Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Changes in Antioxidant Enzyme Activities in the Gill And Digestive Glands of the Manila Clam Ruditapes philippinarum exposed to Cu

  • Min, Eun-Young (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Jang, Suck-Woo (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Sung-Gil (National Fisheries Research and Development Institute) ;
  • Kang, Ju-Cban (Department of Aquatic Life Medicine, Pukyong National University)
  • Received : 2008.12.04
  • Accepted : 2010.03.12
  • Published : 2010.03.31
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Abstract

We investigated the effect of Cu exposure on the activities of protective antioxidant enzymes in the gills and digestive glands of the manila clam Ruditapes phillippinarum exposed to subchronic concentrations (0, 20, 40, and $80{\mu}gL^{-1}$) of waterborne Cu. No mortality occurred during the experimental period, and no significant condition index differences were observed in any exposure group compared with the control. No significant differences were observed in the digestive glands and gills of the clams observed during 15 days of exposure, but after 30 days, the SOD activity in the gill showed a significant difference between the $80{\mu}gL^{-1}$ Cu-exposed group and the control. GPx activities in the digestive glands and gills were significantly lower after 30 days of Cu exposure. Gill GR activity in the high-exposure group ($80{\mu}gL^{-1}$) was significantly elevated compared with that in the control group. GST activities in the digestive glands of all groups did not change over 30 days. However, GST activity in the gill at $80{\mu}gL^{-1}$ Cu was significantly higher after 15 and 30 days of exposure. GSH activities in the gill showed patterns similar to those of GST activities during exposure periods. In the digestive glands, GSH activity was higher only at $80{\mu}gL^{-1}$ after 30 days exposure. In digestive glands and gills, the MDA levels of clams exposed to $80{\mu}gL^{-1}$ Cu were significantly higher after 30 days of exposure.

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References

  1. Bayne BL, Clarke KR and Gray JS. 1988. Background and rationale to a practical workshop on biological effects of pollutants. Mar Eco Prog Ser 46, 1-5. https://doi.org/10.3354/meps046001
  2. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochem 72, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  3. Buetler TM and Eaton DL. 1992. Glutathione S-transferases-amino-acid-sequence comparison, classification and phylogeneticre lationship. J Environ Sci Health 10C, 181-203.
  4. Carlberg I and Mannervik B. 1985. Glutathione reductase. Met Enzymol 113, 485-490.
  5. Cossu C, Doyotte A, Jacquin MC, Babut M, Exinger A and Vasseur P. 1997. Glutathione Reductase, Selenium-Dependent Glutathione Peroxidase, Glutathione Levels and Lipid Peroxidation in Freshwater Bivalves, Unio tumidus, as Biomarkers of Aquatic Contamination in Field studies. Ecotoxic Environ Safe 38, 122-131. https://doi.org/10.1006/eesa.1997.1582
  6. Dandapat J, Raok KJ and Chainy GBN. 1999. An in vitro study of metal ion-induced lipid peroxidation in giant fresh water prawn, Macrobrachium rasenbergii. Biometals 12, 89-97. https://doi.org/10.1023/A:1009227732623
  7. DeLeve LD and Kaplowitz N. 1990. Importance and regulation of hepatic glutathione. Seminars in Liver Disease 10, 251-266. https://doi.org/10.1055/s-2008-1040481
  8. Di Giulio PCW, Wenning RJ, Winston GW and Jewell CS. 1989. Biochemical response in aquatic animals; A review of determinants of oxidative stress. Environ Toxicol Chemical 8, 1103-1123. https://doi.org/10.1002/etc.5620081203
  9. Di Giulio PCW, Habig C and Gallagher FP. 1993. Effects of Black Rock Harbor sediments on indices of biotransformation, oxidative stress and DNA integrity in channel catfish. Aqua Toxicol 26, 1-22. https://doi.org/10.1016/0166-445X(93)90002-I
  10. Di Giulio JVB, Carlson DB, Hasspieler BM and Watson DF. 1995. Determinants of species susceptibility to oxidative stress: A comparison of channel catfish and brown bullhead. Mar Environ Res 39, 175-179. https://doi.org/10.1016/0141-1136(94)00021-G
  11. Doyotte A, Cossu C, Jacquin MC, Babut M and Vasseur P. 1997. Antioxidant enzymes, glutathione and lipid peroxidation of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve, Unio tumidus. Aqua Toxicol 39, 93-110. https://doi.org/10.1016/S0166-445X(97)00024-6
  12. Duquesne S, Liess M and Bird DJ. 2004. Sub-lethal effects of metal exposure: physiological and behavioural responses of the estuarine bivalve, Macoma balthica. Mar Environ Res 58, 245-250. https://doi.org/10.1016/j.marenvres.2004.03.066
  13. Flohe L and Otting F. 1984. Superoxide dismutase assays. Met Enzymol 105, 93-104. https://doi.org/10.1016/S0076-6879(84)05013-8
