Effects of Combined Treatment with Ultrasound and Ascorbic Acid on the Storage Qualities of Fresh-cut 'Jonathan'Apples

초음파와 ascorbic acid의 병용처리가 신선절단 '홍옥' 사과의 저장 중 품질에 미치는 영향

  • Jang, Ji-Hyun (Department of Food Science & Technology, Kyungpook National University) ;
  • Moon, Kwang-Deog (Department of Food Science & Technology, Kyungpook National University)
  • Received : 2009.12.29
  • Accepted : 2010.04.02
  • Published : 2010.04.30

Abstract

The effects of ultrasound treatment, in combination with ascorbic acid, on the quality of fresh-cut 'Jonathan' apples was investigated. Prepared apple slices were ultrasonicated in distilled water (US) or in 1%(w/v) ascorbic acid solution (UA) and the other samples were just dipped in 1%(w/v) ascorbic acid solution (AA). All samples were stored at$10^{\circ}C$ for 12 days. UA-treated samples showed high $L^{*}$ and hue values and low $a^{*}$, $b^{*}$, chroma, and ${\Delta}E$ value. Both control and US-treated samples showed considerable browning. A significant inhibition of polyphenol oxidase activity was observed after UA treatment. The level of total phenolics in UA-treated samples was higher on the day of treatment compared with other samples. Total soluble solids, pH, titratable acidity, and gas concentrations were similar in all samples. This study demonstrated that the simultaneous treatment of ultrasound and ascorbic acid was effective in preventing enzymatic browning of fresh-cut 'Jonathan' apples and maintaining total phenolics contents.

신선절단 '홍옥' 사과의 저장 중 품질에 대한 초음파 및 ascorbic acid의 병용처리의 효과를 조사하였다. 준비한 사과 절편을 초음파 처리(US), ascorbic acid 용액 침지처리(AA), 초음파 및 ascorbic acid 병용 처리(UA) 하였으며 $10^{\circ}C$에서 12일간 저장하였다. UA 처리구에서는 높은 $L^{*}$ 및 hue 값과 낮은 $a^{*}$, $b^{*}$, chroma 및 ${\Delta}E$값이 관찰되었으나 반면 무처리구와 US 처리구에서는 상당한 수준의 갈변이 관찰되었다. UA 처리에 의하여 유의적으로 높은 수준의 polyphenol oxidase 활성 억제가 확인되었으며 처리 당일 UA 처리구에서 다른 처리구에 비해 높은 수준의 총 페놀성 화합물 함량이 측정되었고 모든 처리구에서 총 가용성 고형분 함량, pH, 적정산도 및 포장 내 가스 농도는 큰 차이를 보이지 않았다. 따라서 초음파와 ascorbic acid의 병용처리가 신선절단 '홍옥' 사과의 효소적 갈변억제와 총 페놀성 화합물 함량의 유지에 효과가 있음을 확인하였다.

