The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Effects of atopic dermatitis induced materials on the expression of cytokine genes in human monocytes and mast cells

인간의 단핵구와 비만세포에서 다양한 아토피 유발물질이 사이토카인 유전자의 발현에 미치는 영향

  • 박경숙 (경원대학교 한의과대학 안이비인후피부과학 교실) ;
  • 김경준 (경원대학교 한의과대학 안이비인후피부과학 교실)
  • Received : 2010.07.09
  • Accepted : 2010.08.03
  • Published : 2010.08.25
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Abstract

Objectives : On an experimental basis, the effects of atopic dermatitis induced materials on the expression of cytokine genes in human monocytes (THP-1, U937) and mast cells were studied. This study was carried out to be considered a fundamental knowledge in the research on the good of oriental medicine. Methods : After culturing THP-1, U937, and HMC-1, with the three different concentrations of LPS ($1\;{\mu}g/ml$), DPE ($10\;{\mu}g/ml$), and DNCB ($1\;{\mu}g/ml$), atopic dermatitis induced materials were treated in the culture medium. To investigate cytokine genes expression patterns, with lysis buffer and separation reagent, total RNA was extracted from THP-1, U937, and HMC-1 at intervals of 0, 12, 24, and 48 hours. Both cytokine mRNA expression patterns by atopic dermatitis induced materials and change of cytokine genes expression patterns in relation to atopy by selenium were analyzed with RT-PCR. Also IL-4 and INF-$\gamma$, which were secreted in the HMC-1, were analyzed using ELISA method. Results : 1. After treating THP-1 and U937 with LPS, DPE, and DNCB, there was no significant change in cytokine genes themselves, but various cytokines (IL-4, IL-6, IL-8, IL-13, IFN-$\gamma$, IFN-a, MCP-1, B2-MG) were expressed. 2. In the case of HMC-1, the expressions of IL-6 and IL-8 were significantly increased in the analysis of mRNA expression by dust mite allergens in DPE. 3. As a result of ELISA method, it is certain that IL-4 and IFN-$\gamma$ protein were secreted in the HMC-1 by DPE. 4. Selenium, an essential trace element, decreased the IL-10 and IL-13 expression in the HMC-1 by DPE. Conclusion : The results suggest that it is necessary to choose proper atopic dermatitis induced materials and suitable cultured cells in establishment of in vitro model of atopic dermatitis.

