Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Effects of Forsythiae Fructus on CRF, c-Fos and TH in the Forced Swimming Test

연교(連翹)가 강제수영부하시험에서 CRF, c-Fos와 TH에 미치는 영향

  • Kim, Hyo-Joon (Formulae Pharmacology Dept. School of Oriental Medicine, Kyungwon University) ;
  • Lee, Tae-Hee (Formulae Pharmacology Dept. School of Oriental Medicine, Kyungwon University)
  • 김효준 (경원대학교 한의과대학 방제학교실) ;
  • 이태희 (경원대학교 한의과대학 방제학교실)
  • Received : 2010.04.02
  • Accepted : 2010.06.10
  • Published : 2010.06.30
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Abstract

Objectives : In order to investigate the antidepressant effects of Forsythiae Fructus(FF), we performed the Forced Swimming Test(FST). Also the expressions of corticotropin-releasing factor (CRF), c-Fos and tyrosine hydroxylase(TH) were measured by immunohistochemical method at paraventricular nucleus(PVN), ventral tegmental area(VTA) and locus coeruleus(LC). Methods : Spraque-Dawley rats were administered FF extract(100 mg/kg, 400 mg/kg) intragastrically three times prior to the FST. Results : The duration of immobility in the FST was significantly decreased in the FF 100 mg/kg, 400 mg/kg groups(p<0.05). The expression of CRF was significantly reduced in the FF 100 mg/kg and 400 mg/kg groups(p<0.001). c-Fos expression was significantly decreased at PVN in the FF 100 mg/kg group(p=0.069). TH expression at VTA was significantly increased in the FF 100 mg/kg and 400 mg/kg groups(p<0.05). TH expression at LC was not significantly changed(p=0.346). Conclusion : According to the results, it can be suggested that Forsythiae Fructus has antidepressant effect via the decreased immobility through the reduction of CRF and c-Fos expression at PVN.

Keywords

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