Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지

Microbiological Safety Assessment to Secure Safety of Food Service in University

대학 내 급식소의 안전성 확보를 위한 미생물학적 안전성 평가

  • Kim, Kyeong-Yeol (Division of Applied Life Science (BK 21 program), Graduate School, Gyeongsang National University) ;
  • Nam, Min-Ji (Division of Applied Life Science (BK 21 program), Graduate School, Gyeongsang National University) ;
  • Nam, Bo-Ram (Division of Applied Life Science (BK 21 program), Graduate School, Gyeongsang National University) ;
  • Ryu, Hee-Jung (Division of Applied Life Science (BK 21 program), Graduate School, Gyeongsang National University) ;
  • Heo, Rok-Won (Division of Applied Life Science (BK 21 program), Graduate School, Gyeongsang National University) ;
  • Shim, Won-Bo (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Chung, Duck-Hwa (Division of Applied Life Science (BK 21 program), Graduate School, Gyeongsang National University)
  • 김경열 (경상대학교 응용생명과학부(BK 21 program)) ;
  • 남민지 (경상대학교 응용생명과학부(BK 21 program)) ;
  • 남보람 (경상대학교 응용생명과학부(BK 21 program)) ;
  • 류희정 (경상대학교 응용생명과학부(BK 21 program)) ;
  • 허록원 (경상대학교 응용생명과학부(BK 21 program)) ;
  • 심원보 (경상대학교 농업생명과학연구원) ;
  • 정덕화 (경상대학교 응용생명과학부(BK 21 program))
  • Received : 2009.12.22
  • Accepted : 2010.01.19
  • Published : 2010.03.31
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Abstract

The objectives of this study were to investigate the microbial contamination levels on food service in university and to provide the information of microbial contamination to improve food safety. A total of 288 samples were collected during summer and winter season between 2006 and 2008 from 4 food services located in the university in Western Gyeongnam and were used to detect sanitary indicator bacteria [aerobic plate count (APC), coliform, and Escherichia coli] and pathogenic bacteria (Staphylococcus aureus, Salmonella spp.). As a result, APC and coliform for hand and kitchen utensils which are used often by the employee were detected at high levels of 1.1~5.5 and 1.3~5.3 log CFU/($100\;cm^2$, hand), respectively. The contamination levels of APC and coliform in cooked foods and drinking water were 0.8~6.4 and 1.3~5.0 log CFU/(g, mL), respectively. Especially, the cooked foods showed the highest contamination for APC (2.1~6.4 log CFU/g) and coliform (1.0~5.0 log CFU/g). We think the reason that the cooked foods may be contaminated with APC and coliform on cooking process by using employee's hand and kitchen utensils. Moreover, S. aureus for hand and kitchen utensils was detected at levels of 2.8~3.0 and 2.0~2.3 log CFU/(g, hand), but Salmonella spp. was not detected. According to the above results, contamination levels of the samples were mostly decreased irrespective of summer and winter season. The results obtained indicated that it is necessary to periodic monitoring for microorganism contamination and education about personal and environmental hygiene to employee for ensuring food safety of food service in university.

본 연구는 서부경남지역에 소재하는 대학 내 급식소에 대하여 미생물학적 안전성 평가를 실시하고, 안전성 확보를 위한 미생물학적 정보를 제공하기 위해 수행되었다. 본 연구를 위하여 서부경남지역에 소재하는 대학 내 급식소 4곳을 대상으로 2006년부터 2008년까지 하절기와 동절기로 구분하여 개인위생, 음용수, 조리된 음식 및 조리도구와 관련된 항목들에 대하여 위생지표세균과 병원성 미생물에 대한 미생물학적 위해 분석을 실시하였다. 원료와의 접촉 빈도가 높은 칼, 도마 및 행주 등의 조리도구와 조리종사자의 손에 대하여 일반세균과 대장균군이 각각 1.1~5.5와 1.3~5.3 log CFU/($100\;cm^2$, hand)으로 높게 검출되었고, 조리된 음식과 음용수에서도 일반세균과 대장균군이 각각 0.8~6.4와 1.3~5.0 log CFU/(g, mL)으로 비교적 높은 오염도를 나타내어 조리과정 중의 교차오염의 발생이 우려되었다. 또한 병원성 미생물 중 S. aureus가 조리종사자의 손과 조리된 음식에서 각각 2.8~3.0과 2.0~2.3 log CFU/(hand, g)로 비교적 높은 수준으로 검출되어 조리과정 중 개인위생불량으로 인한 교차오염 발생 가능성이 높은 것으로 확인되었고, E. coli와 Salmonella spp.는 모든 시료에서 검출되지 않았다. 따라서 대학 내 급식소의 안전성을 확보하기 위해서는 농산물우수관리제도(GAP) 등에 의해 생산된 안전한 원료를 위해요소중점관리제도(HACCP system) 매뉴얼에 적용하여 운영될 수 있도록 널리 보급하고, 조리종사자에 대한 철저한 개인위생관리와 주기적인 위생교육을 실시함으로써 원료 입고에서 배식까지의 모든 과정에 대한 위해요소를 사전에 차단 할 수 있을 것이라고 판단된다.

