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식물류 혼합물과 마늘의 복합 조성이 고콜레스테롤혈증 흰쥐의 혈청 지질에 미치는 영향

Effect of the Plants Mixture and Garlic Composition on Serum Lipid Level of Hypercholesterolemic Rats

  • Shin, Jung-Hye (Namhae Garlic Research Institute) ;
  • Lee, Soo-Jung (Department of Food Science and Nutrition, Gyeongsang National University) ;
  • Jung, Woo-Jae (Department of Food Science and Nutrition, Gyeongsang National University) ;
  • Seo, Jong-Kwon (Division of Food Science, International University of Korea) ;
  • Sung, Nak-Ju (Namhae Garlic Research Institute)
  • 투고 : 2009.12.15
  • 심사 : 2010.01.14
  • 발행 : 2010.03.30

초록

식물류 혼합물 1%와 0.3, 0.5% 및 0.7%의 마늘 추출물을 고콜레스테롤혈증 유발 흰쥐에 4주간 실험 급이한 후 혈액 내 지질성분에 미치는 영향을 분석하였다. 마늘 추출물의 첨가농도를 0.3~0.7%로 달리하였을 때 총 식이 섭취량은 정상군에 비해 실험군에서 유의적으로 높았으나 실험군 간에는 차이가 없었다. 총 콜레스테롤은 식물류 혼합물 및 마늘 추출물을 급이함으로써 대조군에 비해 약 20%, 중성지방은 9.3~15.0% 정도 감소되었으며, 인지질도 유사한 경향이었다. HDL-콜레스테롤은 대조군과 실험군간의 유의차가 없었으며, LDL-콜레스테롤은 정상군에 비해 대조군에서 9.4배 더 높았는데, 마늘 추출물을 0.5% 및 0.7% 첨가 급이할 경우 각각 $68.45{\pm}12.83\;mg/dl$$66.35{\pm}5.18\;mg/dl$로 유의적으로 감소하였다. VLDL-콜레스테롤은 마늘 추출물을 0.5% 이상 첨가 급이할 경우 정상군과 동일한 수준까지 저하되었다. 동맥경화 지수 및 심혈관질환 위험지수도 식물류 혼합물과 마늘 추출물의 급이군에서 유의적으로 낮았다. 혈당은 마늘 추출물 0.5% 첨가군에서 $189.37{\pm}12.02\;mg/dl$로 가장 낮았고, 총 단백질 함량은 식물류 혼합물과 마늘 추출물 급이시 $9.56{\pm}0.87{\sim}10.05{\pm}2.69\;mg/dl$로 정상군에 비해 유의적으로 증가되었다. GOT 활성은 마늘추출물의 0.3% 첨가군에서 $81.86{\pm}11.73\;u/ml$로 대조군에 비해 유의적인 감소를 보였고, GPT 활성은 식물류 혼합물 및 마늘 추출물 급이군과 대조군간에 유의차가 없었다. TBARS 함량은 마늘 추출물을 0.7%로 급이한 군에서 정상군보다 낮은 함량이었다. 혈청의 항산화 활성도 마늘 추출물의 0.7% 급이군에서 $83.75{\pm}2.32%$로 대조군에 비해 항산화 활성이 유의적으로 높았다.

This study was designed to investigate the effects of 4 kinds of plant water extract mixture and garlic extract (PMC) administration on serum lipid metabolism in hypercholestrolemic rats. The normal group was administered a cholesterol free diet, the control group a 1% cholesterol diet, and each experimental group was given a diet of 1% cholesterol, 1% plant mixture and 0.3, 0.5, 0.7% garlic extract (PMC-I, PMC-II, PMC-III), respectively. Each diet was administered orally to SD-male rats for 4 weeks. Total cholesterol content decreased by about 20% with administration of PMC. Triglyceride content also decreased from 9.3 to 15.0% compared to the control group, and phospholipid was similar to triglyceride. There was no significant difference in HDL-cholesterol content between the control and experimental groups. LDL-cholesterol content of the normal group was 9.4 times lower than the control group and its content was significantly lower in the PMC-II ($68.45{\pm}12.83\;mg/dl$) and PMC-III ($66.35{\pm}5.18\;mg/dl$) groups than the PMC-I group. VLDL-cholesterol content of the PMC-II and III groups were similar to the normal group. Atherogenic index (AI) and cardiac risk factor (CRF) were significantly lower in the PMC group. Blood glucose content was the lowest in the PMC-II ($189.37{\pm}12.02\;mg/dl$) group among all groups tested. Total protein content was $9.56{\pm}0.87{\sim}10.05{\pm}2.69\;mg/dl$ in the PMC-I~III groups and was significantly higher than the normal group. CPT activity did not show a significant difference among the experimental groups, while COT activity was effective only in the PMC-I group. Serum TBARS content in the PMC-III group was lower than in the normal group. Serum antioxidant activity by DPPH radical scavenging was $83.75{\pm}2.32%$ in the PMC-III group, which was significantly higher than the control group.

