Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
권호 표지

Anti-inflammatory activities of Sagunja-tang(Sijunzi-tang)

사군자탕(四君子湯)의 항염증 효능 연구

  • Lee, Jin-Ah (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Jung, Da-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Ho-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Nam-Hun (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Jun-Kyoung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, Jung-Hoon (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Mee-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
  • 이진아 (한국한의학연구원 한약 EBM 연구센터) ;
  • 하혜경 (한국한의학연구원 한약 EBM 연구센터) ;
  • 정다영 (한국한의학연구원 한약 EBM 연구센터) ;
  • 이호영 (한국한의학연구원 한약 EBM 연구센터) ;
  • 이남헌 (한국한의학연구원 한약 EBM 연구센터) ;
  • 이준경 (한국한의학연구원 한약 EBM 연구센터) ;
  • 서창섭 (한국한의학연구원 한약 EBM 연구센터) ;
  • 김정훈 (한국한의학연구원 한약 EBM 연구센터) ;
  • 이미영 (한국한의학연구원 한약 EBM 연구센터) ;
  • 신현규 (한국한의학연구원 한약 EBM 연구센터)
  • Received : 2010.02.07
  • Accepted : 2010.06.10
  • Published : 2010.06.30
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Abstract

Objectives : To provide the information of efficacy for Sagunja-tang (Sijunzi-tang; SG), it was evaluated the anti-inflammatory effect. SG, a widely used herbal formula in tranditional Korean medicine, has been used to treat for the Boki-invigorating. In many studies, plant-derived anti-inflammatory efficacies have been investigated for their potential inhibitory effects on lipopolysaccharide (LPS)-stimulated macrophages. This study was performed to examine the anti-inflammatory effects of SG extract on LPS-stimulated RAW 264.7 cells. Methods : The productions of nitric oxide (NO), prostaglandin (PG)$E_2$, interleukin (IL)-6 and tumor necrosis factor (TNF)-$\alpha$ were examined in a macrophage cell line, RAW 264.7 cells, in the presence of the SG extract. RAW 264.7 cells were incubated with LPS $1\;{\mu}g/mL$ and SG extract for 18 hours. The anti-inflammatory activity of SG was investigated by carrageenin-induced paw edema in rats. The paw volume was measured at 0, 2 and 4 hours following carrageenin-induced paw edema in rats. Results : SG extract showed inhibitory effect on $PGE_2$, IL-6 and TNF-$\alpha$ by LPS-stimulated RAW 264.7 cells. But SG extract was not inhibitory effect on NO by LPS-stimulated RAW 264.7 cells. And administration of SG extract (1 g/kg) showed a reduction in carrageenin-induced paw edema on rats. Conclusions : These results suggest that SG extract has anti-inflammatory activities in vitro and in vivo models.

Keywords

References

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