한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제43권2호
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  • Pages.125-130
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  • 2010
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지
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어류 양식장에서 분리한 Vibrio parahaemolyticus의 Class I Integron에 의한 Trimethoprim 내성

Trimethoprim Resistance by Class I Integron in Vibrio parahaemolyticus from a Fish Farm

  • 유홍식 (국립수산과학원 식품안전과) ;
  • 박큰바위 (국립수산과학원 남서해수산연구소) ;
  • 오은경 (국립수산과학원 식품안전과) ;
  • 이태식 (국립수산과학원 서해수산연구소) ;
  • 신순범 (국립수산과학원 식품안전과) ;
  • 권지영 (국립수산과학원 식품안전과) ;
  • 김지회 (국립수산과학원 남동해수산연구소) ;
  • 손광태 (국립수산과학원식품안전과)
  • Yu, Hong-Sik (Food Safety Division, National Fisheries Research & Development Institute, NFRDI) ;
  • Park, Kunbawui (Southwest Sea Fisheries Research Institute, NFRDI) ;
  • Oh, Eun-Gyoung (Food Safety Division, National Fisheries Research & Development Institute, NFRDI) ;
  • Lee, Tae-Seek (West Sea Fisheries Research Institute, NFRDI) ;
  • Shin, Soon-Bum (Food Safety Division, National Fisheries Research & Development Institute, NFRDI) ;
  • Kwon, Ji-Young (Food Safety Division, National Fisheries Research & Development Institute, NFRDI) ;
  • Kim, Ji-Hoe (Southeast Sea Fisheries Research Institute, NFRDI) ;
  • Son, Kwang-Tae (Food Safety Division, National Fisheries Research & Development Institute, NFRDI)
  • 투고 : 2010.02.10
  • 심사 : 2010.04.13
  • 발행 : 2010.04.30
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초록

A trimethoprim resistant Vibrio parahaemolyticus, which cause acute gastroenteritis in humans, was isolated from farmed fish and seawater. The resistance profiles of isolated V. parahaemolyticus and their correlation with mobile elements were investigated. All of the V. parahaemolyticus were resistance to both rifampin and trimethoprim. The presence of class I integron was confirmed by PCR. PCR-amplified inserted gene cassettes contained aminoglycoside aac6-II, rifampin arr-3 and trimethoprim dfrA27 resistance genes. This study indicated that class I integron mainly contributed to the circulation of trimethoprim resistance determinants in V. parahaemolyticus.

