Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Development of Molecular Markers for Alternative Oxidase Synthesis Genes in Brassica rapa L.

배추 alternative oxidase 합성 유전자와 연관된 분자마커 개발

  • Jeong, Ye-Sol (Department of Life Science, Dongguk University-Seoul) ;
  • Chung, Sang-Min (Department of Life Science, Dongguk University-Seoul)
  • 정예솔 (동국대학교-서울 생명과학과) ;
  • 정상민 (동국대학교-서울 생명과학과)
  • Received : 2009.11.16
  • Accepted : 2009.11.27
  • Published : 2010.02.28
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Abstract

The low and high temperature stress might affect the yield and quality of many crop species. Alternative oxidase (AOX) gene is known as factors related to stress resistance in plants. In order to develop molecular markers related to stress resistance in Chinese cabbage, fifteen ESTs sharing sequence similarity to arabidopsis AOX genes were found using Brassica rapa EST database from NCBI. The polymorphic DNA sequences using the ESTs were then screened between Chinese cabbage, 'Chiifu' and 'Kenshin'. We found four ESTs that have either insertion or deletion between the two cultivars. These polymorphic sites were then targeted for development of the four PCR based molecular markers. These molecular markers developed in this study could be useful for a test of their relationship with abiotic stress resistance in Chinese cabbage.

작물의 수량과 품질은 저온 및 고온 스트레스에 영향을 받는 것으로 알려져 있다. 본 연구에서는 배추에서의 스트레스 저항성과 연관된 분자마커를 개발하기 위하여 저온에서의 스트레스 저항성에 영향을 미치는 것으로 알려져 있는 alternative oxidase (AOX) 합성 유전자 관련 분자 마커를 개발하였다. 총 15개의 AOX 합성 유전자와 관련된 Brassica rapa ESTs를 arabidopsis AOX 합성 유전자 염기서열을 이용하여 찾을 수 있었다. 이를 이용하여 고온에서 상대적으로 약한 '지부'품종과 상대적으로 강한 '권심' 사이에서 DNA 염기서열을 조사하여 4개의 ESTs에서 insertion 또는 deletion을 찾았고 PCR로 확인 가능한 4개의 공동우성 마커를 개발하였다. 본 연구에서 개발된 분자마커는 배추 작물에서 환경스트레스 저항성과 유전적 연관성을 확인하는데 유용하게 사용될 수 있다고 기대된다.

Keywords

References

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