Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Effect of Aster glehni Fr. Schm. Extracts on Whitening and Anti-Wrinkle

섬쑥부쟁이(Aster glehni Fr. Schm.) 분획물의 미백 및 주름 개선 효과

  • Kim, Han-Hyuk (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Park, Gun-Hye (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Park, Kang-Soo (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Kim, Tae-Hoon (Department of Herbal Medicinal Pharmacology, Daegu Haany University) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University)
  • 김한혁 (대구한의대학교 화장품약리학과) ;
  • 박근혜 (대구한의대학교 화장품약리학과) ;
  • 박강수 (대구한의대학교 화장품약리학과) ;
  • 이진영 (호서대학교 한방화장품과학과) ;
  • 김태훈 (대구한의대학교 한약재약리학과) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Received : 2010.03.17
  • Accepted : 2010.07.21
  • Published : 2010.07.30
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Abstract

Plants and their extracts can be utilized as inexpensive and rich resources of active constituents in the cosmetic field, as well as the food, pharmaceutical and medicinal fields. Until now, Aster glehni Fr. Schm. had no known active effect, except on anti-oxidation, that was found during investigations for application in the cosmetic field. In this study, we examined the inhibition of enzymatic reactions to protein levels in inclusive B16F10 melanoma cell lines. Significant inhibition of enzymatic reactions was observed in the EtOAc extract, which advanced to tyrosinase protein and TRP-1 in the B16F10 melanoma cell line. These results indicated that the effect of EtOAc extract inhibited expressions of tyrosinase protein and TRP-1 in the B16F10 melanoma cell line by 30.5% and 41.5% at 100ug/ml respectively. On the other hand, antimicrobial activity was evaluated to the four fractions in normal flora of the skin. Hexane extract was only exhibited in the higher clear zone in all strains. In conclusion, any cosmeceutical effects of Aster glehni Fr. Schm. may have a potential meaning, as well as possible value for further studies regarding the effects of chemical constituents of Aster glehni Fr. Schm.

천연의 식물과 추출물은 비용의 효율성과 풍부한 자원을 이점으로 식품, 약, 의약 분야와 마찬가지로 화장품 분야에서도 이용될 수 있다. 현재까지 섬쑥부쟁이는 항산화 활성을 제외하고는 그 효능에 대해 알려진 바가 없다. 따라서 본 연구에서는 울릉도의 대표적인 특산 식물인 섬쑥부쟁이 추출물을 이용하여 화장품 분야에서의 활용방안에 대한 연구를 조사한바, 효소적 in vitro 검색법을 측정하여 이들 중 가장 우수한 EtOAc 추출물을 대상으로 이러한 저해능이 멜라닌 생성에 관련된 단백질 발현과도 연관성이 있는지를 확인하기 위해 B16F10 melanoma cell line 내 단백질 수준을 확인하였다. Tyrosinase, TRP-1의 항체를 이용한 western blot으로 관련 단백질의 발현량을 조사한 결과 섬쑥부쟁이 에틸아세테이트 분획물 100 ug/ml을 처리한 군에서는 tyrosinase protein을 30.5%를 억제하였고, TRP-1은 41.5% 억제하였다. 한편, 섬쑥부쟁이 분획물의 생육 저해환 측정에서는 Staphylococcus epidermidis, Propionibacterium acnes, Escherichia coli에 대하여 헥산 분획물 2 mg/disc에서 각각 21.0 mm, 12.0 mm, 14.0 mm의 저해환을 나타내어 섬쑥부쟁이 헥산 분획물만이 유일하게 높은 항균활성을 나타났다. 이들 실험 결과를 미루어보아, 섬쑥부쟁이의 화장품 분야에서의 활용적 가치가 충분하다고 사료되나, 그 활성 성분에 대한 명확한 규명을 위한 추가적인 연구를 제시하는 바이다.