키워드

참고문헌

  1. Banerjee, S. K., M. Maulik, S. C. Mancahanda, A. K. Dinda, S. K. Gupta, and S. K. Maulik. 2002. Dose-dependent induction of endogenous antioxidants in rat heart by chronic administration of garlic. Life Sci. 70, 1509-1518. https://doi.org/10.1016/S0024-3205(01)01514-4
  2. Chang, M. L. and M. A. Johnson. 1980. Effect of garlic on carbohydrate metabolism and lipid synthesis in rats. J. Nutr. 110, 931-936.
  3. Cheung, P. C. K. 1998. Plasma and hepatic cholesterol levels and fecal neutral sterol excretion are altered in hamsters fed straw mushroom diets. J. Nutr. 128, 1512-1516.
  4. Chi, M. S., E. T. Koh, and T. J. Stewart. 1982. Effect of garlic on lipid metabolism in rats fed cholesterol or lard. J. Nutr. 112, 241-248.
  5. Choi, H. S., J. O. Ha, M. H. Choo, M. S. Na, and M. Y. Lee. 2004. Effect of Bambusae caulis in liquamen on lipid metabolism in rats fed high fat diet. Korean J. Food Preserv. 11, 373-382.
  6. Chun, H. J. and J. E. Paik. 1997. Effect of heart treatment of garlic added diet on the blood of spontaneously hypertension rat. J. Korean Soc. Food Sci. Nutr. 26, 103-108.
  7. Cook, N. C. and S. Samman. 1996. Flavonoids-chemistry, metabolism, cardiovascular effects and dietary sources. J. Nutr. Biochem. 7, 66-76. https://doi.org/10.1016/0955-2863(95)00168-9
  8. Dietschy, J. M. and J. D. Wilson. 1970. Regulation of cholesterol metabolism. New Engl. J. Med. 282, 1179-1183. https://doi.org/10.1056/NEJM197005212822105
  9. Elkayam, A., D. Mirelman, E. Peleg, M. Wilchek, T. Miron, A. Rabinkov, M. Oron-Herman, and T. Rosenthal. 2003. The effects of allicin on weight in fructose-induced hyperinsulinemic, hyperlipidemic, hypertensive rats. Am. J. Hypertens. 16, 1053-1056. https://doi.org/10.1016/j.amjhyper.2003.07.011
  10. Focke, M., A. Feld, and K. Lichtenthaler. 1990. Allicin, a naturally occurring antibiotic from garlic, specially inhibits acetyl CoA synthetase. FEBS Letters 261, 106-108. https://doi.org/10.1016/0014-5793(90)80647-2
  11. Friedewald, W. T., R. I. Levy, and D. S. Fredrickson. 1972. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrefuge. Clin. Chem. 18, 499-502.
  12. Haenen, G. R., J. B. Paquay, R. E. Korthouwer, and A. Bast. 1997. Peroxynitrite scavenging by flavonoids. Biochem. Bioph. Res. Co. 236, 591-593. https://doi.org/10.1006/bbrc.1997.7016
  13. Haglund, O., R. Loustarinen, R. Wallin, I. Wibell, and T. Saldeen. 1991. The effect of oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in man supplemented with vitamin. Eur. J. Nutr. 121, 165-172.
  14. http://www.nso.go.kr. 2007. 2007 Annual report on the cause of death statistics. Korea National Statistical Office.
  15. Kang, M. J., S. J. Lee, J. H. Shin, S. K. Kang, J. G. Kim, and N. J. Sung. 2008. Effect of garlic with different processing on lipid metabolism in 1% cholesterol fed rat. J. Korean Soc. Food Sci. Nutr. 37, 162-169. https://doi.org/10.3746/jkfn.2008.37.2.162
  16. Kang, S. M., J. Y. Shim, S. J. Hwang, S. G. Hong, H. E. Jang, and M. H. Park. 2003. Effects of Saengshik supplementation on health improvement in diet-induced hypercholesterolemic rats. J. Korean Soc. Food Sci. Nutr. 32, 906-912. https://doi.org/10.3746/jkfn.2003.32.6.906
