Lactobacillus plantarum과 Bifidobacterium longum을 이용한 대두 이소플라본의 비배당체로의 전환

Bioconversion of Soybean Isoflavone by Lactobacillus plantarum and Bifidobacterium longum

  • 김인복 (원광대학교 식품영양학.생활자원개발연구소) ;
  • 신선 (원광대학교 식품영양학.생활자원개발연구소) ;
  • 임병락 ((주)H&BT) ;
  • 성금수 (군산대학교 화학과) ;
  • 이영은 (원광대학교 식품영양학.생활자원개발연구소)
  • Kim, In-Bok (Department of Food and Nutrition.Institute for better living, Wonkwang University) ;
  • Shin, Sun (Department of Food and Nutrition.Institute for better living, Wonkwang University) ;
  • Lim, Byung-Lak (Human and Biotechnology Co., Ltd.) ;
  • Seong, Gem-Soo (Department of Chemistry, Gunsan University) ;
  • Lee, Young-Eun (Department of Food and Nutrition.Institute for better living, Wonkwang University)
  • 투고 : 2010.03.19
  • 심사 : 2010.04.14
  • 발행 : 2010.04.30

초록

In this study, phytoestrogen for the industrial production of soybean probiotics by lactic acid bacteria (LAB) was studied in a soybean extract. Soybean was fermented with LAB, Lactobacillus plantarum KCTC 3108 and Bifidobacterum longum ATCC 15707. The change in the content of various isoflavones (aglycone and glucoside) and the $\beta$-glucosidase activity in soybean during fermentation were investigated and shown to be dependent on the starter organism. Soybean extract powder fermented with L. plantarum showed the highest $\beta$-glucosidase activity and the greatest increase in the aglycone content. After 48h of fermentation, the contents of daidzin, genistin and glycitin in L. plantarum decreased from a mean initial levels of $83.03{\pm}2.17$, $168.13{\pm}8.17$ and $20.02{\pm}1.07$, respectively, to mean levels of $5.34{\pm}3.24$, $3.79{\pm}0.57$ and $1.87{\pm}1.09\;mg$/100 g. Whereas, after 48h fermentation, the contents of daidzein, genistein and glycitein increased from a mean initial levels of $8.09{\pm}0.78$, $11.20{\pm}0.84$ and $4.71{\pm}0.46$, respectively, to mean levels of $85.76{\pm}0.84$, $175.87{\pm}2.21$ and $22.41{\pm}0.91\;mg$/100 g. Taken together, these results suggested an increase of aglycones and decrease of glucoside in isoflavones occurred during fermentation, which coincided with an increase of $\beta$-glucosidase activity in the fermented soybean extract powder.

키워드

참고문헌

  1. 이영은, 홍승헌. 2003. 한방식품재료학. (주)교문사. 서울. pp 42-44
  2. 정동효. 2005. 대두이소플라본. 신일상사. 서울. pp 3-4, p 65
  3. AOAC. 1990. Official methods analysis of the association of official analytical chemists 15th ed. The association of official analytical chemists Inc. Virginia. USA.
  4. Adlercreutz H. 1995. Phytoestrogens: Epiemiology and a possible role in cancer protection. Environ Health Perspect 103(7): 103-112 https://doi.org/10.1289/ehp.95103s7103
  5. Anthony MS, Clarkson TB, Williams JK. 1998. Effects of soy isoflavones on atherosclerosis: potential $mechanism^{1-3}$. Am J Clin Nutr 68(6):1390S-1393S
  6. Choi YB, Woo JG, Noh WS. 1999. Hydrolysis of $\beta-glucosidic$ bonds of isoflavone conjugates in the lactic acid fermentation of soy milk. Korean J Food Sci Technol 31(1):189- 195
  7. Clarkson TB, Anthony MS, Hughes CL, Jr. 1995. Estrogenic soybean isoflavones and chronic disease risks and benefits. Trends Endocrinol Metab 6(1):11-16 https://doi.org/10.1016/1043-2760(94)00087-K
  8. Esaki H, Watanabe R, Hishikawa N, Osawa T, Kawakishi S. 2004. Utility of isoflavone preparations from soy sauce cake as antioxidant materials. J Jpn Soc Food Sci Technol 51(1): 47-53 https://doi.org/10.3136/nskkk.51.47
  9. Fukutake M, Takahshi M, Ishida K. 1996. Quantification of genistein and genistin in soybeans and soybean products. Food Chem Toxicol 34(5):457-461 https://doi.org/10.1016/0278-6915(96)87355-8
  10. Izumi T, Piskula MK, Osawa S, Obata A, Tobe K, Saito M, Kataoka S, Kubota Y, Kikuchi M. 2000. Soy isoflavone aglycones are absorbed faster and in higher amounts than their glucosides in humans. J Nutr 130(7):1695-1699
  11. Kim JH, Lee BR, Moo YP. 1998. Overproduction and secretion of $\beta-glucosidase$ in Bacillus subtilis. J Microbiol Biotechnol 8(2):141-145
  12. Peterson G, Barnes S. 1991. Genistein inhibition of the growth of human breast cancer cells: Independence from estrogen receptors and the multi-drug resistance gene. Biochem Biophys Res Comm 179(1):661-667 https://doi.org/10.1016/0006-291X(91)91423-A
  13. Park KB, Oh SH. 2007. Production of yogurt with enhanced levels of gamma-aminobutyric acid and valuable nutrients using lactic acid bacteria and germinated soybean extract. Bioresource Technol 98(8):1675-1679 https://doi.org/10.1016/j.biortech.2006.06.006
  14. Setchell KDR, Cassidy A. 1999. Dietary isoflavones: biological effects and relevance to human health. J Nutr 129(3):758- 767
  15. Shigemitsu K, Makoto S, Takashi I, Teiji U, Kazuyoshi O. 1991. A new isoflavone glycoside in soybean seed (glycine max merrill), glycitein $7-O-\beta-D$-(6"-Ο-acetyl)-glucopyranoside. Agric Biol Chem 55(3):859-860 https://doi.org/10.1271/bbb1961.55.859
  16. Shimakawa Y, Matsubara S, Yuki N, Ikeda M, Ishikawa F. 2003. Evaluation of bifidobacterium breve strain Yakult-fermented soymilk sa a probiotic food. Int J Food Microbiol. 81(2): 131-136 https://doi.org/10.1016/S0168-1605(02)00224-6
  17. Tsangalis D, Ashton JF, McGill AEJ, Shah NP. 2002. Enzymic transformation of isoflavone phytoestrogens in soymilk by $\beta-glucosidase$ producing bifidobacteria. J Food Sci 67(8): 3104-3113 https://doi.org/10.1111/j.1365-2621.2002.tb08866.x
  18. Wang HJ, Murphy PA. 1994. Isoflavone content in commercial soybean foods. J Agric Food Chem 42(8):1666-1673 https://doi.org/10.1021/jf00044a016
  19. Xu X, Wang HJ, Murphy PA, Cook L, Hendrich S. 1994. Daidzein is a more biovailable soymilk isoflavone than is genistein in adult woman. J Nutr 124(6):825-832
  20. Yu JH, Lew ID, Park CK, Lim HC. 1988. Lactic acid fermentation in soymilk by single and mixed cultures of lactobacillus casei and kluyveromyces fragilis. Korean J Food Sci Technol 20(4):518-525