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생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Zebrafish Dnd protein binds to 3'UTR of geminin mRNA and regulates its expression

  • Chen, Shu (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University) ;
  • Zeng, Mei (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University) ;
  • Sun, Huaqin (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University) ;
  • Deng, Wenqian (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University) ;
  • Lu, Yilu (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University) ;
  • Tao, Dachang (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University) ;
  • Liu, Yunqiang (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University) ;
  • Zhang, Sizhong (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University) ;
  • Ma, Yongxin (Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University)
  • 투고 : 2010.04.29
  • 심사 : 2010.05.01
  • 발행 : 2010.06.30
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초록

Dnd (dead end) gene encodes an RNA binding protein and is specifically expressed in primordial germ cells (PGCs) as a vertebrate-specific component of the germ plasma throughout embryogenesis. By utilizing a technique of specific nucleic acids associated with proteins (SNAAP), 13 potential target mRNAs of zebrafish Dnd (ZDnd) protein were identified from 8-cell embryo, and 8 target mRNAs have been confirmed using an RT-PCR analysis. Of the target mRNAs, the present study is focused on the regulation of geminin, which is an inhibitor of DNA replication. Using electrophoretic mobility shift assay (EMSA), we demonstrated that ZDND protein bound the 67-nucleotide region from 864 to 931 in the 3'UTR of geminin mRNA, a sequence containing 60.29% of uridine. Results from a dual-luciferase assay in HEK293 cells showed that ZDND increases the translation of geminin. Taken together, the identification of target mRNA for ZDnd will be helpful to further explore the biological function of Dnd in zebrafish germ-line development as well as in cancer cells.

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참고문헌

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피인용 문헌

  1. Germ cell-specific expression of dead end (dnd) in rare minnow (Gobiocypris rarus) vol.41, pp.2, 2015, https://doi.org/10.1007/s10695-015-0029-x
  2. Geminin a multi task protein involved in cancer pathophysiology and developmental process: A review vol.131, 2016, https://doi.org/10.1016/j.biochi.2016.09.022
  3. In Vitro Generation of Zebrafish PGC-Like Cells1 vol.91, pp.5, 2014, https://doi.org/10.1095/biolreprod.114.121491
  4. The germ cell marker dead end reveals alternatively spliced transcripts with dissimilar expression vol.9, pp.1, 2019, https://doi.org/10.1038/s41598-019-39101-9