흑마늘 농축액을 첨가한 요구르트의 제조와 품질특성

Preparation and Quality Characteristics of Yogurt with Black Garlic Extracts

  • 신정혜 ((재)남해마늘연구소) ;
  • 김경민 ((재)남해마늘연구소) ;
  • 강민정 ((재)남해마늘연구소) ;
  • 양승미 (경남도립남해대학 호텔조리제빵과) ;
  • 성낙주 ((재)남해마늘연구소.경상대학교 식품영양학과)
  • Shin, Jung-Hye (Namhae Garlic Research Institute) ;
  • Kim, Gyoung-Min (Namhae Garlic Research Institute) ;
  • Kang, Min-Jung (Namhae Garlic Research Institute) ;
  • Yang, Seung-Mi (Department of Hotel Culinary Arts & Bakery, Gyeongnam Provinical Namhae College) ;
  • Sung, Nak-Ju (Namhae Garlic Research Institute and Department of Food Science and Nutrition, Gyeongsang National University)
  • 투고 : 2010.04.29
  • 심사 : 2010.05.31
  • 발행 : 2010.06.30

초록

흑마늘 농축액을 첨가한 요구르트의 품질특성 평가를 위하여 탈지분유에 0.5~1.5% (w/v) 흑마늘 농축액을 혼합하고 Streptococcus thermophilus와 Lactobacillus delbrueckii subsp. bulgaricus의 혼합균주를 접종하여 $42^{\circ}C$에서 24시간 배양하였다. 흑마늘 농축액 첨가량에 따른 유산균의 생육정도, 요구르트의 이화학적 및 관능적 특성을 검토하였다. 흑마늘 농축액이 첨가된 요구르트의 pH는 발효 6시간 이후부터 pH 6.0 이하로 대조군에 비해 더 빨리 산성화 되었으며, 발효 24시간에 적정산도는 흑마늘 농축액의 첨가량이 높을수록 증가율이 더 높아 흑마늘 농축액 1.5% 첨가군의 경우 발효 전에 비해 약 5.7배 증가하여 0.74%였다. 흑마늘 농축액을 첨가한 요구르트의 생균수는 발효 3시간까지는 흑마늘 첨가량이 증가할수록 생육정도가 감소하였으나 발효시간이 경과함에 따라 대조군과 유의적인 차이가 없었다. 흑마늘 농축액의 첨가량이 증가할수록 요구르트의 점도는 감소하는 경향이었으나 통계적인 유의차는 없었고, 색도는 흑마늘 농축액의 첨가량이 증가할수록 명도(L값)는 감소하였고 적색도(a값)와 황색도(b값)는 증가하였다. 흑마늘 농축액의 첨가량이 증가할수록 전체적인 기호도가 감소하였는데 이상의 모든 분석 결과를 종합하여 볼 때 요구르트 제조시 흑마늘 농축액의 적정 첨가 농도는 1% 미만이 적합한 것으로 판단된다.

Yogurt base was prepared from skim milk added with 0.5, 1.0 and 1.5% (w/v) black garlic extracts(BGE, 60 brix), fellowed by fermentation with Lactic acid bacteria (the mixed strain of Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus) at $42^{\circ}C$ for 24 hr. The yogurt products were evaluated for acid production (pH, titrable acidity), number of viable cells, viscosity, color value, and sensory properties. The pH of yogurt with BGE was below 6.0 at 6 hr fermentation, after which it was rapidly acidifies. Aftert 24 hr, the titratable acidity of yogurt with 1.5% BGE was 0.74%, which was 5.7 times higher than that before fermentation. There was no significant difference in viable cell count between the samples after 3 hr fermentation. The viscosity of yogurt was decreased by the addition of BGE. As the percentage of BGE increased, the L value (lightness) decreased while the a value (redness) and b value (yellowness) increased. The overall sensory score of yogurt with BGE was lower than that of yogurt with only skim milk. Therefore, moderate addition of BGE was below 1% for the preparation of yogurt.

