Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
권호 표지

Effect of Water Extract of Peonia Suffruticosa and Prunus Percica on Anti-inflammation

목단피((牧丹皮).도인(桃仁) 배합(配合)이 항염증(抗炎症) 작용(作用)에 미치는 영향

  • Kim, Young-Il (Noockdang Oriental Clinic) ;
  • Lee, Sung-Jun (Dept. of Oriental Medical Prescription, Wonkwang University) ;
  • Huh, Jin (Dept. of Oriental Medical Prescription, Wonkwang University) ;
  • Lee, Tae-Hyung (Dept. of Oriental Medical Prescription, Wonkwang University) ;
  • Shin, Dong-Gean (Dept. of Oriental Medical Prescription, Wonkwang University) ;
  • Lee, Jae-Cheol (Dept. of Oriental Medical Prescription, Wonkwang University) ;
  • Shin, Yong-Seo (H&BT Co.,Ltd R/D Center) ;
  • Yun, Young-Gab (Dept. of Oriental Medical Prescription, Wonkwang University)
  • 김영일 (노옥당한의원) ;
  • 이성준 (원광대학교 한의과대학 방제학교실) ;
  • 허진 (원광대학교 한의과대학 방제학교실) ;
  • 이태형 (원광대학교 한의과대학 방제학교실) ;
  • 신동근 (원광대학교 한의과대학 방제학교실) ;
  • 이재철 (원광대학교 한의과대학 방제학교실) ;
  • 신용서 ((주)에이치앤비티 R/D센터) ;
  • 윤용갑 (원광대학교 한의과대학 방제학교실)
  • Received : 2010.05.19
  • Accepted : 2010.06.12
  • Published : 2010.06.30
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Abstract

Paeonia Suffruticosa and Prunus Persica have been used as oriental medicine for removal of fever, alleviation of pain, an anti-phlogistic effect and removal of extravasated blood. However, it has been never shown the effects of these herbal medicines on anti-inflammatory processes. This experiment was performed to show how these herbs could act as anti-inflammatory medicines at cellular level. Anti-inflammation effects of water extracts from Paeonia Suffruticosa and Prunus Persica as well as their mixture have been investigated, and the results were follows; 1) each extract slightly suppressed the expression and production of inflammatory mediators and enzymes such as NO, iNOS, IL-$1{\beta}$, and TNF-$\alpha$ in lipopolysaccharid(LPS)-stimulated RAW264.7 cells and mouse primary peritoneal macrophages in a dose-dependent manner. These suppressive effects, however, were synergistically increased by their mixture. 2) Each extract of Paeonia Suffruticosa and Prunus Persica insignificantly suppressed the activation and activity of NF-${\kappa}B$ in LPS-stimulated RAW264.7 cells, which controls the expression of inflammatory mediators such as NO, iNOS, IL-$1{\beta}$, and TNF-$\alpha$. However, extract mixture of Paeonia Suffruticosa and Prunus Persica suppressed effectively the activation and activity of NF-${\kappa}B$. 3) Each of Paeonia Suffruticosa and Prunus Persica induced translocation of NF-${\kappa}B$ to the nucleus from the cytosol and DNA-binding activity of nuclear NF-${\kappa}B$ in LPS-activated RAW264.7 cells. The extract mixture of Paeonia Suffruticosa and Prunus Persica showed more significant suppression of the NF-${\kappa}B$ translocation and its DNA-binding activity, as compared to those of the each extract. These results suggest that the extract mixture of Paeonia Suffruticosa and Prunus Persica may affect different control mechanisms for NF-${\kappa}B$ activation and the expression and production of NF-${\kappa}B$-dependent inflammatory mediators, indicating that this extract mixture may be useful for treatment of inflammatory diseases.

Keywords

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