Keywords

References

  1. Mc daniel ML, Kwon G, Hill JR, Marshall CA and Corbett JA. Cytokines and nitric oxides in islet inflammation and diabetes. Proc. Soc. Exp. Biol. Med 1996;211:24-32. https://doi.org/10.3181/00379727-211-43950D
  2. Higuchi M, Higashi N, Taki H, Osawa T. Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine dependent mechanism acts as synergistically as the major cytolytic mechanism of activated macrophages. J Immunol. 1990;144:1425-31.
  3. Corbett JA, and Mac Daniel ML. Intraislet release of interleukin-1 inhibits beta cell function by inducing beta cell expression of inducible nitric oxide syntheses. J Exp Med. 1995;181:559-68. https://doi.org/10.1084/jem.181.2.559
  4. Cetkovic-Cvrlje M and Eizirik DL. TNF and $IFN-\gamma$ potentiate the deleterious effects of $IL-1\beta$ on mouse pancreatic islets mainly via generation of nitric oxide. Cytokine. 1994;6:399-406. https://doi.org/10.1016/1043-4666(94)90064-7
  5. 김정원. 알레르기 및 면역학적 관점에서의 아토피피부염. 대한피부과학회지. 2003;41(6):687-9.
  6. 안성구. COMMON SKIN DISEASE. 서울: 퍼시픽출판사. 2003:68.
  7. 박용민. 아토피피부염 병태생리에 대한 최신지견. 소아알레르기 및 호흡기. 2006;16(3):189-96.
  8. Borich L. Allergic rhinitis: Systemic inflammation and implications for management. J Allergy Clin Immunol. 2002:60-2.
  9. Yang SH, Hong CY, Yu CL. Decreased serum IgE level, decreased $INF-\gamma$ and IL-5 but increased IL-10 production, and suppresed cyclooxygenase 2 mRNA expression in patients with perennial allergic rhinitis after treatment with a new mixed formula of Chinese herbs. Int Immunopharmacol. 2001;1(6):1173-82. https://doi.org/10.1016/S1567-5769(01)00051-0
  10. Chrousos GP. Stress, chronic inflammation, and emotional and physical well-being: Concurrent effects and chronic sequelae. J Allergy Clin Immunol 2000;106:S275-91. https://doi.org/10.1067/mai.2000.110163
  11. Friedmann PS, Tan BB, Musaba E, Pathogenesis and management of atopic dermatitis. Clin. Exp. Allergy. 1995;25:799-806. https://doi.org/10.1111/j.1365-2222.1995.tb00021.x
  12. Koreck AI, Csoma Z, Bodai L, Ignacz F, Kenderessy AS, Kadocsa E, Szabo G, BorZ, Erdei A, Szony B, Homey B, Dobozy A, Kemedy L. Rhinophototherapy: A new therapeutic tool for themanagement of allergic rhinitis. J Allergy Clin Immunol 2005;115:541-7. https://doi.org/10.1016/j.jaci.2004.11.005
  13. Brown KM, Arthur JR. Selenium, Selenoproteins, and human health: a review. Public Health Nutrition 2001;4:593-9.
  14. Navaro-alarcon N, Lopez-Martinez MC. Essentiality of selenium in the human body: relationship with different disease. Sci Total Environ 2000;249:347-71. https://doi.org/10.1016/S0048-9697(99)00526-4
  15. 이명희. 셀레늄 영양과 건강. 대한암예방학회지 2003;8:36-44.
  16. Perez A, Neve J, Desmedt J, Duchateau J, Dramaix M, Famaey JP. Lymphocytes response is enhanced by supplementation of elderly subjects with selenium enriched yeast. Am J Clin Nutr 1991;53;1323-8.
  17. Kiremidjian-Schmacher L, Roy M, Wishe HL, Cohen MW, Stotzky G. Supplementation with selenium and human immune cell functions. Biol Trace Elem Res 1994;41:115-27. https://doi.org/10.1007/BF02917222
  18. Kiremidjian-Schmacher L, Roy M, Wishe HL, Cohen MW, Stotzky G. Supplementation with selenium arguments the functions of natural killer and lymphokine activated killer cells. Biol Trace Elem Res 1996;52:227-39. https://doi.org/10.1007/BF02789164
  19. Heinle K, Adam A, Gradl M, Wiseman M, Adam O. Selenium concentration in erythrocytes of patients with rheumatoid arthritis. Clinical and laboratory chemistry infection markers during administration of selenium. Med Klin 1997;Suppl 3:29-3.
