Journal of the Korean Society for Precision Engineering (한국정밀공학회지)

Korean Society for Precision Engineering (한국정밀공학회)
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Evaluation of Hormone Deficiency in Vertebral Body: Analysis of Bone Structure and Quality

호르몬 결핍이 척추체에 미치는 영향 평가: 골의 구조학적 및 질적 요소 분석

  • Kim, Chi-Hoon (Department of Biomedical Engineering and Institute of Medical Engineering, Yonsei Univ.) ;
  • Woo, Dae-Gon (Department of Biomedical Engineering and Institute of Medical Engineering, Yonsei Univ.) ;
  • Park, Ji-Hyung (Department of Biomedical Engineering and Institute of Medical Engineering, Yonsei Univ.) ;
  • Lee, Beob-Yi (Department of Anatomy School of Medicine, Konkuk Univ.) ;
  • Kim, Chi-Hyun (Department of Biomedical Engineering and Institute of Medical Engineering, Yonsei Univ.) ;
  • Kim, Han-Sung (Department of Biomedical Engineering and Institute of Medical Engineering, Yonsei Univ.)
  • Received : 2009.08.24
  • Accepted : 2010.02.24
  • Published : 2010.05.01
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Abstract

This study evaluated the structure and quality of osteoporotic vertebral bone. To induce osteoporosis, eight rats were ovariectomized (OVX). All rats were divided into two groups (Normal group: 4, OVX group: 4). Total lumbar vertebrae for each rat were scanned by in-vivo ${\mu}CT$ at 0, 4 and 8 weeks. Morphological characteristics (BV/TV, Tb.Th, Tb.N, Tb.Sp and SMI) were calculated by in-vivo ${\mu}CT$ image analyzer. Three dimensional finite element models were analyzed to investigate bone strength of OVX and Normal groups. Moreover, the elastic modulus was quantitatively analyzed to evaluate the quality changes of osteoporotic bone. In the OVX group, BV/TV, Tb.Th and Tb.N were significantly decreased at all the lumbar over time (p<0.05). We also investigated a contrary tendency in Tb.Sp and SMI, compared to the above results in each group. A degree of alteration of mechanical characteristics in OVX group was decreased over measuring time (p<0.05). Bone quality presented by distribution of elastic modulus was improved in the Normal group more than OVX group. The findings of the present study indicated that both bone structure and quality of whole lumbar could be tracked and detected by analyzing the morphological and biomechanical characteristics of bones, based on a nondestructive method.

