Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Genetic Diversity and Relationship of Genus Spiraea by Random Amplified Polymorphic DNA Markers

조팝나무속 분류군의 RAPD에 의한 유전적 다양성과 관련성

  • Huh, Man-Kyu (Department of Molecular Biology, Dongeui University)
  • 허만규 (동의대학교 분자생물학과)
  • Received : 2010.04.06
  • Accepted : 2010.04.29
  • Published : 2010.07.30
Download PDF
⟨ Previous Next ⟩

Abstract

Genus Spiraea is a woody species primarily distributed throughout Asia. Many species of this genus are important plants medicinally and ecologically. I evaluated a representative sample of the sixteen taxa with random amplified polymorphic DNA (RAPD) markers to estimate genetic relationships within genus Spiraea. In addition, RAPD analysis was also conducted to estimate the genetic diversity and population structure of these species. As the typical populations of Spiraea were small, isolated, and patchily distributed for natural populations, they maintained a low level of genetic diversity for polymorphic primers. The mean H was 0.117 across species. The Korean endemic species (S. chartacea) and patchily distributed species (S. betulifolia) showed fewer alleles per locus (mean 1.240 vs. 1.297), lower percent polymorphic locus (24.0 vs. 29.7), and lower diversity (0.092 vs. 0.121) than a relatively widely spread species. An assessment of the proportion of diversity present within species, $H_{POP}/H_{SP}$, indicated that about 87.8% the total genetic diversity was among species. Thus, the majority of genetic variation (87.8%) resided within species. The phylogenic tree showed three distinct groups. One clade includes S. prunifolia for. simpliciflora, S. thunbergii, S. chamaedryfolia var. ulmifolia, S. media, and S. cantoniensis. Another clade includes S. blumei, S. pubescens, S. chartacea, and S. chinensis. The other clade is the remaining seven species.

조팝나무속 식물은 주로 아시아에 많이 분포하는 목본으로 생태 및 약용으로 중요하다. 이 속내 16종, 29집단에 대해 RAPD (random amplified polymorphic DNA) 마커로 이들 집단에 대한 유전적 변이와 집단구조를 조사 하였다. 이들 집단은 작고 격리되어 있어 낮은 유전적 다형성을 나타내었다. 전체 유전적 다양도는 종 수준에서 0.117이였다. 국지적 분포를 보이는 종(S. chartacea)은 광범위하게 분포하는 종에 비해 유전자 좌위당 대립유전자의 수는 적었고(평균 1.240:1.297), 다형성을 나타내는 유전자 좌위 %(24.0:29.7), 낮은 다형성(0.092 vs. 0.121)을 나타내었다. 종내 다양성의 비율($H_{POP}/H_{SP}$)은 전체 변이중 87.8%가 종간에 있었고 전체 변이의 12.2%는 종내에 있었다. 계통도에서 세 그룹으로 나타났다. 한 분지군은 조팝나무, 가는잎조팝나무, 인가목조팝나무, 긴조팝나무, 공조팝나무이었다. 또한 분지군은 산조팝나무, 아구장나무, 떡잎조팝나무, 당조팝나무였다. 나머지 분지군은 7종을 포함하고 있었다.

