Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Purification and Identification of Inhibitory Compounds on Helicobacter pylori from Cheongmoknosang Callus for Biomass

Biomass를 위해 제조한 청목노상 callus로부터 Helicobacter pylori 억제물질의 분리 및 동정

  • 윤동혁 (경북대학교 식품공학과) ;
  • 차원섭 (경북대학교 식품공학과) ;
  • 이선호 (안동대학교 해양바이오산업연구소) ;
  • 안봉전 (대구한의대학교 화장품약리학과) ;
  • 김정환 (엔아이피 바이오텍) ;
  • 천성숙 (엔아이피 바이오텍) ;
  • 배종호 (대구미래대학 제과데코레이션과) ;
  • 조영제 (경북대학교 식품공학과)
  • Received : 2009.12.02
  • Accepted : 2009.12.20
  • Published : 2010.03.30
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Abstract

The objective of this research was to evaluate the inhibitory activities of phenolic compounds isolated from Cheomoknosang callus on Helicobacter pylori. Total phenolic compounds of 80% ethanol extracts from callus were 15.3 mg/g. The activity of H. pylori inhibition at 80% ethanol extracts from Cheongmoknosang callus was determined as 14 mm clear zone. Isolation of inhibitory compounds was carried out on Sephadex LH-20 and MCI-gel CHP-20 column chromatography using a gradient elution procedure of increasing MeOH in $H_2O$. The chemical structure of the inhibitory compound against Helicobacter pylori was confirmed as protocatechuic acid, chlorogenic acid, caffeic acid and rosemarinic acid by spectroscopic analysis of FAB-MS, NMR and IR spectrum.

청목노상 잎 시료로부터 spot 배양 후 bioreactor에서 배양된 청목노상 뽕잎callus 추출물을 이용하여 H. pylori 균에 대한 항균활성을 측정한 결과, 높은 항균활성을 나타내는 것을 알 수 있었다. 배양된 청목노상 뽕잎 callus 추출물의 H. pylori 균에 대한 항균활성 물질을 정제하여 $^1H$-NMR, $^{13}C$-NMR, IR 및 FAB-MS spectrum 등을 활용하여 구조분석 한 결과 protocatechuic acid, chlorogenic acid, caffeic acid 및 rosemarinic acid로 동정하였으며, 단일물질 보다 혼합물질에 의한 synergy 효과에 의해 H. pylori 균에 대한 항균활성이 나타나는 것으로 판단되었다.

Keywords

References

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