Keywords

References

  1. Liu, S. Z., Yan, H., Xu, P., Li, J. P., Zhuang, G. H., Zhu, B. F. and Lu, S. M., "Correlation Analysis Between Bone Mineral Density and Serum Element Contents of Postmenopausal Women in Xi'an Urban Area," Biol. Trace Elem. Res., Vol. 131, No. 3, pp. 205-214, 2009. https://doi.org/10.1007/s12011-009-8363-4
  2. Kim, S. H. and Kim, K. J., "Effect of Tower Climbing Exercise, Aerobic Exercise & Alpha Lipoic Acid Administration on Bone Metabolism Factor in Ovariectomized," The Korean Journal of Exercise Nutrition, Vol. 13, No. 1, pp. 37-44, 2009.
  3. Lee, M. S., Kang, P. S. and Lee, K. S., "Factors Affecting Bone Mineral Density in Premenopausal Women," Yeungnam University J. of Med., Vol. 24, No. 2S, pp. 330-339, 2007.
  4. Johnston, C. C. Jr., Hui, S. L., Witt, R. M., Appledorn, R., Baker, R. S. and Longcope, C., "Early menopausal changes in bone mass and sex steroids," J. Clin. Endocrinol. Metab., Vol. 61, No. 5, pp. 905-911, 1985. https://doi.org/10.1210/jcem-61-5-905
  5. Han, X., Xu, Y., Wang, J., Pei, X., Yang, R., Li, N. and Li, Y., "Effect of cod bone gelatin on bone metabolism and bone microarchitecture in ovariectomized rats," Bone, Vol. 44, No. 5, pp. 942-947, 2009. https://doi.org/10.1016/j.bone.2008.12.005
  6. Bain, S. D., Jerome, C., Shen, V., Dupin-Roger, I. and Ammann, P., "Strontium ranelate improves bone strength in ovariectomized by positively influencing bone resistance determinants," Osteoporos. Int., Vol. 20, No. 8, pp. 1417-1428, 2008.
  7. Kim, H. C., Kang, J. D., Kyoung, C. W. and Kim, H. C., "Case-control study of risk factors of fracture due to osteoporosis," The Journal of the Korean Society of Fractures, Vol. 15, No. 1, pp. 7-14, 2002. https://doi.org/10.12671/jksf.2002.15.1.7
  8. Sehmisch, S., Galal, R., Kolios, L., Tezval, M., Dullin, D., Zimmer, S., Sruermer, K. M. and Stuermer, E. K., "Effect of low-magnitude, high-frequency mechanical stimulation in the rat osteopenia model," Osteoporos. Int., Vol. 20, No. 12, pp. 1999-2008, 2009. https://doi.org/10.1007/s00198-009-0892-3
  9. Pluijm, S. M., Tromp, A. M., Smit, J. H., Deeg, D. J. And Lips, P., "Consequences of vertebral deformities in older men and women," J. Bone Miner. Res., Vol. 15, No. 8, pp. 1564-1572, 2000. https://doi.org/10.1359/jbmr.2000.15.8.1564
  10. Kinney, J. H., Haupt, D. L., Balooch, M., Ladd, A. J. C., Ryaby, J. T. and Lane, N. E., "Three-Dimensional Morphometry of the L6 Vertebra in the Ovariectomized Rat Model of Osteoporosis: Biomechanical Implications," J Bone Miner. Res., Vol. 15, No. 10, pp. 1981-1991, 2000. https://doi.org/10.1359/jbmr.2000.15.10.1981
  11. Shen, V., Dempster, D. W., Birchman, R., Xu, R. and Lindsay, R., "Loss of cancellous bone mass and connectivity in ovariectomized rats can be restored by combined treatment with parathyroid hormone and estradiol," J Clin. Invest., Vol. 91, No. 6, pp. 2479-2487, 1993. https://doi.org/10.1172/JCI116483
  12. Ito, M., Nishida, A., Koga, A., Ikeda, S., Shiraishi, A., Uetani, M., Hayashi, K. and Nakamura, T., "Contribution of Trabecular and Cortical Components to the Mechanical Properties of Bone and Their Regulating Parameters," Bone, Vol. 31, No. 3, pp. 351-358, 2002. https://doi.org/10.1016/S8756-3282(02)00830-X
  13. Kaczmarczyk-Sedlak, I., Pytlik, M., Sliwiniski, L., Nowinska, B. and Juszczyk, J., "Effect of administration of retinol and etidronate on bone histomorphometric parameters in ovariectomized rats," Acta Poloniae Pharmaceutica, Vol. 61, No. 5, pp. 383-391, 2004.
