Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Biodegradation of Endosulfan by Klebsiella oxytoca KE-8 Immobilized on Activated Carbon

  • Jo, Min-Sub (School of Bioresource Sciences, Andong National University) ;
  • Lee, Jung-Bok (Dept. of Food & Nutrition Science, Kundong University) ;
  • Kim, Jang-Eok (School of Biosciences, Kyungpook National University) ;
  • Sohn, Ho-Yong (Dept. of Food & Nutrition, Andong National University) ;
  • Jeon, Chun-Pyo (School of Bioresource Sciences, Andong National University) ;
  • Choi, Chung-Sig (HansBio Co. Research Center) ;
  • Kwon, Gi-Seok (School of Bioresource Sciences, Andong National University)
  • Received : 2010.03.22
  • Accepted : 2010.05.10
  • Published : 2010.06.30
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Abstract

Endosulfan degrading ability of Klebsiella oxytoca KE-8 immobilized by entrapment with activated carbon was examined. Endosulfan degradation by the immobilized bacterial strains on several different activated carbon based support materials was investigated. Based on results, activated carbon ($8\times30$ mesh) was chosen as a support material. The immobilized Klebsiella oxytoca KE-8 with the cell density of 4 mg $g^{-1}$ (dry weight) degraded 22.18 ug $ml^{-1}$ endosulfan within 5 days at pH 7.0, $30^{\circ}C$ in batch shake flask cultures. Also, we an experimented recycle packed bed column mode and continuous packed bed column mode for endosulfan degradation. Under optimum operation condition, the immobilized cells in a laboratory scale pack bed column with support beads were able to degrade endosulfan completely in defined minimal salt medium at a maximum rate of 129.6 ug $ml^{-1}$ per day. Moreover, the endosulfan degradation activity could be demonstrated at $4^{\circ}C$ for one month without significant decrease in activity. Results of this study suggest that immobilized cells of Klebsiella oxytoca KE-8 might be applicable to endosulfan contaminated site.

Keywords

References

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