Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Monitoring of Aflatoxins in Medicinal Herbs

유통 생약재의 아플라톡신 모니터링

  • 김용훈 (서울지방식품의약품안전청) ;
  • 강한샘 (서울지방식품의약품안전청) ;
  • 오선우 (서울지방식품의약품안전청) ;
  • 이화정 (식품의약품안전청) ;
  • 김미경 (식품의약품안전청) ;
  • 정소영 (서울지방식품의약품안전청) ;
  • 최선희 (서울지방식품의약품안전청) ;
  • 방수진 (서울지방식품의약품안전청) ;
  • 한경진 (서울지방식품의약품안전청) ;
  • 이지원 (서울지방식품의약품안전청) ;
  • 김영선 (서울지방식품의약품안전청) ;
  • 김희연 (서울지방식품의약품안전청)
  • Received : 2009.09.14
  • Accepted : 2009.10.24
  • Published : 2010.02.28
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Abstract

This study was conducted to monitor aflatoxins in various medicinal herbs, providing available data for the safety of those products. To monitor aflatoxins in medicinal herbs, a total of 400 samples of 40 different herbs were collected in commercial retailers in Seoul, Daejeon, Gwangju, Daegu, and Busan from March to August, 2008. The samples that passed the sensory evaluation were tested for aflatoxins. Aflatoxins in samples were analyzed by HPLC-florescence coupled with photochemical enhancement. Samples were extracted with 70% methanol and then diluted to the appropriate concentration. A refining process was performed using an immunoaffinity column. The analytical method used in this study was validated. The $R^2$ value for aflatoxin $B_1$ was 0.99946, and the detection range was from 0.25 to 10.0 ng/mL. The accuracy of the analysis was ranged from 83.2% to 101.8%. The relative standard deviation (RSD) in the aflatoxin $B_1$ analysis was 3.4%, demonstrating the precision of this method. In addition, the detection limit and quantitative analysis limit of aflatoxin $B_1$ was $0.53\;{\mu}g/kg$ and $1.76\;{\mu}g/kg$, respectively. These results indicated that the analytical method used in this study was appropriate. The results of HPLC showed that 1% (4 samples) of the samples may contain aflatoxins. The concentration of quantified aflatoxin was $2.3\;{\mu}g/kg$ for both Quisqualis fructus and Remotiflori radix samples. The other samples were below the limit of quantification. Moreover, the concentration of aflatoxin $B_1$ which is made by specific fungi were below the level of regulation. Only 20% of aflatoxin $B_1$ were transferred to hot water. Therefore, the levels of aflatoxins in medicinal herbs were considered to be safe especially considering the aflatoxin transfer ratio.

고시된 아플라톡신 시험법의 정량한계, 검출한계를 개선한 분석법을 유효화하였으며, 개선한 분석법으로 강활, 개자 등의 생약 400개의 시료를 분석하였다. 사군자와 제니 각 1건에서 아플라톡신 $B_1$이 각 $2.3\;{\mu}g$/kg 검출되었으며, 사군자와 대풍자 1건에서 정량한계 미만의 아플라톡신이 검출되었다. 탕제로의 이행률은 아플라톡신 $B_1$의 경우 약 20% 정도의 이행률을 나타냈다. 아플라톡신 $B_1$ 기준치보다 낮게 검출되어 현재까지는 아플라톡신으로부터 안전한 것으로 판단되었다.

Keywords

References

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