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Effects of Continuously Cropped Soil Extracts on Cell Viability and Seedling Growth of Peony(Paeonia lactiflora)

작약 연작지토양 추출물이 작약 배양세포와 배양묘의 생육에 미치는 영향

  • Park, Jun-Hong (Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Choi, Seong-Yong (Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Park, So-Deuk (Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Kim, Tae-Hwa (School of Division Applied Biosciences, College of Agricultural Life Science, Kyungpook National University) ;
  • Park, Man (School of Division Applied Biosciences, College of Agricultural Life Science, Kyungpook National University) ;
  • Kim, Jang-Eok (School of Division Applied Biosciences, College of Agricultural Life Science, Kyungpook National University)
  • 박준홍 (경상북도농업기술원) ;
  • 최성용 (경상북도농업기술원) ;
  • 박소득 (경상북도농업기술원) ;
  • 김태화 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 박만 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 김장억 (경북대학교 농업생명과학대학 응용생명과학부)
  • Received : 2010.03.02
  • Accepted : 2010.03.23
  • Published : 2010.03.31

Abstract

This experiment was conducted to investigate the growth inhibition effects caused by continuous cropping soil in peony(Paeonia lactiflora Pallas). The effect of extracts from continuous cropping soil of peony was tested with bio-assay method using callus cells induced from peony filament tissues and seedlings derived from peony zygotic embryos. The cell viability and seedling growth were significantly inhibited by methanol extract in continuous cropping soil. Methanol extract from continuous cropping soil was successively fractionated with solvents such as n-hexane, ethyl acetate, n-butanol and water. The seedling growth was inhibited by ethyl acetate fraction obtained in methanol extract.

Keywords

References

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