  20. Lee JS, Kim IS, Ryu JS, Yun CY. House dust mite, Dermatophagoides pteronissinus increases expression of MCP-1, IL-6, and IL-8 in human monocytic THP-1 cells. Cytokine. 2008;42:365-71. https://doi.org/10.1016/j.cyto.2008.03.010
  21. Kim DB, Kim JH, Kwon SH, Kim YJ, Lee SH, Lee YH, Seo JN, Park CS, Park KL, Kwon HJ. Regulation of macrophage inflammatory protein-2 gene expression in response to 2,4-dinitrofluorobenzene in RAW264.7 cells. 2008;41(4):316-21.
  22. 대한피부과학회 교과서 편찬위원회. 개정4판 피부과학. 서울:여문각. 2001:161-6.
  23. 나영호. 아토피피부염 발병기전에서 케모카인의 역할. 소아알레르기 및 호흡기학회지. 2005;15(3):238-41.
  24. 김정희. 아토피피부염의 최신 지견. 소아알레르기 및 호흡기학회지. 2004;14(1):12-23.
  25. Qgawa H, Yoshiike T. A speculative view of atopic dermatitis: barrier dysfunction in pathogenesis. J Dermatol Sci. 1993;5:197-204. https://doi.org/10.1016/0923-1811(93)90767-J
  26. Paul WE. Interleukin-4: a prototypic immunoregulatory lymphokine. Blood 1991;77:1859–70.
  27. Heijink IH, Van Oosterhout AJ. Targeting T cells for asthma. Curr Opin Pharmacol 2005;5:227–31. https://doi.org/10.1016/j.coph.2005.04.002
  28. Homey B, Steinhoff M, Ruzicka T, Leung DY. Cytokines and chemokines orchestrate atopic skin inflammation. J Allergy Clin Immunol 2006;118:178–89. https://doi.org/10.1016/j.jaci.2006.03.047
  29. Arlian LG, Platts-Mills TA. The biology of dust mites and the remediation of mite allergens in allergic disease. J Allergy Clin Immunol 2001;107:S406–S413. https://doi.org/10.1067/mai.2001.113670
  30. Bellanti JA. Cytokines and allergic diseases: clinical aspects. Allergy Asthma Proc 1998;19:337-41. https://doi.org/10.2500/108854198778612735
  31. Williams CM, Galli SJ. The diverse potential effector and immunoregulatory roles of mast cells in allergic disease. J Allergy Clin Immunol 2000;105:847–59. https://doi.org/10.1067/mai.2000.106485
  32. Theoharides TC, Cochrane DE. Critical role of mast cells in inflammatory diseases and the effect of acute stress. J Neuroimmunol 2004;146:1-12. https://doi.org/10.1016/j.jneuroim.2003.10.041
  33. Bisset LR, Schmid-Grendelmeier P. Chemokines and their receptors in the pathogenesis of allergic asthma: progress and perspective. Curr Opin Pulmon Med 2005;11:35–42. https://doi.org/10.1097/01.mcp.0000144502.50149.e0
  34. Na HJ, Shin HY, Kim NH, Kwon MW, Park EJ, Hong SH, Kim NI, Kim HM. Regulatory effects of cytokine production in atopic allergic reaction by Gammidanguieumja. Inflammation 2004;28:291-8. https://doi.org/10.1007/s10753-004-6053-0
  35. Kim IS, Kim JH, Kim JS, Yun CY, Kim DH, Lee JS. The inhibitory effect of Houttuynia cordata extract on stem cell factor-induced HMC-1 cell migration. J Ethnopharmacol 2007;112(1):90-5. https://doi.org/10.1016/j.jep.2007.02.010
  36. Lee JS, Kim IS, Kim JH, Kim JS, Kim DH, Yun CY. Suppressive effects of Houttuynia cordata Thunb (Saururaceae) extract on Th2 immune response. J Ethnopharmacol 2008;117(1):34-40. https://doi.org/10.1016/j.jep.2008.01.013
  37. Shin TY, Kim SH, Kim DK, Leem KH,Park JS. Phlomis umbrosa root inhibits mast cell-dependent allergic reactions and inflammatory cytokine secretion. Phytotherapy Res 2008;22:153-8. https://doi.org/10.1002/ptr.2164
  38. Hataguchi Y, Tai H, Nakajima H, Kimata H. Drinking deep-sea water restores mineral imbalance in atopic eczema/dermatitis syndrome. Eur J Clin Nutr 2005;59:1093-6. https://doi.org/10.1038/sj.ejcn.1602218
  39. Shirmali RK, Iron RD, Carlson BA, Sano Y, Gladyshev VN, Park JM, Hatfield DL. Selenoproteins mediate T cell immunity through an antioxidant mechanism. J Biol Chem 2008;283:20181-5. https://doi.org/10.1074/jbc.M802559200
  40. Obara W, Kawa Y, Ra C, Nishioka K, Soma Y, Mizoguchi M. T cells and mast cells as a major source of interleukin-13 in atopic dermatitis. Dermatology 2002;205:11-7. https://doi.org/10.1159/000063145