Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제36권2호
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  • Pages.87-93
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  • 2010
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  • 2234-7550(pISSN)
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  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
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미니돼지에서 자가 피부유래 간엽성 줄기세포를 이용한 상악동저 거상술

Maxillary sinus floor elevation using autogenous skin-derived mesenchymal stem cells in miniature pigs

  • 변준호 (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원) ;
  • 강은주 (경상대학교 수의과대학 수의산과학) ;
  • 맹근호 (경상대학교 수의과대학 수의산과학) ;
  • 노규진 (경상대학교 수의과대학 수의산과학) ;
  • 강동호 (경상대학교 의학전문대학원 신경외과학교실) ;
  • 이종실 (경상대학교 의학전문대학원 병리학교실) ;
  • 박봉욱 (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원)
  • Byun, June-Ho (Department Oral and Maxillofacial Surgery, School of Medicine and Institute of Health Science, Gyeongsang National University) ;
  • Kang, Eun-Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Maeng, Geun-Ho (College of Veterinary Medicine, Gyeongsang National University) ;
  • Rho, Gyu-Jin (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kang, Dong-Ho (Departments of Neurosurgery School of Medicine, Gyeongsang National University) ;
  • Lee, Jong-Sil (Departments of Pathology, School of Medicine, Gyeongsang National University) ;
  • Park, Bong-Wook (Department Oral and Maxillofacial Surgery, School of Medicine and Institute of Health Science, Gyeongsang National University)
  • 투고 : 2010.01.26
  • 심사 : 2010.03.18
  • 발행 : 2010.04.30
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초록

Introduction: In our previous studies, we isolated porcine skin-derived mesenchymal stem cells (pSDMSCs) from the ears of adult miniature pigs and evaluated the pluripotency of these pSDMSCs based on expressions of transcription factors, such as Oct-4, Sox-2, and Nanog. Moreover, the characteristic of mesenchymal stem cells was revealed by the expression of various mesenchymal stem cell markers, including CD29, CD44, CD90, and vimentin. The aim of this study was to evaluate in vivo osteogenesis after maxillary sinus lift procedures with autogenous pSDMSCs and scaffold. Materials and Methods: The autogenous pSDMSCs were isolated from the 4 miniature pigs, and cultured to 3rd passage with same methods of our previous studies. After cell membranes were labeled using a PKH26, $1{\times}10^{7}$ cells/$100{\mu}L$ of autogenous pSDMSCs were grafted into the maxillary sinus with a demineralized bone matrix (DBM) and fibrin glue scaffold. In the contralateral control side, only a scaffold was grafted, without SDMSCs. After two animals each were euthanized at 2 and 4 weeks after grafting, the in vivo osteogenesis was evaluated with histolomorphometric and osteocalcin immunohistochemical studies. Results: In vivo PKH26 expression was detected in all specimens at 2 and 4 weeks after grafting. Trabecular bone formation and osteocalcin expression were more pronounced around the grafted materials in the autogenous pSDMSCs-grafted group compared to the control group. Newly generated bone was observed growing from the periphery to the center of the grafted material. Conclusion: The results of the present study suggest that autogenous skin-derived mesenchymal stem cells grafting with a DBM and fibrin glue scaffold can be a predictable method in the maxillary sinus floor elevation technique for implant surgery.

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참고문헌

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