular blood flow, glaucoma, and diabetic retinopathy. Prog Retin Eye Res. 2007;26(3):205-38. https://doi.org/10.1016/j.preteyeres.2007.01.004
  58. Aktan F. iNOS-mediated nitric oxide production and its regulation. Life Sci. 2004;75(6):639-53. https://doi.org/10.1016/j.lfs.2003.10.042
  59. Radi R., Beckman J. S., Bush K. M., Freeman B. A.. Peroxynitrite-induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide. Arch Biochem Biophys. 1991;1;288(2):481-7. https://doi.org/10.1016/0003-9861(91)90224-7
  60. Villa L. M., Salas E., Darley-Usmar V. M., Radomski M. W., Moncada S.. Peroxynitrite induces both vasodilatation and impaired vascular relaxation in the isolated perfused rat heart. Proc Natl Acad Sci USA. 1994;20;91(26):12383-7. https://doi.org/10.1073/pnas.91.26.12383
  61. Robbins RA, Grisham MB. Nitric oxide. Int J Biochem Cell Biol 1997;29:857-904. https://doi.org/10.1016/S1357-2725(96)00167-7
  62. Honda K, Marquillies P, Capron M, Dombrowicz D. Peroxisome proliferatoractivated receptor gamma is expressed in airways and inhibits features of airway remodeling in a mouse asthma model. J Allergy Clin Immunol. 2004;113(5):882-8. https://doi.org/10.1016/j.jaci.2004.02.036
  63. Sugiyama H, Nonaka T, Kishimoto T, Komoriya K, Tsuji K, Nakahata T. Peroxisome proliferator-activated receptors are expressed in human cultured mast cells: a possible role of these receptors in negative regulation of mast cell activation. Eur J Immunol. 2000;30(12):3363-70. https://doi.org/10.1002/1521-4141(2000012)30:12<3363::AID-IMMU3363>3.0.CO;2-B
  64. Pfaar O, Raap U, Holz M, Hormann K, Klimek L. : Pathophysiology of itching and sneezing in allergic rhinitis. 2009;139(3-4):35-40.
  65. Misery L. Skin, immunity, and nervous system. Br J Dermatol. 1997;137:843-59. https://doi.org/10.1111/j.1365-2133.1997.tb01542.x
  66. Sherry et al. Resolution of the two components of macrophage inflammatory protein 1, and cloning and characterization of one of those components, macrophage inflammatory protein 1-beta. J. Exp. Med. 1988;168:2251-9. https://doi.org/10.1084/jem.168.6.2251
  67. Irving et al. Two inflammatory mediator cytokine genes are closely linked and variably amplified on chromosome 17q. Nucleic Acids Res. 1990;18:3261-70. https://doi.org/10.1093/nar/18.11.3261
  68. Yoshida S., Ono M., Shono T., Izumi H., Ishibashi T., Suzuki H., Kuwano M. : Involvement of IL-8, vascular endothelial growth factor, and basic fibroblast growth factor in $TNF-\alpha$ dependent angiogenesis. Mol. Cell Biol. 1997;17:4015-23. https://doi.org/10.1128/MCB.17.7.4015