  14. Fridovich I. 1989. Superoxide dismutases. An adaptation to a paramagnetic gas. J Bio Chem 264, 7761-7764.
  15. Geret F, Serafim A, Barreira L and Bebianno M. 2002. Response of antioxidant systems to copper in the gill of the clam, Ruditapes decussantus. Mar Environ Res 54, 143-417.
  16. Geret F, Serafim A and Bebianno MJ. 2003. Antioxidant enzyme activities, metallothioneins and lipid peroxidation as biomarkers in Ruditapes decussantus. Ecotoxicology 12, 417-426. https://doi.org/10.1023/A:1026108306755
  17. Habig WH and Jacoby WE. 1981. Assays for differentiation of glutathione S- Transferases. Met Enzymol 77, 398-405. https://doi.org/10.1016/S0076-6879(81)77053-8
  18. Hasspieler BM, Behar JV, Carlson DB, Watson DE and Di Giulio RT. 1994a. Susceptibility of channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurus nebulolosus) to oxidative stress: A comparative study. Aqua Toxicol 28, 53-64. https://doi.org/10.1016/0166-445X(94)90020-5
  19. Hasspieler BM, Behar JV, Carlson DB, Watson DE and Di Giulio RT. 1994b. Glutathione-dependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurus nebulosus). Ecotoxicol Environ Safe 28, 82-90. https://doi.org/10.1006/eesa.1994.1036
  20. John AB and Steven DA. 1978. Microsomal lipid peroxidation. Met Enzymol 52, 302-310. https://doi.org/10.1016/S0076-6879(78)52032-6
  21. Livingstone DR. 1993. Biotechnology and pollution monitoring. Use of molecular biomarkers in the aquatic environment. J Chem Tech Biotech 57, 195-211.
  22. Livingstone DR, Lemaire P, Matthews A, Peters LD, Porte C, Fitzpatrick PJ, Forlin I, Nasci C, Fossato V, Wootton N and Goldfarb P. 1995. Assessment of the impact of organic pollutants on goby (Zostericessor ophiocephalus) and mussel (Mytilus galloprovincialis) from the Venice Lagoon, Italy: Biochemical studies. Mar Environ Res 39, 235-240. https://doi.org/10.1016/0141-1136(94)00055-T
  23. Mannervik B and Danielson UH. 1988. Glutathione transferase structure and catalytic activity. CRC Critical Review in Biochemistry 23, 283-337. https://doi.org/10.3109/10409238809088226
  24. Meister A and Anderson ME. 1983. Glutathione. Annual Review Biochemistry 52, 711-760. https://doi.org/10.1146/annurev.bi.52.070183.003431
  25. Meister A. 1984. New developments in glutathione metabolism and their potential application in therapy. Hepatology, 4, 739-742. https://doi.org/10.1002/hep.1840040431
  26. Paglia DE and Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clinic Med 70, 158-169.
  27. Power A and Sheehan D. 1996. Seasonal variation in the antioxidant defence of gill and digestive gland of the blue mussel, Mytilus edulis. Com Biochem Physi 114C, 99-103.
