The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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New Multi-herb Mixture, HT005, Induces Longitudinal Bone Growth in Male Rats

신한약복합물 HT005의 성장기 흰쥐 장골길이 성장에 미치는 효과

  • Kim, Mi-Yeon (Department of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Ramirez, Marcus J. (Department of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Dong-Hun (Department of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Lim, Dong-Wook (Department of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Choi, Ho-Young (Department of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Ho-Cheol (Department of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University)
  • 김미연 (경희대학교 한의과대학 본초학교실) ;
  • 마르쿠스 라미레즈 (경희대학교 한의과대학 본초학교실) ;
  • 이동헌 (경희대학교 한의과대학 본초학교실) ;
  • 임동욱 (경희대학교 한의과대학 본초학교실) ;
  • 최호영 (경희대학교 한의과대학 본초학교실) ;
  • 김호철 (경희대학교 한의과대학 본초학교실)
  • Received : 2010.02.24
  • Accepted : 2010.03.22
  • Published : 2010.03.30
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Abstract

Objectives : It was investigated new herbal prescription HT005 supported by traditional Korean medicine has a activity on the longitudinal bone growth of rats. HT005 were consisted of the root of Angelica sinensis, the fruit of Alpinia oxyphylla, the root of Astragalus membranaceus, the stem of Eleutherococcus senticosus, and the root of Dioscorea japonica, and Poria cocos. Method : To investigate the effect on longitudinal bone growth in adolescent male Sprague-Dawley rats, the longitudinal length of growth plate was measured directly by the tetracycline fluorescent labeling method and chondrocyte staining method. HT005 administered orally for four days, and the tetracycline was injected twice on the growth plate of the animals for fluorescent dying. The rate of longitudinal bone growth was measured the length between the tetracycline bands which fixed on the 3rd day and 5th day after injection. Cresyl violet was also used to stain the chondrocytes in the growth plate. The length of growth plate after administration was compared. Expression of IGF-1 and BMP-2 in the growth plate was investigated by immunohistochemistry. Results : HT005 showed a significant longitudinal bone growth which was $301.0{\pm}6.1\;{\mu}m/day$ at the dose of 100 mg/kg and $283.8{\pm}1.25\;{\mu}m/day$ (p < 0.001)at the dose of 10 mg/kg of HT005, compared to control group by the tetracycline fluorescent labeling method. HT005 showed a significant chondrocyte length on growth plate which was measured $797.19{\pm}3.31\;{\mu}m$ (p < 0.001) at the dose of 100 mg/kg and $720.14{\pm}2.19$ (p < 0.001) ${\mu}m$ at the dose of 10 mg/kg compared to the control group by cresyl-violet staining method. Both the number and intensity of BMP-2 and IGF-1 positive cells were increased in the hypertrophic zone of growth plate. There was a significant correlation between BMP-2 and IGF-1 expression and heights of chondrocytes in growth plate. Conclusions : Treatment of HT005 on Sprague-Dawley rats markedly increased the longitudinal bone growth. Therefore, HT005 may be an alternative herbal source to growth hormone as it promotes bone growth in children afflicted by growth retardation.

Keywords

References

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