Keywords

References

  1. An, B. J., C. E. Lee, J. H. Son, J. Y. Lee, G. H. Choi, and T. S. Park. 2005. Antioxidant, anticancer and tyrosinase inhibition activities of extracts from Rhododendron mucronulatum T. J. Korean Soc. Appl. Biol. Chem. 48, 280-284.
  2. Caccioni, D. R. L., M. Guizzardi, D. M. Biondi, A. Renda, and G. Ruberto. 1998. Relationship between volatile components of citrus fruit essential oils and antimicrobial action on Penicillium digitatum and Penicillium italicym. International J. of Food Microbiol. 43, 73-79. https://doi.org/10.1016/S0168-1605(98)00099-3
  3. Cannell, R. J. P., S. J. Kellan, A. M. Owsianks, and J. M. Walker. 1988. Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Med. 54, 10-14. https://doi.org/10.1055/s-2006-962319
  4. Carmichael, J., W. G. DeGraff, A. F. Gazdar, J. D. Minna, and J. B. Mitchell. 1987. Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47, 936-942.
  5. Cho, M. J. 2001. A study on the antioxidant nutrients analysis and antioxidative activities of A denophora remotif lora and Aster glehni. MS Thesis, Duksung Women's University Seoul, Korea.
  6. Choi, M. G., H. S. Chung, and K. D. Moon. 2008. Chemical components of Solidago virgaurea spp. gigantea, Aster glehni var. hondoensis and Aruncus dioicus var. kamtschaticus grown on Ulleung Island. Korean J. Food Preserv. 15, 576-581.
  7. Conner, D. E. and L. R. Beuchat. 1984. Sensitivity of heat-stressed yeasts to essential oils of plants. Appl. Environ. Microbiol. 47, 229-233.
  8. Demina, N. S. and S. V. Lysenko. 1996. Collagenolytic enzymes synthesized by microorganisms. Mikrobiologiia 65, 293-304.
  9. Goldberg, I. 1994. Functional Foods. pp. 3-550, Chapman & Hall Press. New York, NY, USA.
  10. Grant, N. H., and H. E. Alburn. 1959. Studies on the collagenases of Clostridium histolyticum. Arch. Biochem. Biophys. 82, 245-255. https://doi.org/10.1016/0003-9861(59)90120-1
  11. Han, K. S., S. S. Ham, E. H. Jeong, and H. K. Lee. 1992. Antimutagenic effects of the edible mountain herb juices against Trp-P-1 and 2AF. J. Fd. Hyg. Safty 7, 161-168.
  12. Im, R. J. and B. S. Do. 1988. Plant Book. pp. 672, Social Science Publishers Seoul. Korea.
  13. Kaba, T., K. Morita, and T. Inoue. 1978. Antimutagenic action of vegetable factor on the mutagenic principle of tryptophane pyrolysate. Mutation Res. 53, 351-353. https://doi.org/10.1016/0165-1161(78)90008-0
  14. Kang, K. S. 2007. The evaluation of anti-wrinkle effects in oriental herb extract. J. Life Science. 17, 1147-1151. https://doi.org/10.5352/JLS.2007.17.8.1147
  15. Kang, Y. C., K. K. Choi, K. H. Kim, and H. K. Kim. 2002. Microencapsulation of aster scaber and Aster glehni by spray drying. Korean J. Food Preserv. 9, 212-220.
  16. Kim, C. M., M. K. Sin, T. K. An, and K. S. Lee. 1997. Dictionary of Chinese Herb, pp. 1431, JucngDam Publisher, Seoul, Korea.
  17. Kim, H. K., Y. J. Kwon, K. H. Kim, and Y. H. Jeong. 2000. Changes of total polyphenol content and electron donating ability of Aster glehni extracts with different microwave-assisted extraction conditions. Korean J. Food Sci. Technol. 32, 1022-1028.
  18. Kim, K. W., J. G. Shin, and D. G. Lee. 2008. Isolation of herbicidal compound from Aster glehni Fr. Schm. Kor. Soc. Weed Sci. 28, 434-441.
  19. Kwon, Y. J., K. H. Kim, and H. K. Kim. 2002. Changes of total polyphenol content and antioxidant activity of Ligularia fisheri extracts with different microwave-assisted extraction conditions. Korean J. Food Preserv. 9, 332-337.
  20. Lee, C. B. 2006. Coloured flora of Korea. pp. 322, Hyang moon sa, Seoul, Korea.
  21. Lee, M. S. and M. S. Jung. 1998. Analysis of volatile flavor components of Aster grehni. Korean J. Soc. Food Sci. 14, 547-552.
  22. Lee, J. S. 2000. Effects of NAA on stem cuttings of varigated Aster glehni, Achillea sibirica and Solidago virga-aurea var. asiatica native to Korea. J. Korean Env Res & Reveg Tech. 3, 8-11.
  23. Min, Y. D., H. C. Kwon, S. Z. Choi, and K. R. Lee. 2004. Terpenoids from the aerial parts of Aster grehni. Yakhak Hoeji. 48, 65-69.
  24. Nam, Y. and J. Baik. 2005. Status of research and possibility of development about endemic wild vegetables in Korea. J. Korean Soc. Plants People Environment. 8, 1-10.
  25. Sadaki, O. 1996. The development of functional foods and materials. Bioindustry 13, 44-50.
  26. Sim, G. S., J. H. Kim, B. C. Lee, D. H. Lee, G. S. Lee, and H. B. Pyo. 2008. Inhibitory Effects on Melanin Production in B16 Melanoma Cells of Sedum sarmentosum. Yakhak Hoeji. 52, 165-171.
  27. Voegeli, R. 1996. Elastase and tryptase determination on human skin surface. Cosmetic & Toiletries 111, 51-58.
  28. Wunsch, E. and H. G. Heindrich. 1963. Zur quantitativen bestimmung der collagenase. Hoppe-Seyler's Physiol. Chem. 333, 149-151. https://doi.org/10.1515/bchm2.1963.333.1.149
  29. Yagi, A., T. Kanbara, and N. Morinobu. 1986. The effect of tyrosinase inhibition for aloe. Planta Medica. 3981, 517-519.
  30. Yoon, J. K., D. H. Lee, N. M. Kim, and J. S. Lee. 2005. Screening and extraction condition of anti-skin aging elastase inhibitor from medicinal plant. Korean J. Medicinal Crop. Sci. 13, 213-216.

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