키워드

참고문헌

  1. Amagase H, Milner JA. 1993. Impact of various sources of garlic and their constituents on 7,12-DMBA binding to mammary cell DNA. Carcinogenesis 14(8):1627-1631 https://doi.org/10.1093/carcin/14.8.1627
  2. Amagase H, Petesch BL, Matsuura H, Kasuga S, Itakura Y. 2001. Intake of garlic and its bioactive components. Journal of Nutrition 131(3):955S-962S
  3. Awazu S, Horie T. 1997. Antioxidants in garlic II. Protection of heart mitochondria by garlic extract and diallyl polysulfide from the doxorubicin-induced lipid peroxidation. In: Nutraceuticals designer foods III garlic, soy and licorice. Lanchance, P. A. ed. Food & Nutrition Press, Trumbull, CT. pp 131-138
  4. Bae JH, Hong KR, Oh DH, Park JR, Choi SH. 2000. Fermentation characteristic of set-type yoghurt from milk added with mugwort extract. Korean J Food Sci 20(1):21-29
  5. Block E. 1985. The chemistry of garlic and onion. Sci Am 252(3):114-119 https://doi.org/10.1038/scientificamerican0385-114
  6. Chameber JV. 1979. Culture and processing techniques important to the manufacture of good quality yogurt. Cult Dairy Prod J 14(1):28-34
  7. Cheng HH, Jung TC. 1981. Effect of allithiamine on sacroma- 180 tumor growth in mice. Journal of the Formosan Medical Association 80(4):385-392
  8. Cho JR, Kim JH, In MJ. 2007. Effect of garlic powder on preparation and quality characteristics of yogurt. J Korean Soc Appl Biol Chem 50(1):48-52
  9. Cho YS, Kim SI, Han YS. 2008. Effect of slander glasswort extract yogurt on quality during storage. Korean J Food Cookery Sci 24(2):212-221
  10. Choi DJ, Lee SJ, Kang MJ, Cho HS, Sung NJ, Shin JH. 2008. Physicochemical characteristics of black garlic(Alluium sativum L.). J Korean Soc Food Sci Nutr 37(4):465-471 https://doi.org/10.3746/jkfn.2008.37.4.465
  11. Hood SK, Zottola EA. 2006. Effect of low pH on the ability of Lactobacillus acidophilus to survive and adhere to human intestinal cells. J Food Sci 53(5):1514-1516
  12. Horie T, Awazu S, Itakura Y, Fuwa T. 1992. Identified diallyl polysulfides from an aged garlic extract which protects the membranes from lipid peroxidation. Planta Med 58(5):468- 469 https://doi.org/10.1055/s-2006-961517
  13. Ichikawa M, Yoshida J, Ide N, Sasaoka T, Yamaguchi H, Ono K. 2006. Tetragydro- beta -carboline derivatives in aged garlic extract show antioxidant properties. J Nutr 136(3): 726S-731S
  14. Ide N, Lau BH. 1999. Aged garlic extract attenuates intracellular oxidative stress. Phytomedicine 6(2):125-131 https://doi.org/10.1016/S0944-7113(99)80047-6
  15. Imai J, Ide N, Nagae S, Moriguchi T, Matsuura H, Itakura Y. 1994. Antioxidant and radical scavenging effects of aged garlic extract and its constituents. Planta Med 60(5):417- 420 https://doi.org/10.1055/s-2006-959522
  16. Jain RC, Vyas CR. 1975. Garlic in alloxan-induced diabetic. Am J Clinical Nutrition 28(7):684-685
  17. Kleijnen J, Knipschild P, Riet GT. 1989. Garlic, onions and cardiovascular risk factors. A review of the evidence from human experiments with emphasis on commercially available preparations. Br J Clin Pharmacol 28(5):535-544 https://doi.org/10.1111/j.1365-2125.1989.tb03539.x
  18. Ko SH, Kim SI, Han YS. 2008. The quality characteristics of yogurt add supplemented with low grade dried-persimmon extracts. Korean J Food Cookery Sci 24(6):735-741
  19. Lee JH, Hwang HJ. 2006. Quality Characteristics of Curd Yogurt with Rubus coreanum Miquel juice. Korean J Culinary Research 12(2):195-205
  20. Lee YJ, Kim SI, Han YS. 2008. Antioxidant activity and quality characteristics of yogurt added yuza(Citrus junos Sieb ex Tanaka) extract. Korean J Food & Nutr 21(2):135-142
  21. Medina-Campos ON, Barrera D, Segoviano-Murillo S, Rocha D, Maldonado PD, Mendoza-Patiñ N, Pedraza-Chaverri J. 2007. S-allylcysteine scavenges singlet oxygen and hypochlorous acid and protects LLC-PK1 cells of potassium dichromateinduced toxicity. Food Chem Toxicol 45(10):2030-2039 https://doi.org/10.1016/j.fct.2007.05.002
  22. Nagae S, Ushijima M, Hatono S, Imai J, Kasuga S, Matsuura H, Itakura Y, Higashi Y. 1994. Pharmacokinetics of the garlic compound S-allylcysteine. Planta Med 60(3):214-217 https://doi.org/10.1055/s-2006-959461
  23. Rasic JL, Kurmann JA. 1978. Yoghurt. Scientific grounds, technology, manufacture and preparations. Technological Dairy Publishing House. Copenhagen, Denmark pp 6
  24. Robinson RK. 1981. Dairy microbiology II. The microbiology of milk products. Applied Science Publishers Ltd. London, UK pp 113-156
  25. Shashikanth KN, Basappa SC, Murthy VS. 1984. A comparative study of raw garlic extract and tetracycline on caecal microflora and serum proteins of albino rats. Folia Microbiol 29(4):348-352 https://doi.org/10.1007/BF02875970
  26. Shin JH, Choi DJ, Lee SJ, Cha JY, Sung NJ. 2008. Antioxidant activity of balck garlic(Allium savivum L.). J Korean Soc Food Sci Nutr 37(8):965-971 https://doi.org/10.3746/jkfn.2008.37.8.965
  27. So MH. 1985. Identification and tolerance test to digestive fluids of Lactobacilli isolated from Korean liquid yogurts. Korean J Food Sci Technol 17(3):192-196
  28. Sung YM, Cho JR, Oh NS, Kim DC, In MJ. 2005. Preparation and quality characteristics of curd yogurt added with chlorella. J Korean Soc Appl Biol Chem 48(1):60-64
  29. Taminme AY, Robinson RK. 1999. Yoghurt; science and technology. Woodhead publishing Ltd and CRC press LLC. Cambridge, England pp 273-286
  30. Walton I, Herbold M, Lindegren CC. 1936. Bactericidal effect of vapors from crushed garlic. Food Res 1(1):163-169
  31. 한국식품공업협회. 2009. 식품공전. (주)문영사. 서울, 대한민국 pp 110-111