Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Determination of 105 pesticide residues by GC/MS/MS

GC/MS/MS를 이용한 105종의 잔류농약 분석에 관한 연구

  • Kim, W.S. (Test & Analytical Laboratory, Busan Regional Food & Drug Administration) ;
  • Do, J.A. (National institute of Food and Drug Safety Evaluation, Korea Food & Drug Administration) ;
  • Lee, H.J. (Test & Analytical Laboratory, Busan Regional Food & Drug Administration) ;
  • Lee, J.Y. (Test & Analytical Laboratory, Busan Regional Food & Drug Administration) ;
  • Yang, S.J. (Agilent Korea) ;
  • Lee, S.H. (Medical Device Safety Bureau, Korea Food & Drug Administration)
  • 김우성 (부산지방식품의약품안전청 시험분석센터) ;
  • 도정아 (식품의약품안전평가원 화학물질과) ;
  • 이희정 (부산지방식품의약품안전청 시험분석센터) ;
  • 이주영 (부산지방식품의약품안전청 시험분석센터) ;
  • 양승주 (에질런트 코리아) ;
  • 이선희 (식품의약품안전청 의약품안전국)
  • Received : 2009.09.07
  • Accepted : 2010.06.10
  • Published : 2010.08.25
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Abstract

A multi-residual method using gas chromatography coupled with mass spectrometry (GC/MS/ MS) was developed for the analysis of 105 pesticides. This method was tested on lemons, beans and other vegetables. The pretreatment of these pesticides was performed by liquid-liquid partition followed by cleanup with solid phase extraction cartridge (SPE Florisil), after acetonitrile extraction from matrices and sodium chloride (15 g) addition. The recovery ranged from 71.1% to 126.0% except for azinphosmethyl, famoxadone, fenamidone, flufenoxuron and triadimefon in lemons and from 72.5% to 124.5% in bean. In lemon, the limit of detection (LOD) and limit of quantification (LOQ) were 0.001~150 ng/ mL and 0.004~500 ng/mL, respectively.

레몬과 콩 그리고 채소류에서 105개의 잔류농약을 다종 다성분 분석법으로 GC/MS/MS 분석하였다. 각 시료로부터 acetonitrile로 1차 추출하고 NaCl 15 g을 첨가한 다음 액-액 분배하였으며, Florisil 고체상(SPE) Cartridge로 정제하여 분석하였다. 레몬과 콩에서의 105개 농약의 회수율은 azinphos-methyl, famoxadone, fenamidone, flufenoxuron, triadimefon을 제외한 모든 농약에서 우수한 것으로 나타났는데 레몬에서 71.1~126.0%, 콩에서는 72.5~124.5% 였다. 또한 레몬에서의 검출한계와 정량한계는 각각 0.001~150 ng/mL와 0.004~500 ng/mL으로 확인되었다.

Keywords

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