Korean journal of food and cookery science (한국식품조리과학회지)

Korean Society of Food and Cookery Science (한국식품조리과학회)
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Antioxidant Activity and Neuroprotective Effect of Concentrates from Commercial Sweet Persimmon Wine

시판 단감 와인농축물의 항산화 활성 및 신경세포 보호효과

  • Seo, Hye-Kyung (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Jang, Sun-Young (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Hyun-Jung (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Joong-Hyeop (Malgeunnaeil Co.) ;
  • Ahn, Gwang-Hwan (Sweet Persimmon Research Institute, Gyungsangnamdo Agricultural Research & Extension Services) ;
  • Lee, Seung-Cheol (Department of Food Science and Biotechnology, Kyungnam University)
  • Received : 2009.10.12
  • Accepted : 2010.01.19
  • Published : 2010.02.28
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Abstract

The antioxidant activity of commercial sweet persimmon wine concentrate (SPWC) was evaluated by determining the total phenol content (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing power (RP), and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities. TPC in the SPWC was $9.29{\pm}0.11\;mg$ gallic acid equivalents (GAE)/g, which corresponds to 31.59 mg GAE/100 mL of the wine. The IC50 for the DPPH radical scavenging activity, RP, and ABTS radical scavenging activity of SPWC were 2.96, 1.44, and 0.48 mg/mL, respectively. The neuroprotective effect of SPWC against glutamate-induced neurotoxicity in N18-RE-105 cells was investigated. Treatment of N18-RE-105 cells with various SPWC concentrations under glutamate resulted in the induction of a protective effect in a dose-dependent manner, as determined by the MTT reduction assay. These results suggest that SPWC exhibits considerable antioxidant and neuroprotective activity.

단감 와인을 농축한 후, 농도별로 녹여 추출물을 얻었다. 이 추출물을 이용하여 단감 와인의 항산화 활성을 조사하였다. 단감 와인의 총 페놀 함량(TPC)을 조사한 결과 $9.29{\pm}0.11\;mg/g$의 페놀을 함유하였다. DPPH 라디칼 소거능과 환원력 그리고 ABTS 라디칼 소거능은 농도 의존적으로 활성을 보였으며, $IC_{50}$ 값이 각각 2.959 mg/mL, 1.437 mg/mL 그리고 0.484 mg/mL으로 L-ascorbic acid 보다 낮은 활성을 보였다. 그리고, 단감 와인 농축물은 $10\;{\mu}g/mL$의 농도에서 무처리구에 비해 glutamate 독성으로부터 뇌신경계 세포주 hybridoma N18-RE-105세포를 22% 이상 보호하는 효과를 나타내었다.

Keywords

References

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