  17. Kim, Y. H., J. H. Lee, B. K. Koo, and H. S. Lee. 2007. Isoflavone-rich bean sprouts improves hyperlipidemia. J. Korean Soc. Food Sci. Nutr. 36, 1248-1256. https://doi.org/10.3746/jkfn.2007.36.10.1248
  18. Koh, J. B. 2006. Effects of cheonggukjang added Phellinus linteus on lipid metabolism in hyperlipidemic rats. J. Korean Soc. Food Sci. Nutr. 35, 410-415. https://doi.org/10.3746/jkfn.2006.35.4.410
  19. Lee, J. J., M. H. Cha, and M. Y. Lee. 2006. Effect of Pimpinella brachycarpa extract on lipid metabolism in rats fed high cholesterol diet. J. Korean Soc. Food Sci. Nutr. 35, 1151-1158. https://doi.org/10.3746/jkfn.2006.35.9.1151
  20. Lee, Y. C. 1991. Hypercholesterolemia in Korea and nutritional factors. Korea Soc. Lipidol. Atherosc. 1, 111-122.
  21. Lim, B. O., T. W. Seo, H. M. Shin, D. K. Park, S. U. Kim, K. H. Cho, and H. C. Kim. 2000. Effect of Betulae Platyphyllae Cortex on free radical in diabetic rats induced by streptozotocin. Korea J. Herbology 15, 69-77.
  22. McGill, H. C. 1979. The relationship of dietary cholesterol to serum cholesterol concentration and to atherosclerosis in man. Am. J. Clin. Nutr. 32, 2664-2702.
  23. Nam, K. H., H. W. Baik, T. Y. Choi, S. G. Yoon, S. W. Park, and H. J. Joung. 2007. Effects of ethanol extract of onion on the lipid profiles in patients with hypercholesterolemia. Korean J. Nutr. 40, 242-248.
  24. Saifer, A. and N. I. Feldman. 1971. The photometric determination of gangliosides with the sulfo-phospho vanillin reaction. J. Lipid Res. 12, 112-115.
  25. Seo, H. J. 1999. Effects of garlic on the blood lipids and other serum components in rats. J. Korean Soc. Food Sci. Nutr. 28, 1339-1348.
  26. Shin, S. H. and M. K. Kim. 2004. Effect of dried powders or ethanol extracts of garlic flesh and peel on antioxidative capacity in 16-month-old rats. Korean J. Nutr. 37, 633-644.
  27. Sodhi, H. S. 1975. Cholesterol metabolism in man, pp. 29-107, In Kritchevsky, D. (ed.), Hypolipidemic agents. Springer-Verlag, Berlin, Germany.
  28. Spady, D. K. and L. A. Woollett. 1993. Regulation of plasma LDL-cholesterol and fatty acid. Annu. Rev. Nutr. 13, 355-381. https://doi.org/10.1146/annurev.nu.13.070193.002035
  29. Yagi, K. 1984. Assay for blood plasma or serum. pp. 328-331, In Method in enzymology. Vol. 105, Academic Press, New York, USA. https://doi.org/10.1016/S0076-6879(84)05042-4
  30. Yu, Y. Y. and M. Y. Shaw. 1994. Garlic reduces plasma lipids by inhibiting hepatic cholesterol and triacylglycerol synthesis. Lipids 29, 189-193. https://doi.org/10.1007/BF02536728

피인용 문헌

  1. Anti-Obesity Effect of Red Garlic Composites in Rats Fed a High Fat-Cholesterol Diet vol.22, pp.5, 2012, https://doi.org/10.5352/JLS.2012.22.5.671
  2. Effects of Rosa multiflora and Rosa multiflora Complex on Lipid Content in Rats Fed a High-Fat·High-Cholesterol Diet vol.44, pp.6, 2015, https://doi.org/10.3746/jkfn.2015.44.6.791
  3. Effects of Garlic Shoot Extract on Lipid Metabolism in Hyperlipidemic Rats Fed a High-fat Diet vol.25, pp.3, 2015, https://doi.org/10.5352/JLS.2015.25.3.276
  4. Effect of Red Garlic-Composites on the Fecal Lipid Level and Hepatic Antioxidant Enzyme Activity in Rats Fed a High Fat-Cholesterol Diet vol.42, pp.1, 2013, https://doi.org/10.3746/jkfn.2013.42.1.017