Keywords

References

  1. Barrett, S. C. H., C. G. Eckert, and B. C. Husband. 1993. Evolutionary processes in aquatic plant populations. Aquatic Botany 44, 105-145. https://doi.org/10.1016/0304-3770(93)90068-8
  2. Bowman, K. D., K. Hutcheson, E. P. Odum, and L. R. Shenton. 1971. Comments on the distribution of indices of diversity. Stat. Ecol. 3, 315-359.
  3. Bussell, L. D. 1999. The distribution of random amplified polymorphic DNA (RAPD) diversity among populations of Isotomapetraea (Lobeliaceae). Mol. Ecol. 8, 775-789. https://doi.org/10.1046/j.1365-294X.1999.00627.x
  4. Collard, B. C. Y. and D. J. Mackill. 2009. Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Mol. Biol. Rep. 27, 86-93. https://doi.org/10.1007/s11105-008-0060-5
  5. Felsenstein, J. 1989. PHYLIP (Phylogeny Inference Package) version 3.2. Cladistics 5, 164-166.
  6. Hamrick, J. L. and M. J. W. Godt. 1989. Allozyme diversity in plant species, pp. 43-63, In Brown, A. H. D., M. T. Clegg, A. L. Kahler, and B. S. Weir (eds.), Plant population genetics, breeding and genetic resources. Sinauer Associates, Sunderland, MA.
  7. Hamrick, J. L. and M. J. W. Godt. 1996. Effects of life history traits on genetic diversity in plant species, Philoso. Trans. Royal Soc. London Series B 351, 1291-1298. https://doi.org/10.1098/rstb.1996.0112
  8. Kim, T. J. and B. Y. Sun. 1996. Taxonomy of the genus Spiraea in Korea. Kor. J. Plant Tax. 26, 191-212.
  9. Lee, Y. N. 2007. New Flora of Korea. pp. 522-527, Kyo-Hak Publishing Co., Seoul, Korea.
  10. Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70, 3321-3. https://doi.org/10.1073/pnas.70.12.3321
  11. Nei, M. and W. H. Li. 1979. Mathematical model for studying genetical variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 74, 5267-5273.
  12. Paul, S. P., F. N. Wachira, W. Powell, and R. Waugh. 1997. Diversity and genetic differentiation among populations of Indian and Kenyan tea (Camelliasinensis (L.) O. Kuntze) revealed by AFLP markers. Theor. Appl. Genet. 94, 255-263. https://doi.org/10.1007/s001220050408
  13. Potter, D., S. M. Still, T. Grebenc, D. Ballian, G. Bozic, J. Franjiae, and H. Kraigher. 2007. Phylogenetic relationships in tribe Spiraceae (Rosaceae) inferred from nucleotide sequence data. Pl. Syst. Evol. 266, 105-118. https://doi.org/10.1007/s00606-007-0544-z
  14. Rashed, M. A., M. H. Abou-Deif, M. A. A. Sallam, and W. A. Ramadan. 2008. Estimation of genetic diversity among thirty bread wheat varieties by RAPD analysis. J. Appl. Sci. Res. 4, 1898-1905.
  15. Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
  16. Sawalha, K., H. Eideh, S. Laham, H. Hasasneh, and B. Mezeid. 2008. Genetic diversity studies on wheat landraces in Palestine using RAPD markers in comparison to phenotypic classification. J. Appl. Biol. Sci. 2, 29-34.
  17. Thawaro, S. and S. Te-chato. 2008. RAPD (random amplified polymorphic DNA) marker as a tool for hybrid oil palm verification from half mature zygotic embryo culture. J. Agricult. Techno. l4, 165-176.
  18. Woo, M.H., E. H. Lee, O. Chung, and C. W. Kim. 1996. Constituents of Spiraea prunifolia var. simpliciflora. Nat. Prod. Sci. 27, 389-396.
  19. Wright, S. 1965. The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution 19, 395-420. https://doi.org/10.2307/2406450
  20. Yeh, F. C., R. C. Yang, T. J. Botle, Z. H. Ye, and J. X. Mao. 1997. POPGENE, the user friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Canada.
  21. Yu, T. T. and L. T. Yu. 1974. Spiraea, pp. 1-36, In Yu, T. T. (ed.), Flora reipublic popularis sinicae. Angiospermae dicotyledonae. Science Press, Beijing.
  22. Zhang, Z. Y., L. M. Fan, J. B. Yang, X. J. Hao, and Z. J. Gu. 2006. Alkaloid polymorphism and ITS sequence variation in the Spiraea japonica complex (Rosaceae) in China: tracts of the biological effects of the Himalaya-Tibet Plateau uplift. Am. J. Bot. 93, 762-769. https://doi.org/10.3732/ajb.93.5.762
  23. Zhang, Z.Y., H. Sun, and Z. J. Gu. 2002. Karyomorphological study of the Spiraea japonica complex (Rosaceae). Brittonia 54, 168-174. https://doi.org/10.1663/0007-196X(2002)054[0168:KSOTSJ]2.0.CO;2