Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Enhancement of Skin-Whitening and UV-Protective Effects of Centella asiatica L. Urban by Utrasonification Process

초음파 병행을 통한 병풀의 미백 및 자외선 차단 활성 증진 효과

  • Ha, Ji-Hye (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Kwon, Min-Chul (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Kim, Seung-Seop (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Jeong, Myoung-Hoon (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Hwang, Baik (Department of Biology, Chonnam National University) ;
  • Lee, Hyeon-Yong (School of Biotechnology and Bioengineering, Kangwon National University)
  • 하지혜 (강원대학교 바이오산업공학부) ;
  • 권민철 (강원대학교 바이오산업공학부) ;
  • 김승섭 (강원대학교 바이오산업공학부) ;
  • 정명훈 (강원대학교 바이오산업공학부) ;
  • 황백 (전남대학교 생물학과) ;
  • 이현용 (강원대학교 바이오산업공학부)
  • Received : 2010.01.11
  • Accepted : 2010.04.05
  • Published : 2010.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Enhancement effect of ultrasonification process on UV-protection and skin-whitening activities using Centella asiatica L. Urban extract was investigated. Cytotoxicity of the extracts measured on human skin fibroblast cells, CCD-986sk, and then, ultrasonification associated extracts showed 5~9% lower cytotoxicity then normal crude extracts on 1.0 mg/$m{\ell}$ of highest sample concentration. The associated extrats showed highest inhibition activity of hyaluronidase on 1.0 mg/$m{\ell}$ of concentration as 54.2%. Also, the associated extract reduced expression of MMP-1 on UV-irradiated CCD-986sk cells down to 100.2% from 136.1%, and revealed high inhibitory potency on tyrosinase as 74.6% by adding 1.0 mg/$m{\ell}$ of concentration. Ultrasonification associated extract showed strong inhibition effect of melanin production on Clone M-3 cells as 84.2% by adding 1.0 mg/$m{\ell}$ of concentration. From the preliminary observations, we considered that the extracts from C. asiatica could be potent natural materials for skin-whitening and anti-aging agent, and could enhance the activities by ultrasonification process.

Keywords

References

  1. Bonte F, Dumas M, Chaudagne C and Meybeck A. (1993). Influence of asiatic acid, madecassiac acid, and asiaticoside on human collagen I syntheses. Planta Medica. 60:133-135.
  2. Brinkhaus B, Linder M, Schuppan D and Hahn EG. (2000). Chemical, pharmacological and clinical profile of the East Asian medical plant Centella asiatica. Phytomedicine. 7:427-448. https://doi.org/10.1016/S0944-7113(00)80065-3
  3. Chung K, Kim WI, Hong IK and Park KA. (2000). Ultrasonic energy effects on squalene extraction from amaranth seed. Applied Chemistry. 4:149-152.
  4. Doll R. and Peto R. (1983). The causes of cancer: Quantitative estimates of avoidable risks of cancer in the United States today. Food and Chemical Toxicology. 21:512-513.
  5. Dunsmore SE, Rubin JS, Kovacs SO, Chedid M, Parks WC and Welgus HG. (1996). Mechanisms of hepatocyte growth factor stimulation of keratinocyte metalloproteinase production. Journal of Biological Chemistry. 271:24576-24582. https://doi.org/10.1074/jbc.271.40.24576
  6. Gilchrest BA. (1989). Skin aging and photoaging. Journal of the American Academy of Dermatology. 21:610-613. https://doi.org/10.1016/S0190-9622(89)70227-9
  7. Hearing VJ. (1987). Mammalian monophenol monooxygenase (Tyrosinase): Purification, properties, and reaction catalyzed. Methods in Enzymology. 142:154-165. https://doi.org/10.1016/S0076-6879(87)42024-7
  8. Kartnig T and Hoffmann-Bohm K. (1992). Centella. In : Hansel R, Keller K, Rimpler H, Schneider (eds) Hager's Handbuch der Pharmazeutischen Praxis. Band 4. Springer Verlag, Berlin. (Hausen).
  9. Kim OT, Kim MY, Kim SJ, Kim YJ, Kim KS, Ahn JC, Kim SW and Hwang B. (2002). Seasonal variations of triterpene glycosides contents in the leaf of Centella asiatica (L.) Urban. Korean Journal of Medicinal Crop Science. 10:375-378.
  10. Kim WI, Shung KW, Lee SB, Hong IK and Park KA. (2001). Ultrasound energy effects on solvent extraction of amaranth seed oil. Journal of the Korean Industrial and Engineering Chemistry. 12:307-311.
  11. Kwon MC, Han JG, Ahn JH, Lee DH, Syed Abdul Q and Lee HY. (2008). Enhancement of immuno-potentiation of Cichorium endivia L. by ultrasonification extraction process. Korean Journal of Medicinal Crop Science. 16:1-7.
  12. Lee SJ, Park DW, Jang HG, Kim CY, Park YS, Kim TC and Heo BG. (2006). Total phenol content, electron donating ability, and tyrosinase inhibition activity of pear cut branch extract. Korean Journal of Horticulture Science and Technology. 24:338-341.
  13. Lim E, Browning J, MacGregor D, Davis ID and Cebon JS. (2006). Desmoplastic melanoma : comparison of expression of differentiation antigens and cancer testis antigens. Melanoma Research. 16:347-355. https://doi.org/10.1097/01.cmr.0000222588.22493.3f
  14. Park YH and Kim MR. (1995). A study on the tyrosinase inhibitor from mushroom phellinus ribis. Soonchunhyang Journal of Natural Science. 1:183-188.
  15. Park JH, Lee HS, Mun HC, Kim DH, Seong NS, Jung HG, Bang JK and Lee HY. (2004). Improvement of Anticancer Activation of Ultrasonificated Extracts from Acanthopanax senticosus Harms, Ephedra sinica Stapf, Rubus coreanus Miq. and Artemisia capollaris Thunb. Korean Journal of Medicinal Crop Science. 12:273-278.