Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Seroprevalence of Brucellosis and Isolation of Yersinia enterocolitica O:9 in Pigs

돼지에서 브루셀라병 항체조사 및 Yersinia enterocolitica O:9의 분리

  • Jung, Byeong-Yeal (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Byun, Jae-Won (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Kim, Ha-Young (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Shin, Dong-Ho (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Park, Choi-Kyu (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Jung, Suk-Chan (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Service)
  • 정병열 (국립수의과학검역원 질병진단센터) ;
  • 변재원 (국립수의과학검역원 질병진단센터) ;
  • 김하영 (국립수의과학검역원 질병진단센터) ;
  • 신동호 (국립수의과학검역원 질병진단센터) ;
  • 박최규 (국립수의과학검역원 질병진단센터) ;
  • 정석찬 (국립수의과학검역원 세균과)
  • Received : 2010.01.13
  • Accepted : 2010.03.03
  • Published : 2010.05.31
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Abstract

Ten farrow-finish farms participated in this seromonitoring that was conducted to investigate the porcine brucellosis situation in Korea. In total, eight (80.0%) of the 10 farms and 139 (24.0%) of 578 pigs tested showed a positive response in the Rose Bengal test (RBT). Seroprevalence levels were determined using RBT according to age; 35 (14.6%) of 239 piglets, 36 (31.3%) of 115 growing pigs, and 68 (30.4%) of 224 finishing pigs and sows were positive, respectively. All positive samples in RBT were tested with the tube agglutination test (TAT) and competitive ELISA (C-ELISA), simultaneously. Although 48 samples came up positive in the TAT, all samples tested with C-ELISA were negative. Among 26 rectal swab samples from the TAT positive-pigs, Yersinia enterocolitica O:9 was isolated from seven samples (26.9%). Therefore, we speculated that the positive reaction of RBT and TAT in this study might be induced by the serologically cross-reacting bacteria with Brucella abortus.

2007년 전국 10개의 일반 양돈장에서 다양한 일령의 돼지 578두를 채혈하여 Rose Bengal test 진단액으로 스크리닝을 하고, 양성 혈청에 대해서는 시험관응집반응과 C-ELISA를 적용하여 브루셀라병 항체 분포도 양상과 브루셀라 교차반응 유발균의 분리를 시도하여 다음과 같은 결론을 얻었다. 돼지에서 브루셀라병 Rose Bengal 스크리닝 결과, 10주령 미만(양성률; 14.6%, 95% CI; 10.1-19.1)자돈보다 10주령에서 17주령 미만(양성률; 31.3%, 95% CI; 22.8-39.8)과 17주령 이상(양성률; 30.4%, 95% CI; 34.3-36.4)의 돼지에서 양성률이 유의성 있게 높았다(p<0.05). 따라서 검사돈 중 139두 (24.0%)에서 Rose Bengal test 양성반응이 나타났다. 양돈장별 Rose Bengal test 양성률 조사에서 6개 양돈장이 10% 미만의 양성률을 보였고, 2개 양돈장에서는 70% 이상의 높은 양성률이 관찰되었다. Rose Bengal test 양성 혈청 139건을 시험관응집반응과 C-ELISA에 적용한 결과, 시험관응집반응에서 100배 이상의 양성이 48건(34.5%)이었으나 C-ELISA에서는 전 두수 음성으로 나타나 돼지 혈청에 대해서는 시험관응집반응 성적과 C-ELISA 성적에 많은 차이가 있었다. 시험관응집반응에서 높은 항체가를 보인 돼지의 직장 swab 시료(n=26)에서 브루셀라 균과 교차반응을 유발하는 균종에 대해 균분리를 실시한 결과 E. coli O157:H7, Salmonella N group은 분리되지 않았으나 Y. enterocolitica O:9은 7건(26.9%)이 분리되었다.

