Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Anti-Inflammatory Effect of Chlorella ellipsoidea Extracted from Seawater by Organic Solvents

해수산 클로렐라(Chlorella ellipsoidea) 유기용매 추출물의 항염증 효과

  • Choi, Yoo-Jin (Department of Parasitology, College of Medicine, Dong-A University) ;
  • Jo, Wol-Soon (Department of Parasitology, College of Medicine, Dong-A University) ;
  • Kim, Hyoun-Ji (Department of Parasitology, College of Medicine, Dong-A University) ;
  • Nam, Byung-Hyouk (Busan Techno-Park, Marine Bio-industry Development Center) ;
  • Kang, Eun-Young (Busan Techno-Park, Marine Bio-industry Development Center) ;
  • Oh, Su-Jung (Department of Parasitology, College of Medicine, Dong-A University) ;
  • Lee, Gye-An (NLP Co. LTD) ;
  • Jeong, Min-Ho (Department of Parasitology, College of Medicine, Dong-A University)
  • Received : 2009.12.02
  • Accepted : 2010.02.18
  • Published : 2010.02.28
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Abstract

Chlorella has been reported to have certain beneficial physiological effects, including hypocholesterolemic, antihypertensive, antioxidative, and anti-tumor activities in animal and human studies. The aim of this study was to determine the anti-inflammatory activities of an 80% methanol extract(CE-Met), hexane fraction (CE-Hex), and ethyl acetate fraction (CE-EA) of Chlorella ellipsoidea in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage. Treatment with various concentrations of the C. ellipsoidea extract resulted m a significant, dose-dependent reduction in nitric oxide (NO) production by LPS-induced macrophages. The C. ellipsoidea extract significantly inhibited LPS-induced NO production accompanied by an attenuation of IL-6 and TNF-$\alpha$ formation in macrophages. These results suggest potent inhibit이y effects on the production of inflammatory mediators by a C. ellipsoidea extract. Thus, C. ellipsoidea extract may be a potent anti-inflammatory agent for troubled skin.