  14. Jun, I., Tsuyoshi, T. and Shoichi, I., "Combined Treatment with Vitamin K2 and Bisphosphonate in postmenopausal Women with Osteoporosis," Yonsei Medical Journal, Vol. 44, No. 5, pp. 751-756, 2003. https://doi.org/10.3349/ymj.2003.44.5.751
  15. Rhee, Y., Hur, J. H., Won, Y. Y., Lim, S. K., Chi, W. Q., Kim, K. G. an d Kim Y. E., "Assessment of bone guality using finite element analysis based upon Micro-CT image," Clinics in Orthopedic Surgery, Vol. 1, No. 1, pp. 40-47, 2009. https://doi.org/10.4055/cios.2009.1.1.40
  16. Rho, J. Y., Hobatho, M. C. and Ashman, R. B., "Relations of Mechanical Properties to Density and CT Numbers in Human Bone," Medical Engineering and Physics, Vol. 17, No. 5, pp. 347-355, 1995. https://doi.org/10.1016/1350-4533(95)97314-F
  17. Kim, S. J., Kim, K. W. and Lee, J. H., "A study on the trabecular change of Femur according to 17 $\beta$-Estradiol Dosage in Ovariectomized Rat," The Journal of Korean Association of Maxillofacial Plastic and Reconstructive Surgeons, Vol. 22, No. 2, pp. 155-153, 2000.
  18. Kim, K. T., Cho, S. H., Lee, J. A., Cho, S. H. and Chung, J. K., "The Effect of Oophorectomy and Hysterectomy on Rat Spine and Tibia," Korean Society of Obstetrics and Gynecology, Vol. 36, No. 11, pp. 3784-3791, 1993.
  19. Woo, D. G., Lee, B. Y., Lim, D. H. and Kim, H. S., "Relationship between nutrition factors and osteopenia : Effects of experimental diets on immature bone," Journal of Mechanics, Vol. 42, No. 8, pp. 1102-1107, 2009.
  20. Woo, D. G., Kim, H. S., Ko, C. Y., Lee, B. Y., Tack, G. R. and Kim, Y. H., "Detecting and Tracking Changed of Morphologies and Mechanical Characteristics on the Lumbar Vertebrae of OVX rats using RP Technique and Micro-FE method," Key Engineering Materials, Vol. 321-323, pp. 1078-1081, 2006. https://doi.org/10.4028/www.scientific.net/KEM.321-323.1078
  21. Ko, C. Y., Woo, D. G., Kim, H. S. and Lee, B. Y., "Micro-CT Evaluation in Osteoporosis Model," Korean J. Phys. Anthropol., Vol. 18, No. 4, pp. 283-290, 2005. https://doi.org/10.11637/kjpa.2005.18.4.283
  22. Ko, C. Y., Woo, D. G., Lee, T. W., Kim, H. S., Lee, B. Y. and Tack, G. R., "Detecting and Tracking Changes of Mechanical Characteristics of the Trabecular and Cortical Bone in the Lumbar Vertebrae of an OVX Rat-Use of In-Vivo Micro-CT and Micro-FE Analysis," Journal of the Korean Society for Precision Engineering Vol. 24, No. 1, pp. 110-117, 2007.
  23. Brouwers, J. E., Van Rietbergen, B. and Bouxsein, M. L., "Influence of early and late Zoledronic Acid Administration on Vertebral Structure and Strength in Ovariectomized Rats," Calcif. Tissue Int., Vol. 83, No. 3, pp.186-191, 2008. https://doi.org/10.1007/s00223-008-9160-3
  24. Ko, C. Y., Lee, T. W., Woo, D. G., Kim, H. S., Lim, D. H., Kim, H. S. and Lee, B. Y., "Effect of Whole Body Vibration on Trabecular Bone in OVX Rats," Korean J. Phys. Anthropol., Vol. 20, No. 4, pp. 301-309, 2007. https://doi.org/10.11637/kjpa.2007.20.4.301
  25. Elliott, D. M. and Saver, J. J., "Young investigator award winner : Validation of the mouse and rat disc as mechanical models of the human lumbar disc," Spine, Vol. 29, No. 7, pp. 713-722, 2004. https://doi.org/10.1097/01.BRS.0000116982.19331.EA
  26. Gerasimenko, Y., Roy, R. R. and Edgerton, V. R., "Epidural Stimulation : Comparison of the spinal circuits that generate and control locomotion in rats, cats and humans," Exp. Neurol., Vol. 209, No. 2, pp. 417-425, 2008. https://doi.org/10.1016/j.expneurol.2007.07.015
  27. Sun, K. and Liebschner, M. A., "Biomechanics of prophylactic vertebral reinforcement," Spine, Vol. 29, No. 13, pp. 1428-1435, 2004. https://doi.org/10.1097/01.BRS.0000128760.94173.90