Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Influence of Pinewood Nematode, Bursaphelenchus xylophilus, on the Growth of Endoparasitic Fungus Esteya vermicola

Endoparasitic fungus Esteya vermic의 성장에 미치는 소나무 선충 Bursaphelenchus xylophilus의 영향

  • Wang, Chun-Yan (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University) ;
  • Lee, Chung-Ha (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University) ;
  • Lee, Mi-Ra (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University) ;
  • Yun, Beom-Sik (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University) ;
  • Liu, Lei (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University) ;
  • Wang, Zhen (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University) ;
  • Fang, Zhe-Ming (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University) ;
  • Zhang, Dong-Liang (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University) ;
  • Li, Zheng (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University) ;
  • Sung, Chang-Keun (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University)
  • 왕춘연 (충남대학교 농업생명공학관 식품공학과) ;
  • 이청하 (충남대학교 농업생명공학관 식품공학과) ;
  • 이미라 (충남대학교 농업생명공학관 식품공학과) ;
  • 윤범식 (충남대학교 농업생명공학관 식품공학과) ;
  • 유뢰 (충남대학교 농업생명공학관 식품공학과) ;
  • 왕젠 (충남대학교 농업생명공학관 식품공학과) ;
  • 방철명 (충남대학교 농업생명공학관 식품공학과) ;
  • 장동량 (충남대학교 농업생명공학관 식품공학과) ;
  • 이정 (충남대학교 농업생명공학관 식품공학과) ;
  • 성창근 (충남대학교 농업생명공학관 식품공학과)
  • Received : 2009.10.20
  • Accepted : 2010.04.28
  • Published : 2010.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

The influence of nematodes on nematophagous fungi has seldom been investigated. In the present study, the influence of pinewood nematode on its endoparasitic fungus, Esteya vermicola, was investigated systemically. Although both nematodal metabolite and nematodal homogenate could stimulate and speed up the growth of E. vermicola, the impact of nematodal metabolite was slightly higher than that of nematodal homogenate. In addition, a method was developed to investigate the influence of volatiles, discharged by pinewood nematodes in their metabolic process, on the growth of E. vermicola. Reproductive results were given and confirmed that nematodal volatiles have no influence on the cell growth of E. vermicola. This study may provide information for the application of E. vermicola as biological control agent of pinewood nematode.

본 논문은 선충 포식성 곰팡이에 대한 선충의 영향을 조사한 연구로, 소나무 기생곰팡이인 E. vermicola에 미치는 소나무 선충의 영향을 체계적으로 분석하였다. 선충 대사물과 선충 균질액 모두 E. vermicola의 성장을 빠르게 촉진하였으나, 선충 대사물이 선충 균질액보다 약간 높은 효과를 보였다. 또한 소나무 선충이 생성하는 휘발성 물질이 E. vermicola 성장에 미치는 영향을 분석하는 새로운 실험방법을 개발하였다. 본 연구결과에 따르면, 선충의 휘발성 물질은 E. vermicola에 아무런 영향을 끼치지 않는다는 재현성 있는 결과를 도출하였다. 본 연구는 소나무 선충의 생물학적인 조절인자로서 E. vermicola을 적용할 수 있는 정보를 제공할 수 있을 것이다.

Keywords

References

  1. Balan, J., L. Krizkova, P. Nemec, and A. Kolozsvary. 1976. A qualitative method for detection of nematode attracting substances and proof of production of three different attractants by the fungus Monacrosporium rutgeriensis. Nematologica. 22, 306-311. https://doi.org/10.1163/187529276X00580
  2. Field, J. J. and J. Webster. 1977. Traps of predacious fungi attract nematodes. Trans. Brit. Mycol. Soc. 68, 467-469. https://doi.org/10.1016/S0007-1536(77)80210-6
  3. Han, H., Y. J. Chung, and S. C. Shin. 2008. First Report of Pine Wilt Disease on Pinus koraiensis in Korea. Plant Dis. 92, 1251.
  4. Jasson, H. B. 1982. Attraction of nematodes to endoparasitic nematophagous fungi. Trans. Brit. Mycol. Soc. 79, 25-29. https://doi.org/10.1016/S0007-1536(82)80187-3
  5. Jasson, H. B. and B. Nordbring-Hertz. 1979. Attraction of nematodes to living mycelium of nematophagous fungi. J. Gen. Microbiol. 112, 89-93. https://doi.org/10.1099/00221287-112-1-89
  6. Jasson, H. B. and B. Nordbring-Hertz. 1980. Interactions between nematophagous fungi and plant-parasitic nematode: attraction, induction of trap formation and capture. Nematologica. 26, 383-389. https://doi.org/10.1163/187529280X00323
  7. Liou, J. Y., J. Y. Shih, and S. S. Tzean. 1999. Esteya, a new nematophagous genus from Taiwan, attacking the pinewood nematode (Bursaphelenchus xylophilus). Mycol. Res. 103, 242-248. https://doi.org/10.1017/S0953756298006984
  8. Mamiya, Y. 2006. Attraction of the pinewood nematode to mycelium of some wood-decay fungi. Jpn. J. Nematol. 36, 1-9. https://doi.org/10.3725/jjn.36.1
  9. Monoson, H. L., A. G. Galsky, J. A. Griffin, and E. J. Mcgrath. 1973. Evidence for and partial characterization of a nematode attraction substance. Mycol. 65, 78-86. https://doi.org/10.2307/3757788
  10. Nordbring-Hertz, B. and G. Odham. 1980. Determination of volatile nematode exudates and their effects on a nematode- trapping fungus. Microb. Ecol. 6, 241-251. https://doi.org/10.1007/BF02010389
  11. Schenck, S. and D. Pramer. 1975. The effects of volatile compounds from nematodes on trap formation by a nematode- trapping fungus. Appl. Microbiol. 30, 496-497.
  12. Tzean, S. S., J. Y. Liou, and J. Y. Shih. 2001. Nematophagous fungus Esteya vermicola, United States Patent 006168947B1.
  13. Wang, C. Y., Z. M. Fang, B. S. Sun, L. J. Gu, K. Q. Zhang, and C. K. Sung. 2008. High infectivity of an endoparasitic fungus strain, Esteya vermicola, against nematodes. J. Microbiol. 46, 380-389. https://doi.org/10.1007/s12275-007-0122-7
  14. Wang, C. Y., Z. M. Fang, Z. Wang, L. J. Gu, B. S. Sun, D. L. Zhang, and C. K. Sung. 2009. High infection activities of two Esteya vermicola isolates against pinewood nematode. Afr. J. Microbiol. Res. 3, 581-584.
  15. Yi, C., B. Byun, J. Park, S. Yang, and K. Chang. 1989. First finding of the pine wood nematode, Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle and its insect vector in Korea. Res. Rep. For. Res. Inst. Seoul 38, 141-149.

Cited by

  1. Research advance on Esteya vermicola, a high potential biocontrol agent of pine wilt disease vol.14, pp.12, 2015, https://doi.org/10.1007/s11557-015-1137-9