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Oleanolic acid(OA)의 Streptococcus mutans에 대한 저해효과

Identification of Inhibitory Effect on Streptococcus mutans by Oleanolic Acid

  • 윤요한 (한국원자력연구원 정읍 방사선과학연구소 식품생명공학연구실) ;
  • 최경희 (원광대학교 치과대학 구강미생물학교실)
  • Yoon, Yo-Han (Team for Radiation Food Science & Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Choi, Kyoung-Hee (Department of Oral Microbiology, College of Dentistry, Wonkwang University)
  • 투고 : 2009.11.23
  • 심사 : 2010.02.18
  • 발행 : 2010.03.30

초록

구강에 상주하는 미생물 중, Streptococcus mutans는 충치 및 치주염의 원인인 치아플라그를 형성하는데 중요한 역할을 한다. Phytochemical은 식물에서 추출된 화학성분으로서, 사람의 건강에 유익한 영양물질로서 많은 연구들이 진행되어왔다. 본 연구는 이 phytochemical이 중요 구강미생물인 S. mutans에 대한 효과를 살펴보았다. 최근에 Thomson seedless raisin에서 여러 phytochemicals가 추출되었는데, 그 중 oleanolic acid (OA)와 5-(hydroxymethyl)-2-furfural (HF)의 임상적으로 중요한 여러 미생물에 대한 항균활성효과를 확인한 결과, OA가 그람음성균들에게는 항균활성효과가 나타나지 않았고, 그람양성균들에만 항균활성효과를 보였다. 그러나, HF의 경우에는 모든 균주에 대해 항균활성을 나타내지 않았다. 또한, OA는 S. mutans 균주의 표면부착과 생균막의 형성을 저해하기도 하였다. 따라서, 이 연구결과들은 OA가 치아에 존재하는 S. mutans의 생육 및 여러 생리적 특성들을 저해하므로 항플라그제나 항충치약으로서의 활용가능성을 확인할 수 있었다.

Among endogenous oral microflora, Streptococcus mutans plays a critical role in dental plaque formation, which mainly contributes to the development of caries and periodontal disease. Phytochemicals are plant-derived chemical compounds that have been studied as beneficial nutrients to human health. The purpose of this study was to determine the effects of phytochemicals against S. mutans. Among them, oleanolic acid (OA) and 5-(hydroxymethyl)-2-furfural (HF) from Thomson seedless raisins were tested for anti-microbial effects against various clinically important bacteria. OA inhibited the growth of Gram-positive bacteria, but not Gram-negative bacteria. However, HF did not display any antibacterial effect against any of the strains tested. OA also exhibited inhibitory effects in surface adherence and biofilm formation of S. mutans. The results suggest that OA can be utilized as a potential anti-plaque and anti-caries agent by controlling the physiological characteristics of S. mutans on teeth.

키워드

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피인용 문헌

  1. Antimicrobial Action of Oleanolic Acid on Listeria monocytogenes, Enterococcus faecium, and Enterococcus faecalis vol.10, pp.3, 2015, https://doi.org/10.1371/journal.pone.0118800