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참고문헌

  1. Acar JF and Goldstein FW. 1991. Disk susceptibility test. In: Antibiotics in Laboratory Medicine, Lorian V, ed. Williams & Wilkins, Baltimore, U.S.A., 17-52.
  2. Bito A and Susani M. 1994. Revised analysis of aadA2 gene of plasmid pSa. Antimicrob Agents Chemother 38, 1172-1175. https://doi.org/10.1128/AAC.38.5.1172
  3. Ceccarelli D, Salvia AM, Sami J, Cappuccinalli P and Colombo MM. 2006. New cluster of plasmidlocated class 1 integrons in νibrio cholerae O1 and a dfrA15 cassette-containing integron in Vibrio parahaemolyticus isolated in Angola. Antimicrob Agents Chemother 50, 2493-2499. https://doi.org/10.1128/AAC.01310-05
  4. Dalsqaard A, Forslund A, Tam NV, Vinh DX and Cam PD. 1999. Cholera in Vietnam: changes in genotypes and emergence of class 1 integrons containing aminoglycoside resistance gene cassettes in Vibrio cholerae O1 strains isolated from 1979 to 1996. J Clin Microbiol 37, 734-741.
  5. Dalsqaard A, Forslund A, Serichantalergs O and Sandvang D. 2000. Distribution and content of class 1 integrons in different Vibrio cholerae O-serotype strains in Thailand. Antimicrob Agents Chemother 44, 1315-1321. https://doi.org/10.1128/AAC.44.5.1315-1321.2000
  6. Fluit AC and Schmitz FJ. 1999. Class 1 integrons, gene cassettes, mobility, and epidemiology. Eur J Microbiol Infect Dis 18, 761-770. https://doi.org/10.1007/s100960050398
  7. Gaskins HR, Collier CT and Anderson DB. 2002. Antibiotics as growth promotants: mode of action. Anim Biothechnol 13, 29-42. https://doi.org/10.1081/ABIO-120005768
  8. Hall RM and Collis CM. 1995. Mobile gene cassettes and integron: capture and spread of genes by site-specific recombination. Mol Microbiol 15, 593-600.
  9. Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ, Kieser HM, Lydiate DJ, Smith CP and Schrempf H. 1985. Genetic manipulation of Streptomyces a laboratory manual. The John Innes Foundation. Norwich, England, 1-356.
  10. Huovinen P, Sundstrom L, Swedberg G and Skold O 1995. Trimethoprim and sulfonamide resistance. Antimicrob Agents Chemother 39, 279-289. https://doi.org/10.1128/AAC.39.2.279
  11. Huovinen P. 2001. Resistance to trimethoprim-sulfamethoxazole. Clin Infect Dis 32, 1608-1614. https://doi.org/10.1086/320532
  12. Iversen J, Sandvang D, Srijan A, Cam PD and Dalsqaard A. 2003. Characterization of antimicrobial resistance, plasmids, and gene cassettes in Shigella spp. from patients in vietnam. Microb Drug Resist 9 suppl 1:S17-24. https://doi.org/10.1089/107662903764736300
  13. Jeong JY, Yoon HJ, Kim ES, Lee Y, Choi SH, Kim NJ, Woo JH and Kim YS. 2005. Detection of qnr in clinical isolates of Escherichia coli from Korea. Antimicrob Agents Chemother 49, 2522-2524. https://doi.org/10.1128/AAC.49.6.2522-2524.2005
  14. Kim SH, Wei CI, Tzon YM and An H. 2005. Multidrugresistant KIebsiella pneumoniae isolated from farm environments and retail products in Oklahoma. J Food Prot 68, 2022-2029. https://doi.org/10.4315/0362-028X-68.10.2022
  15. Lau MM and Ingham Sc. 2001. Survival of faecal indicator bacteria in bovine manure incorporated into soil. Lett Appl Microbiol 33, 131-136 https://doi.org/10.1046/j.1472-765x.2001.00962.x
  16. Navia MM, Ruiz J and Vila J. 2004. Molecular charactenzation of the integrons in Shigella strains isolated from patients with traveler's diarrhea. Diagn Microbiol Infect Dis 48, 175-179. https://doi.org/10.1016/j.diagmicrobio.2003.10.007
  17. Oh EG, Yu HS, Shin SB, Son KT, Park KB, Kwon JY, Lee TS and Lee HJ. 2008. Trimethoprim resistance of νibrio parahaemolyticus isolated from fish farm. J Kor Fish Soc 41 , 324-329.
  18. Recchia GD and Hall RM. 1995. Gene cassette: a new class of mobile element. Microbiology 141, 3015-3027. https://doi.org/10.1099/13500872-141-12-3015
  19. Rosser SJ and Y oung HK. 1999. Identification and characterization of class 1 integrons in bacteria from an aquatic environment. J Antimicrob Chemother 44, 11-18.
  20. Tollefson L, Altekruse SF and Potter ME. 1997. Therapeutic antibiotics in animal feeds and antibiotic resistance. Rev Sci Tech 16, 709-715. https://doi.org/10.20506/rst.16.2.1054
  21. Warren JW, Abrutyn E, Hebel JR, Johnson JR, Schaeffer AJ and Stamm WE. 1999. Guidelines for antimicrobial treatment of uncomplicated acutebacterial cystitis and acute pyelonephritis in women. Clin Infiect Dis 29, 745-758. https://doi.org/10.1086/520427
  22. White PA, Mclver CJ, Deng Y and Rawlinson WD. 2000. Characterization of two new gene cassettes, aadA5 and dfrA17. FEMS Microbiol 182, 265-269. https://doi.org/10.1111/j.1574-6968.2000.tb08906.x
  23. Zhang H, Shi L, Li L, Guo S, Zhang X, Yamasaki X, Miyoshi S and Shinoda S. 2004. Identification and characterization of class 1 integron resistance gene cassettes among Salmonella strains isolated from healthy humans in China. Microbiol Immunol 48, 639-645. https://doi.org/10.1111/j.1348-0421.2004.tb03473.x

피인용 문헌

  1. Antibiotic-Resistance Profiles and the Identification of the Ampicillin-Resistance Gene of Vibrio parahaemolyticus Isolated from Seawater vol.43, pp.6, 2010, https://doi.org/10.5657/kfas.2010.43.6.637
  2. Antimicrobial-resistance Profiles and Virulence Genes of Vibrio parahaemolyticus Isolated from Seawater in the Wando Area vol.47, pp.3, 2014, https://doi.org/10.5657/KFAS.2014.0220