Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Study of Inhibitory Effect of Melanogenesis and Antioxidant Activity of Agrimonia pilosa Ledeb

선학초 추출물의 멜라닌합성 억제 및 항산화효과

  • Kim, Dae-Sung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim, Yeong-Mok (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Mun, Yeun-Ja (Department of Anatomy, College of Oriental Medicine, Wonkwang University)
  • 김대성 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 김영목 (원광대학교 한의과대학 병리학교실) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 문연자 (원광대학교 한의과대학 해부학교실)
  • Received : 2010.02.18
  • Accepted : 2010.04.07
  • Published : 2010.04.25
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Abstract

The purpose of this study was to investigate the mechanism of ethanol extract of Agrimonia pilosa Ledeb. (EAP)-reduced melanogenesis and diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity. Agrimonia pilosa Ledeb., a perennial herbaceous plant, has been used as an antihemorrhagic, anthelminntic, and antiinflammatory agents in Chinese herbal medicine. In the present study, we observed that melanin synthesis and tyrosinase activity of B16F10 cells were significantly decreased by EAP. However, EAP could not suppress tyrosinase activity in the cell-free system, whereas kojic acid directly inhibited tyrosinase activity. Furthermore, EAP decreased the protein expression of tyrosinase, tyrosinase-related prootein 1 (TRP-1), and tyrosinase-related prootein 2 (TRP-2). EAP scavenged DPPH radical up to 41% with 100 ${\mu}g/m{\ell}$ concentration. These results suggest that the hypopigmentary effect of EPA was due to regulation of tyrosinase protein.

Keywords

References

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