The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Effects of Gaeullijin-Tang on the Progression of the Estradiol Valerate-induced Polycystic Ovaries in Rats

개울이진탕(開鬱二陳湯)이 Estradiol Valerate로 유도된 흰쥐의 다낭성 난소 발달에 미치는 영향

  • Yun, Mun-Hui (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Lee, Dong-Nyung (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Seo, Il-Bock (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Kim, Hyung-Jun (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University)
  • 윤문희 (세명대학교 한의과대학 한방부인과교실) ;
  • 이동녕 (세명대학교 한의과대학 한방부인과교실) ;
  • 서일복 (세명대학교 한의과대학 한방부인과교실) ;
  • 김형준 (세명대학교 한의과대학 한방부인과교실)
  • Received : 2010.04.26
  • Accepted : 2010.05.12
  • Published : 2010.05.31
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Abstract

Purpose: This study was designed to investigate the effects of Gaeullijin-Tang(GIT) on the progression of the estradiol valerate(EV)-induced polycystic ovaries(PCO) in rats. Methods: PCO was induced by single intramuscular injection with estradiol valerate(EV)(4mg) in female rats. Normal group(n=8) were injected with sesame oil and orally administrated distilled water for sixty days. PCO control group (n=8) were injected with EV and orally administrated distilled water for sixty days. GIT treated group(n=8) were injected with EV and orally administrated GIT for same duration. At the end day of experiment, we measured weights of body, ovaries, adrenal glands, uterus and contents of serum androsternedione (ADD) and total estrogen. The histomorphometrical changes of ovaries were also evaluated. And we observed the NGF and CRF expression by immunohistochemistry. Results: The results were as follows - The weights(mg) of ovaries in GIT treated group($75.5{\pm}4.3$) were significantly increased(p<0.001) compared with PCO control group($54.3{\pm}4.5$). - The numbers of mature follicles in GIT treated group($5.3{\pm}1.2$) were significantly increased(p<0.05) compared with PCO control group($3.5{\pm}1.2$). - The numbers of corpora lutea in GIT treated group($1.8{\pm}1.3$) were significantly increased(p<0.05) compared with PCO control group($0.3{\pm}0.7$). Conclusion: From the above results, we concluded that Gaeullijin-Tang(GIT) contributes to stimulating normally ovulation by decreasesing ovarian NGF concentrations.

Keywords

References

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