Keywords

References

  1. Animal Infectious Disease Data Management System, http://aims.nvrqs.go.kr/sta/aimsStat.do. Accessed January 25, 2009.
  2. Anonymous. 2008. Porcine brucellosis. 6th ed. pp. 1108-1114, Manual of diagnostic tests and vaccines for terrestrial animals, OIE, France.
  3. Burriel, A. R., L. Varoudis, C, Alexopoulos, S, Kritas, and S. C. Kyriakis. 2003. Serological evidence of Brucella species and Leptospira interrogans serovars in Greek swine herds. J. Swine Health Prod. 11, 186-189.
  4. Cetinkaya, Z., O. C. Aktepe, I. H. Ciftci, and R. Demirel. 2005. Seroprevalence of human brucellosis in a rural area of western anatolia, Turkey. J. Health Popul. Nutr. 23, 137- 141.
  5. Corbel, M. J. 1985. Effect of atrophic rhinitis vaccines on the reaction of pigs to serological tests for brucellosis. Vet. Rec. 117, 150. https://doi.org/10.1136/vr.117.7.150
  6. Deyoe, B. L. 1967. Pathogenesis of three strains of Brucella suis in swine. Am. J. Vet. Res. 28, 951-957.
  7. Ferris, R. A., M. A. Schoenbaum, and R. P. Crawford. 1995. Comparison of serologic tests and bacteriologic culture for detection of brucellosis in swine from naturally infected herds. J. Am. Vet. Med. Assoc. 207, 1332-1333.
  8. Fukushima, H., K. Saito, M. Tsubokura, K. Otsuki, and Y. Kawaoka. 1983. Isolation of Yersinia spp. from bovine feces. J. Clin. Microbiol. 18, 981-982.
  9. Gall, D. and K. Nielsen. 2004. Serological diagnosis of bovine brucellosis: a review of test performance and cost comparison. Rev. Sci. Tech. Off. Int. Epiz. 23, 989-1002.
  10. Jung, B. Y., S. C. Jung, N. K. Lee, S. K. Cho, D. H. Cho, M. Her, Y. D. Yoon, and B. H. Kim. 1999. Modified sorbitol MacConkey agar for the rapid isolation of Escherichia coli O157:H7. Korean J. Vet. Res. 39, 765-771.
  11. Jung, B. Y., W. I. Kim, K. H. Cho, J. H. Park, and B. H. Kim. 2000. Isolation and genotyping of shiga-like toxin producing Escherichia coli O157 from Korean swine. Proceedings of 16th International Pig Veterinarian Society, Melbourne, Australia.
  12. Jung, B. Y., W. W. Lee, H. S. Lee, O. K. Kim, and B. H. Kim. 2001. Prevalence of Salmonella in slaughter pigs in south Korea. Proceedings of 4th International Symposium on Epidemiology and Control of Salmonella and other Foodborne pathogens in pork. Leipzig, Germany.
  13. Jung, B. Y., K. M. Park, and B. H. Kim. 2004. Antimicrobial susceptibility patterns of Salmonella typhimurium isolated from diseased pigs. Kor. J. Vet. Publ. Hlth. 28, 109-115.
  14. Kubuafor, D. K., B. Awumbila, and B. D. Akanmori. 2000. Seroprevalence of brucellosis in cattle and humans in the Akwapim-South district of Ghana: public health implications. Acta. Tropica. 76, 45-48. https://doi.org/10.1016/S0001-706X(00)00088-7
  15. Lord, V. R., J. W. Cherwonogrodzky, M. J. Marcano, and G. Melendez. 1997. Serological and bacteriological study of swine brucellosis. J. Clin. Microbiol. 35, 295-297.
  16. Lubeck, P. S., M. Skurnik, P. Ahrens, and J. Hoorfar. A multiplex PCR-detection assay for Yersinia enterocolitica serotype O:9 and Brucella spp. based on the perosamine synthetase gene. http://www.hi.helsinki.fi/yersinia/pdf_own/Lubeck_AdExpMeBio_B_2003.pdf. Accessed August 6, 2007.
  17. MacMillan, A. P. 1999. Brucellosis. pp. 385-393, In Straw, B. E., S. D'Allaire, W. L. Mengeling, and D. J. Taylor (eds.), Diseases of Swine. 8th eds. Iowa State University Press, Iowa.