  28. Prohaska JR. 1980. The glutathione peroxidase activity of glutathione S-transferase. Biochemica et Biophysica Acta 611, 87-98. https://doi.org/10.1016/0005-2744(80)90045-5
  29. Regoli F and Orlando E. 1994. Accumulation and subcellular distribution of metals (Cu, Fe, Mn, Pb and Zn) in the mediterranean mussel, Mytilus galloprovincialis during a field transplant experiment. Mar Pollu Bull 28, 592-600. https://doi.org/10.1016/0025-326X(94)90360-3
  30. Regoli F and Principato G. 1995. Glutathione, glutathione-dependent and antioxidant enzymes in mussel, Mytilus gallopronvincialis, exposed to metals under field and laboratory conditions; Implications for the use of biochemical biomarkers. Aqua Toxicol 31, 143-164. https://doi.org/10.1016/0166-445X(94)00064-W
  31. Ribera D, Narbonne JF, Daubeze M and Michel X. 1989. Characterisation tisssue distribution and sexual differences of some paramaeters related to lipid peroxidation in mussels. Mar Environ Res 28, 279-283. https://doi.org/10.1016/0141-1136(89)90244-4
  32. Richardson RJ and Murphy SD. 1975. Effect glutathione on tissue deposition on methylmercury in rat. Toxicol Applied Pharma 31, 505-519. https://doi.org/10.1016/0041-008X(75)90274-4
  33. Rodriguez-Ariza A, Peinado J, Pueyo C and Lopez-Barea J. 1993. Biochemical indicators of oxidative stress in fish from polluted littoral areas. Can J Fish Aqua Sci 50, 2568-2573. https://doi.org/10.1139/f93-280
  34. Romeo M and Gnassia-Barelli M. 1997. Effect of heavy metals on lipid peroxidation in the Mediterranean clam, Ruditapes decussatus. Com Bioche Physio 118C, 33-37.
  35. Sheehan D and Power A. 1996. Seasonal variation in the antioxidant defence systems of gill and digestive gland of the blue mussel, Mytilus edulis. Com Biochem Physiol 114C, 99-103.
  36. Sheehan D and Power A. 1999. Effects of seasonality on xenobiotic and antioxidant defence mechanisms of bivalve molluscs. Com Biochem Physio 123C, 193-199.
  37. Sole M, Porte C and Albaiges J. 1995. Seasonal variation in the mixed function oxidase system and antioxidant enzymes of the mussel Mutilus galloprovincialis. Environ Toxicol Chem 14, 157-164. https://doi.org/10.1002/etc.5620140118
  38. Stegeman JJ, Brouver M, Di Giulio RT, Forlin L, Fowler BA, Sanders BM and Van Veld PA. 1992. Molecular responses to environmental contamination: Enzyme and protein systems as indicators of chemical exposure and effect. In Biomarker, Biochemical, Physiological, and Histological Markers of Anthropogenic Stress (R.I. Huggett, R. A. Kimerle, P. M. Mehrle and Bergman, Eds.), pp. 235-335. Lewis.
  39. Suteau P, Daubeze M, Migaud ML and Narbonne JF. 1988. PAH-metabolizing enzymes in whole mussel as biochemical tests for chemical pollution monitoring. Mar Eco Progress Series 46, 45-49. https://doi.org/10.3354/meps046045
  40. Viarengo A, Canesi I, Pertica M, Livingstone DR and Orunesu M. 1991a. Age-related lipid peroxidation in the digestive gland of mussels. The role of the antioxidant defense systems. Experientia 47, 454-457. https://doi.org/10.1007/BF01959942
  41. Viarengo A, Canesi I, Pertica M and Livingstone DR. 1991b. Seasonal variations in the antioxidant defense systems and lipid peroxidation of the digestive gland of mussels. Com Biochem Physio 100C, 187-190.
  42. Wang W and Ballatori N. 1998. Endogenous glutathione conjugates: occurrence and biological functions. Pharmacological Reviews, 50, 335-356.
  43. Winston GW and Di Giudio RT. 1991. Prooxidant and antioxidant mechanisms in aquatic organisms. Aqua Toxicol 19, 137-161. https://doi.org/10.1016/0166-445X(91)90033-6