Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Anticancer Activity of Acer mono Wood Extracted by Ultra High Pressure Extraction Process

초고압 추출 공정을 통한 고로쇠 목부 추출물의 항암활성 증진

  • Jeong, Myoung-Hoon (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Choi, Woon-Yong (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Seo, Yong-Chang (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Kang, Ha-Young (Korea Forest Research Institute) ;
  • Choi, Geun-Pyo (Department of Food Processing & Bakery, Gangneung Provincial University) ;
  • Lee, Hyeon-Yong (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University)
  • 정명훈 (강원대학교 바이오산업공학부 생물소재공학과) ;
  • 최운용 (강원대학교 바이오산업공학부 생물소재공학과) ;
  • 서용창 (강원대학교 바이오산업공학부 생물소재공학과) ;
  • 강하영 (국립산림과학원) ;
  • 최근표 (강원도립대학 식품가공제과제빵과) ;
  • 이현용 (강원대학교 바이오산업공학부 생물소재공학과)
  • Received : 2010.04.19
  • Accepted : 2010.06.08
  • Published : 2010.06.30
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Abstract

We investigated a method to improve anticancer activities of Acer mono wood extracts by ultra high pressure extraction process. The A. mono was extracted by water at $40^{\circ}C$ and 300 MPa for 15 min (High Pressure Extraction, HPE). The extraction yield by ultra high pressure extraction process was 5.42%. The cytotoxicity on human normal lung cell (HEL299) of the extracts from HPE showed 21.54% lower than that from conventional water extraction at $100^{\circ}C$ in adding the maximum concentration of 1.0 mg/$m{\ell}$. Ultra high pressure extracts process for 15 minutes extracts (HPE15) showed more potent scavenging effect than the control, BHA. On SOD-like test, the HPE15 showed highest activity as 32.4% at 1.0 mg/$m{\ell}$ concentration. Human stomach adenocarcinoma, liver adenocarcinoma, breast adenocarcinoma and lung adenocarcinoma cell growth were inhibited up to about 67~79%, in adding 1.0 mg/$m{\ell}$ of extracts from HPE. HPE was 20~25% higher than conventional water extraction. It was interesting that, among several cancer cell lines (stomach adenocarcinoma, liver adenocarcinoma), the growth of digestive related cancer cells were most effectively inhibited as about 75~79%. On in vivo experiment using ICR mice, the variation of body weight of mice group treated A. mono wood extracts from HPE of 100 mg/kg/day concentration was very lower than control and other group. The survival times of group treated this extracts was 61.96% longer than that of the control group and this extracts showed the lower tumor weight, which were 10.49 g than positive control as 16.17 g. Based on these results, we could tell that the HPE wood extracts of A. mono had higher anticancer activity than conventional water extraction. The results of HPE showed obvious advantages in higher efficiency, shorter extraction time, at lower energy costs.

Keywords

References

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