Keywords

References

  1. Park, S.W. and Ha, K.H.: Assessment of university food service by students in Daejeon Area. Korean J. Food Nutr. 11(5), 528-535 (1998).
  2. Park, S.W., Chang, Y.S.: A survey on the utilization university food service by student in Daejeon city. Korean J. Soc. Food Sci.14(4), 400-406 (1998).
  3. Han, M.J.: A survey of college students opinions on school cafeteria in Seoul area. Korean Soc. Food Culture. 7(2), 113- 118 (1992).
  4. Roh, J.M., Yoo, Y.S.: A study on the state of eating out and food preference of college students in Seoul (2). Korean Home Ecomomics Assoc. 27(4), 51-58 (1989).
  5. Han, M.J., Yoon, J.Y., Kim, N.Y., Yoo, Y.H.: Satisfaction of meal and service quality in university foodservice institutions. Korean J. Soc. Fd Cookery Sci. 20(6), 545-552 (2004).
  6. Yang, I.S., Lee Y.E., Kim D.H.: Measuring attitude and satisfaction of yonsei university students towards contracted vs. rented university foodservice. Korean J. Dietary Culture. 13(5), 423 (1998).
  7. Kim, S.H., Lee, Y.W.: A study on the sanitary management procedures of university and industry foodservice operations in Pusan. J. Fd Hyg. Safety. 16(1), 1-10 (2001).
  8. Korea Food & Drug Administration. Available: http://www. foodnara.go.kr/service. Accessed June 12, (2009).
  9. Kwon, S.H., Lee, H.O., Chung, D.H., Shin W.S., Om, A.S.: The seasonal microbiological quality assessment for Application of HACCP system to the elementary school food service. Korean J. Soc. Food Cookery Sci. 19(5), 647-658 (2003).
  10. Eo, G.H., Ryu, k., Park, S.J., Kwak, T.K.: Need assessments of HACCP-based sanitation training program in elementary school foodservice operations based on sanitization knowledge test of employees. J. Korean Diet. Assoc. 7(1), 56-64 (2001).
  11. Kim J.Y., Kim, S.R., Choi, J.G., Je, J.H., Chung, D.H.: Assessment of the level of microbial contamination in the processing company of sandwich products. Korean J. Env. Heal. 32(4), 316-323 (2006).
  12. Kim, K.Y., Nam M.J., Lee, H.W., Shim, W.B., Yoon, Y.H., Kim, S.R., Kim, D.H., Ryu, J.G., Hong, M. K., You, O.J., Chung, D.H.: Microbiological safety assessment of a perilla leaf postharvest facility for application of a good agricultural practices (GAP) system. Korean J. Food Sci. Technol. 41(4), 392-398 (2009).
  13. Lee, H.W., Yoon, Y.H., Seo, E.K., Kim, K.Y., Shim, W.B., Kil, J.K., Chung, D.H.: Microbiological hazard analysis for agricultural products processing center of tomato and recom mendations to introduce good agricultural practices (GAP) system. Korean J. Food Sci. Technol. 41(2), 210-214 (2009).
  14. Synder, O.P.: HACCP-an industry food safety self-control program-part VII (Basic considerations in environment control). Dairy Food and Environ. Sanitat.12, 574-577 (1992).
  15. Park, H.K., Kim, K.L., Shin, H.W., Kye, S.H., Yoo, W.C.: Evaluation of microbiological hazards of cooking utensils and environment of mass catering establishments. J. Fd Hyg. Safety. 15(4), 315-323 (2000).
  16. Beuchat, L.R.: Listeria monocytogenes - incidence on vegetables. Food Cont. 7, 222-228 (1996).
  17. Choi, J.W., Park, S.Y., Yeon, J.H., Lee, M.G., Chung, D.H., Lee, K.H., Kim, M.G., Lee, D.H., Kim, K. S., Ha, S.D.: Microbial Contamination Levels of Fresh Vegetables Distributed in Markets. J. Fd Hyg. Safety. 20(1), 43-47 (2005).