Keywords

References

  1. Pollack, S.L. (2001) Consumer demand for fruit and vegetables: The U.S. Example. In: Changing structure of global food consumption and trade. Regrni, A.(Editor), DIANE Publishing, Washington, DC, p,49-54
  2. Rico, D., Martin-Diana, A.B., Barat, J.M. and BarryRyan, C. (2007) Extending and measuring the quality of fresh-cut fruit and vegetables: a review. Trends Food Sci. Technol., 18, 373-386 https://doi.org/10.1016/j.tifs.2007.03.011
  3. Son, S.M., Moon, K.D. and Lee, C.Y. (2001) Inhibitory effects of various antibrowning agents on apple slices. Food Chem., 73, 23-30 https://doi.org/10.1016/S0308-8146(00)00274-0
  4. Ohlsson, T. (1994) Minimal processing-preservation methods of the future: an overview. Trends Food Sci. Technol., 5, 341-344 https://doi.org/10.1016/0924-2244(94)90210-0
  5. Soliva-Fortuny, R.C. and Martin-Belloso, O. (2003) New advances in extending the shelf-life of fresh-cut fruits: a review. Trends Food Sci. Technol., 14, 341-353 https://doi.org/10.1016/S0924-2244(03)00054-2
  6. Sapers, G.M., Hicks, K.B. and Miller, R.L. (2002) Antibrowning agents. In: Food additives. Branen, A.L., Davidson, P.M., Salminen, S., Thorngate, J.H. III.(Editors), Marcel Dekker Inc., New York, p.543 - 561
  7. Gil, M.I., Gorny, J.R. and Kader, A.A. (1998) Responses of 'Fuji' apple slices to ascorbic acid treatments and low-oxygen atmospheres. Hortscience, 33, 305-209
  8. Luo, Y. and Barbosa-Canovas, G.V. (1997) Enzymatic browning and its inhibition in new apple cultivars slices using 4-hexylresorcinol in combination with ascorbic acid. Food Sci. Technol. Int., 3, 195-201 https://doi.org/10.1177/108201329700300307
  9. Arias, E., Gonzalez, J., Oria, R. and Lopez-Buesa, P. (2007) Ascorbic acid and 4-hexylresorcinol effects on pear PPO and PPO catalyzed browning reaction. J. Food Sci., 72, 422-429 https://doi.org/10.1111/j.1750-3841.2007.00484.x
  10. Montogomery, M.W., and Petropakis, H.J. (1980) Inactivation of Bartlett pear polyphenol oxidase with heat in the presence of ascorbic acid. J. Food Sci., 45, 1090-1091 https://doi.org/10.1111/j.1365-2621.1980.tb07529.x
  11. Altunkaya, A. and Gokmen, V. (2008) Effect of various inhibitors on enzymatic browning, antioxidant activity and total phenol content of fresh lettuce (Lactuca sativa). Food Chem., 107, 1173-1179 https://doi.org/10.1016/j.foodchem.2007.09.046
  12. Rojas-Grau, M.A., Sobrino-Lopez, A., Tapia, M.S. and Martin-Belloso, O. (2006) Browning inhibition in freshcut 'Fuji' apple slices by natural antibrowning agents. J. Food Sci., 71, 59-65
  13. Ozoglu, H. and Bayindirli, A. (2002) Inhibition of enzymic browning in cloudy apple juice with selected antibrowning agents. Food Control, 13, 213-221 https://doi.org/10.1016/S0956-7135(02)00011-7
  14. Mason, T.J. and Paniwnyk, L. (2003) Ultrasound as a preservation technology. In: Food preservation techniques. Zeuthen, P.; Bogh-Sorensen, L.(Editors), CRC Press, New York, p.303-337
  15. Morris, C., Brody, A.L. and Wicker, L. (2007) Non-thermal food processing/preservation technologies: a review with packaging implications. Packag. Technol. Sci., 20, 275-286 https://doi.org/10.1002/pts.789
  16. Saftner, R.A., Abbott, J., Bhagwat, A.A. and Vinyard, B. (2005) Quality measurement of intact and fresh-cut slices of Fuji, Granny Smith, Pink Lady, and GoldRush apples. J. Food Sci., 70, 317-324 https://doi.org/10.1111/j.1365-2621.2005.tb11474.x
  17. Jang, J.-H., Kim, S.-T. and Moon, K.-D. (2009) Inhibitory effects of ultrasound in combination with ascorbic acid on browning and polyphenol oxidase activity of fresh-cut apples. Food Sci. Biotechnol., 18, 1417-1422
  18. Rojas-Grau, M.A., Soliva-Fortuny, R. and MartinBelloso, O. (2008) Effect of natural antibrowning agents on color and related enzymes in fresh-cut Fuji apples as an alternative to the use of ascorbic acid. J. Food Sci., 73, 267-272 https://doi.org/10.1111/j.1750-3841.2008.00794.x
  19. Park, J.Y. (1988) Apple cultivation. In: Standard agriculture manual. Park, S.H.,(Editor) Rural development administration, Suwon, p.33-36
  20. Mdluli, K.M. (2005) Partial purification and characterisation of polyphenol oxidase and peroxidase from marula fruit (Sclerocarya birrea subsp. Caffra). Food Chem., 92, 311-323 https://doi.org/10.1016/j.foodchem.2004.07.026
  21. Weller, A., Sims, C.A., Matthews, R.F., Bates, R.P. and Brecht, J.K. (1997) Browning susceptibility and changes in composition during storage of carambola slices. J. Food Sci., 62, 256-260 https://doi.org/10.1111/j.1365-2621.1997.tb03980.x
  22. Folin, O. and Denis, W. (1912) On phosphotungstic-phosphomolybdic compounds as color reagents. J. BioI. Chem., 12, 239-243
  23. MonsaIve-GonzaIez, A., Barbosa-Canovas, G.V., Cavalieri, R.P., McEvily, A.J. and Iyengar, R. (1993) Control of browning during storage of apple slices preserved by combined methods. 4-hexylresorcinol as anti-browning agent. J. Food Sci., 58, 797-800 https://doi.org/10.1111/j.1365-2621.1993.tb09361.x
  24. Sapers, G.M. and Douglas, F.W. (1987) Measurement of enzymatic browning at cut surfaces and in juice of raw apple and pear fruits. J. Food Sci., 52, 1258-1285. https://doi.org/10.1111/j.1365-2621.1987.tb14057.x
  25. Golan-Goldhirsh, A. and Whitaker, J.R. (1984) Effect of ascorbic acid, sodium bisulfite, and thiol compounds on mushroom polyphenol oxidase. J. Agric. Food Chem., 32, 1003-1009 https://doi.org/10.1021/jf00125a013
  26. Murata, M., Kurokami, C., Homma, S. and Matsuhashi, C. (1993) Immunochernical and immunohistochemical study of apple chlorogenic acid oxidase. J. Agric. Food Chem., 41, 1385-1390 https://doi.org/10.1021/jf00033a008
  27. de Almeida Teixeira, G.H., Durigan, J.F., Mattiuz, B.-H., Alves, R.E. and O'Hare, T.J. (2006) Cultivar affects browning susceptibility of freshly cut star fruit slices. Scientia Agricola., 63, 1-4
  28. Walter, W.M. and Purcell, A.E. (1980) Effect of substrate levels and polyphenol oxidase activity on darkening in sweet potato cultivars. J. Agric. Food Chem., 28, 941-944 https://doi.org/10.1021/jf60231a031
  29. Lee, S.A., Park, H.W., Kim, S.H., Park, J.D. and Kim, Y.H. (2007) Hot water treatment and modified atmosphere packaging affect the freshness extension of 'Fuji' apples. Korean J. Food Preserv., 14, 42-26
  30. Hwang, T.Y., Son, S.M., Lee, C.Y. and Moon, K.D. (2001) Quality changes of fresh-cut packaged Fuji apples during storage. Korean J. Food Sci. Technol., 33, 469-473