  18. Al-Majali, A. M., A. Q. Talafha, M. M. Ababneh, and M. M. Ababneh. 2009. Seroprevalence and risk factors for bovine brucellosis in Jordan. J. Vet. Sci. 10, 61-65. https://doi.org/10.4142/jvs.2009.10.1.61
  19. McNally, A., T. Cheasty, C. Fearnley, R. W. Dalziel, G. A. Paiba, G. Manning, and D. G. Newell. 2004. Comparison of the biotypes of Yersinia enterocolitica isolated from pigs, cattle and sheep at slaughter and from humans with yersiniosis in Great Britain during 1999-2000. Letters Appl. Microbiol. 39, 103-108. https://doi.org/10.1111/j.1472-765X.2004.01548.x
  20. Ministry for Food, Agriculture, Forestry and Fisheries. http://law.go.kr/LSW/AdmRulInfoP.do?admRulSeq=77138&admFlag=0. Accessed October 5, 2009.
  21. Ministry for Food, Agriculture, Forestry and Fisheries. http://law.go.kr/LSW/Adm RulInfo P.do?admRulSeq=77211&admFlag=0. Accessed October 5, 2009.
  22. Munoz, P. M., C. M. Marin, D. Monreal, D. Gonzalez, B. Garin-Bastuji, R. Diaz, R. C. Mainar-Jaime, I. Moriyon, and J. M. Blasco. 2005. Efficacy of several serological tests and antigens for diagnosis of bovine brucellosis in the presence of false-positive serological results due to Yersinia enterocolitica O:9. Clin. Diagn. Lab. Immunol. 12, 141-151.
  23. Nielsen, K. 2002. Diagnosis of brucellosis by serology. Vet. Microbiol. 90, 447-459. https://doi.org/10.1016/S0378-1135(02)00229-8
  24. Park, S. G., C. S. Choi, and Y. S. Jeon. 1992. Biotype, serotype and antibiotic susceptibility of Yersinia enterocolitica isolated from swine. Korean J. Vet. Res. 32, 63-76.
  25. Park, S. G., S. M. Choi, Y. H. Oh, and C. S. Choi. 1993. Biotype, serotype and antimicrobial susceptibility of Yersinia enterocolitica isolated from cattle. J. Korean Soc. Microbiol. 28, 453-461.
  26. Park, S. G., E. S. Youn, and E. J. Kim. 1994. Biotype, serotype and antibiotic susceptibility of Yersinia enterocolitica isolated from zoo animals. Korean J. Vet. Res. 34, 85-91.
  27. Paulo, P. S., A. M. Vigliocco, R. F. Ramondino, D. Marticorena, E. Bissi, G. Briones, C. Gorchs, D. Gall, and K. Nielsen. 2000. Evaluation of primary binding assays for presumptive serodiagnosis of swine brucellosis in Argentina. Clin. Diagn. Lab. Immunol. 7, 828-831.
  28. Rogers, R. J., D. R. Cook, P. J. Ketterer, F. C. Baldock, P. J. Blackall, and R. W. Stewart. 1989. An evaluation of three serological tests for antibody to Brucella suis in pigs. Aust. Vet. J. 66, 77-80. https://doi.org/10.1111/j.1751-0813.1989.tb09748.x
  29. Sung, K. C. and W. P. Choi. 1987. Characterization of Yersinia species isolated from animals in Korea. Korean J. Vet. Res. 27, 235-243.
  30. Thibodeau, V., E. H. Frost, S. Chenier, and S. Quessy. 1999. Presence of Yersinia enterocolitica in tissues of orally-inoculated pigs and the tonsils and feces of pigs at slaughter. Can. J. Vet. Res. 63, 96-100.
  31. van der Giessen, J. W. and A. Priadi. 1988. Swine brucellosis in Indonesia. Vet. Q. 10, 172-176. https://doi.org/10.1080/01652176.1988.9694167
  32. Weagant, S. D., P. Feng, and J. T. Stanfield. 1992. Yersinia enterocolitica and Yersinia pseudotubersulosis. Bacteriological analytical manual. pp. 95-109, 7th eds. AOAC International, USA.
  33. World Animal Health Information Database-Version 1.2. http://www.oie.int/wahis/public.php?page=disease_ immediate_summary&disease_type=Terrestrial&disease_id=186&empty=999999. Accessed Feburary 12, 2009.