BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Tissue-specific gene expression analysis of silkworm (Bombyx mori) by quantitative real-time RT-PCR

  • Park, Seung-Won (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association) ;
  • Kang, Seok-Woo (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association) ;
  • Goo, Tae-Won (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association) ;
  • Kim, Seong-Ryul (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association) ;
  • Lee, Gwang-Gill (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association) ;
  • Paik, Soon-Young (Department of Microbiology, College of Medicine, The Catholic University of Korea)
  • 투고 : 2009.11.12
  • 심사 : 2010.06.07
  • 발행 : 2010.07.31
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초록

The Bombyx mori Microarray Database (BmMDB; http://silkworm.swu.edu.cn/microarray) provides information for tissue-specific gene expression by using the whole-genome oligonucleotide microarray in the silkworm. We analyzed the tissue-specific expression patterns in the silk gland, fat body, and midgut five days of fifth instar larvae during the development of B. mori. To verify the tissue-specific expression, analysis was conducted using quantitative Real-time RT-PCR and the highly expressed endogenous Actin RNA as an intrinsic reference. Finally, we confirmed five genes, (sw15872, sw00692, sw20990, sw05300,and sw2250), out of 18 candidates expressed in two different tissues, which was consistent with the data published by Dr. Xiang's group, thereby supporting the BmMDB. Further studies for promoter regions of candidate genes can be applied in creating transgenic silkworms as biomedical insects for use in producing biomaterials, and to serve as well-characterized models for understanding the mechanism for the genetic regulation of tissue-specific development.

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참고문헌

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피인용 문헌

  1. Characterization of the Promoter Controling the Stage-Specific Gene Expression of Bombyx mori vol.21, pp.10, 2011, https://doi.org/10.5352/JLS.2011.21.10.1466
  2. Hemocyte-specific Promoter for the Development of Transgenic Silkworm, Bombyx mori vol.25, pp.1, 2012, https://doi.org/10.7852/ijie.2012.25.1.111
  3. Analysis of Hemocyte-specific Gene Expression from Bombyx mori vol.23, pp.1, 2011, https://doi.org/10.7852/ijie.2011.23.1.137
  4. Alternative splicing, expression patterns and promoter characters of vasa-like gene from the silkworm, Bombyx mori vol.39, pp.5, 2012, https://doi.org/10.1007/s11033-011-1409-7
  5. Comparison of gloverin gene expression patterns between domesticated and wild silkworms vol.33, pp.2, 2016, https://doi.org/10.7852/ijie.2016.33.2.113
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