Keywords

References

  1. Abad MJ, Bedoya LM and Bermejo P. 2008. Natural marine anti-inflammatory products. Mini-Reviews in Medicinal Chemistry 8, 740-754 https://doi.org/10.2174/138955708784912148
  2. Burja AM, Banaigs B, Abou-Mansour E, Burgess JG and Wright PC. 2001. Marine cyanobacteria-a prolific source of natural products. Tetrahedron 57, 9347- 9377. https://doi.org/10.1016/S0040-4020(01)00931-0
  3. Cabrera T, Bae JH, Bai SC and Hur SB. 2005. Comparison of the nutritional value of Chiarella ellipsoidea and Nannochloris oculata for rotifers and Artemianauplii. J Fish Sci Technol 8, 201-206. https://doi.org/10.5657/fas.2005.8.4.201
  4. Czarnecka OM, Bator WM and Silny W. 2005. Atopy patch test reaction to airborne allergens in the diagnosis ofatopi dermatitis. Acta Dennatovenerol Croat 13, 3-16.
  5. Fujiwara N and Kobayashi K. 2005. Macrophages in inflammation. Current Drug Targets Inflammation and Allergy 4, 271-286. https://doi.org/10.2174/1568010054022105
  6. Funk CD, Frunk LB, Kennedy ME, Pong AS and Fitzgerald A. 1991. Human platelet/erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression and gene chromosomal assignment. The Faseb Journal 5, 2304-2312. https://doi.org/10.1096/fasebj.5.9.1907252
  7. Guzman S, Gato A, Lamela M, Freire-Garabal M and Calleja JM. 2003. Anti-inflammatory and immunomodulatory activities of polysaccharide from Chlorella stigmatophora and Phaeodactylum tricornutum, Phytother Res 17, 665-670. https://doi.org/10.1002/ptr.1227
  8. Hatano S, Kabata K, Yoshimoto M and Sadakane H. 1982. Accumulation of free fatty acids during hardening of Chiarella ellipsoidea. Plant Physiol 70, 1173-1177. https://doi.org/10.1104/pp.70.4.1173
  9. Higuchi M, Hisgahi N, Taki H and Osawa T. 1990. Cytolytic mechanisms of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanisms act synergistically as the major cytolytic mechanisms of activated macrophages. The Journal of Immunology 144, 1425-1431.
  10. Hong YL, Kim MH, Ahn C, Lee HY and Kim JD. 2000. Studies on the biological activities of the extract from Hovenia dulcis Thunb. Inst Agr Sci 11, 1-11.
  11. Kay PA. 1991. Microalgae as food and supplement. Crit Rev Food Sci Nutr 30, 555-573. https://doi.org/10.1080/10408399109527556
  12. Kim SY, Park KJ and Lee WC. 1998. Antiinammaroty and antioxidative effects of Morus spp. Fruit extract. The Korean Society of Medical Crop Science 6, 204-209.
  13. Kundu JK and Surh YJ. 2005. Breaking the relay in deregulated cellular signal transduction asa rationale for chemoprevention with anti-inflammatory Phytochemicals. Mutation Research 591, 123-146. https://doi.org/10.1016/j.mrfmmm.2005.04.019
  14. Lee HS, Choi CY, Cho C and Song Y. 2003. Attenuating effect of Chlorella supplementation of oxidative stress and NF-kB activation in peritoneal macrophages and liver of C57BL/6 mice fed on an atherogenic diet. Biosci Biotechnol Biochem 67, 2083-2090. https://doi.org/10.1271/bbb.67.2083
  15. Lee KJ and Row KH. 2004. Comparision of extraction methods for aglycone isoflavones from Korean soybean. Korean J Biotechnol Bioeng 19, 421-426.
  16. Lee YH. 2007a. Effect of Phellinus Linteus grown in germinated brown rice on atopic dermatitis. The Korean Society of Cosmetology 13, 514-519.
  17. Lee YG and Cho JY. 2007b. Inhibitory effect of curcumin on nitric oxide production in lipopolysaccharidestimulated RAW 264.7 cells and its suppressive mechanism. Korean Journal of Medicinal Crop Science 15, 451-456.
  18. Marithasan S and Monack DM. 2007. Inflammasome adaptors and sensor: intracellular regulators of infection and inflammation. Nature Reviews Immunology 7, 31-40. https://doi.org/10.1038/nri1997
  19. Masferrer J, Zweifel BS, Manning PT, Hauser SD, Leahy KM, Smith WG, Isakson PC and Seibert K. 1994. Selective inhibition of inducible cyclooxygenase 2 in vivo is anti-inflammatory and nonulcerogenic. Proc Natl Acad Sci USA 91, 3228-3232. https://doi.org/10.1073/pnas.91.8.3228