  18. Kim, S.R., Shim, W.B., Park, S.J., Ha, K.S., Yoon, H.S., Ha, S.D., Kim, K.S., Lee, K.H., Kim, M.G., Kim, K.Y., Lee, C.H., Chung, D.H.: Investigation of the level of microbial contamination in the environment for juice production. Korean J. Food Sci. Technol. 37(2), 287-293 (2005).
  19. Korea Food and Drug Administration. Food Code, KFDA, Seoul (2008).
  20. Kim, S.R., Park, S.J., Shim, W.B., Kim, H.K., Chung, D.H.: Detection of Staphylococcus aureus and screening Staphylococcal enterotoxin a, b, c genes in strains isolated from strawberry juice shops in Jinju. Korean J. Env. Heal. 31(1), 23-30 (2005).
  21. AOAC. Official Method of Analysis of AOAC Intl. 17th ed. Association of Official Analytical communities, Arlington, VA, USA (1989).
  22. Martin, M.C., Fueyo, J.M., Gonzalez-Hevia, M.A., Mendoza, M.C.: Genetic procedures for identification of enterotoxingenic stains of Staphylococcus aureus from three food poisoning outbreak. Int. J. Food Microbiology. 94, 279-286 (2004). https://doi.org/10.1016/j.ijfoodmicro.2004.01.011
  23. Food and Drug Administration. Guidance for industry, Guide to minimize microbial food safety hazard for fresh fruits and vegetables. Available from: http://csan.fda.gov. Accessed September 26 (2009).
  24. Center for disease control: Outbreaks of Salmonella infections associated with eating Roma tomatoes-United states and Canada, 2004. Available from: http://www.cdc.gov. Accessed August 11 (2009).
  25. Ministry of Environment Republic of Korea: Drinking water quality standard and test. Available from : http://eng.me.go.kr. Accessed October 1 (2009).
  26. Silverman, G. T., Carpenter, D. F., Munsey, D. T., Rowley, D. B. J. : Microbiological evaluation of production procedures for frozen foil pack meals of the central preparation facility of the frances E. warren air force base. Technical Report 76- 37-FSL, U.S.Arm Natick Research and Development command, Natick, Mass (1976).
  27. Kwon, S.H., Lee, H.O., Chung, D.H., Shin W.S., Om, A.S.: The seasonal microbiolgical quality assessment for application of HACCP system to the elementary school food service. Korean J. Soc. Food Cookery Sci. 19(5), 647-658 (2003).
  28. Park, S.J., Ha, G.S., Shim, W.B., Park, M.K., Chung, D.H.: Environmental microbial assessment of food services at elementary schools in western Gyeongnam province. J. Fd Hyg. Safety. 18(1), 14-24 (2003).
  29. Bryan, F.L.: Factors that contribute to outbreaks of foodborne disease. J. Food Prot. 41, 816 (1978).
  30. Harrigan, W.F., McCance, M.E.: Laboratory methods in food and dairy microbiology. Academic Press Inc. Ltd., N. Y., USA (1976).
  31. Solberg, M., Buckalew, J.J., Chen, C.M., Schaffner, D.W., O'Neill, K., McDowell, J., Post, L.S., Boderck, M.: Microbiological safety assurance system for foodservice facilities. Food Technol. 44(12), 68-73 (1990).
  32. Lee, H.S., Ryu, S.Y.: The seasonal microbiological quality assessment of kimbap(seaweed roll) production flow in foodservice facilities for univ. student - HACCP model-. Korean J. Soc. Food Sci. 14(4), 367-374 (1998).
  33. Hatakka, M., Bjorkoth, K.J., Asplund, K., Maki-Petays, N., Korkeala, H.J.: Genotypes and enterotoxicity of Staphylococcus aureus isolated from the hands and nasal cavities of flightcatering employees. J. Food Prot. 63, 1487-1491 (2000).
  34. Kim, J.S., Shim, W.B., Kim, J.H., Kim, S.R., Chung, D.H.: Sanitary microbial distribution at the tomato farms in western Gyeongnam. Korean J. Env. Heal. 32(1), 77-88 (2006).