  20. MeDaniel ML, Kwon G, Hill JR, Marshall CA and Corbett JA. 1996. Cytokines and nitric oxide in islet inflammation and diabetes. Proceeding of the Society for Experimental Biology and Medicine 211, 24-32. https://doi.org/10.3181/00379727-211-43950D
  21. Merchant RE, Andre CA and Sica DA. 2002. Nutritional supplementation with Chlorella pyrenoidosa for mildtomodcrate hypertension. J Med Food 5, 141-152. https://doi.org/10.1089/10966200260398170
  22. Morimoto T, Nagatsu A, Murakami N, Sakakibara J, Tokuda H, Nishino Hand Iwashima A. 1995. Anti-tumor promoting glyceroglycolipids from the green alga, Chlorella vulgaris. Phytochemistry 40, 1433-1437. https://doi.org/10.1016/0031-9422(95)00458-J
  23. Mukaida N, Ishikawa Y, Ikeda N, Fujioka N, Watanabe S and Kuno K. 1996. Novel insight into molecular mechanism of endoxotin shock: biochemical analysis of LPS receptor signaling in cell-free system targeting NF-kB and regulation of cytokine production/action through beta2 integrin in vivo. J Leukoc Biol 59, 145-151. https://doi.org/10.1002/jlb.59.2.145
  24. Mu MM, Chakravortty D, Sugiyama T, Koide N, Takahashi K, Mori I, Yoshida T and Yokochi T. 2001. The inhibitory action of quercetin on lipopolysaccharide-induced nitric oxide production in raw 264.7 macrophage cells. Journal of Endotoxin Research 7, 431-438. https://doi.org/10.1179/096805101101533034
  25. Park MK, Lee FM, Park CH and In MJ. 2002. Quality characteristics of sulgidduk containing Chlorella powder. J Korean Soc Food Sci Nutr 31, 225-229. https://doi.org/10.3746/jkfn.2002.31.2.225
  26. Paul A, Cuenda A, Bryant CE, Murray J, ChiIvers ER, Cohen P, Gould GW and Plevin R. 1999. Involvement of mitogen activated protein kinase homologues in the regulation of lipopolysaccharidemediated induction of cyclo-oxygenase-Z but not nitric oxide syntheses in RAW 264.7 macrophages. Cell Signalling 11, 491-497. https://doi.org/10.1016/S0898-6568(99)00018-2
  27. Scott MG and Hancock RE. 2000. Cationic antimicrobial peptides and their multifunctional role in the immune system. Crit Rev Immunol 20, 407-431.
  28. Seibert K, Zhang Y, Leahy K, Hauser S, Masferrer J, Perkins W, Lee L and Kaskson P. 1994. Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain. Proc Natl Acad Sci USA 91, 12013-12017. https://doi.org/10.1073/pnas.91.25.12013
  29. Shen T, Lee YJ and Cho JY. 2008. Effect of hot water extract from Scutellaria barbata on the macrophages activated by lipopolysaccharide. Korean Journal of Medicinal Crop Science 16, 313-319.
  30. Shibata S, ada K, Onodera-Masuoka N, Matsubara S, Kikuchi-Hayakawa H, Ishikawa F, Iwabuchi A and Sansawa H. 2001. Hypocholesterolemic effect of indigestible fraction of Chlorella valgaris in cholesterol-fed rat. J Nutr Sci Vitaminol 47, 373-377. https://doi.org/10.3177/jnsv.47.373
  31. Shim JY, Shin HS, Han JG, Park HS, Lim BL, Chung KW and am AS. 2008. Protective effects of Chlorella vulgaris on liver toxicity in cadmium administered rats. J Med Food 11, 479-485. https://doi.org/10.1089/jmf.2007.0075
  32. Stuehr HH, Kwon NS, Weise M and Nathan C. 1991. Purification of the cytokine-induced macrophage nitric oxide synthase: and FAD- and FMNcontaining flavoprotein. Proc Natl Acad Sci USA 88, 7773-7777. https://doi.org/10.1073/pnas.88.17.7773
  33. Willeaume V, Kruys V, Mijatovic T and Huez G. 1996. Tumor necrosis factor-alpha production induced by viruses and by lipopolysaccharides in macrophages: similarities and differences. Journal of Inflammation 46, 1-12.
  34. Yang JL, Jang JH, Radliakrishnan V, Kim YH and Song YS. 2008. $\beta$-Glucan suppresses LPSstimulated NO production through the downregulation of iNOS expression and NF-kB transactivation in raw 264.7 macrophages. Food Science and Biotechnology 17, 106-113.
  35. Yun JY, Choi SY, Park PJ, Chung HG, Shin HM, Suk K and Lim BO. 2008. Extract of Artemisia princeps Pampanini inhibit lipopolysaccarideinduced nitric oxide, cyclooxygenase2, prostaglandin E2, and tumor necrosis factor-a production from murine macrophages RAW 264.7 cells. Korean Journal of Medicinal Crop Science 16, 326-331.

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