대한본초학회지 (The Korea Journal of Herbology)

  • 제25권1호
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  • Pages.65-74
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  • 2010
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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우슬의 파골세포 분화 억제와 골 흡수 억제효과

Inhibitory Effects of Achyranthis Bidentatae Radix on Osteoclast Differentiation and Bone Resorption

  • 김주호 (원광대학교 약학대학 한약학과.원광한약연구소) ;
  • 기지예 (원광대학교 약학대학 한약학과.원광한약연구소) ;
  • 안지영 (원광대학교 약학대학 한약학과.원광한약연구소) ;
  • 박혜정 (원광대학교 약학대학 한약학과.원광한약연구소) ;
  • 김현주 (원광대학교 약학대학 한약학과.원광한약연구소) ;
  • 곽한복 (원광대학교 의과대학 해부학교실.의과학연구소) ;
  • 오재민 (원광대학교 의과대학 해부학교실.의과학연구소) ;
  • 김윤경 (원광대학교 약학대학 한약학과.원광한약연구소)
  • Kim, Ju-Ho (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Ki, Ji-Ye (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Ann, Ji-Young (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Park, Hye-Jung (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kim, Hyun-Ju (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kwak, Han-Bok (Department of Anatomy, School of Medicine, and Wonkwang Medical Science Institute, Wonkwang University) ;
  • Oh, Jae-Min (Department of Anatomy, School of Medicine, and Wonkwang Medical Science Institute, Wonkwang University) ;
  • Kim, Yun-Kyung (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University)
  • 투고 : 2010.02.20
  • 심사 : 2010.03.22
  • 발행 : 2010.03.30
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초록

Objectives : Achyranthis Bidentatae Radix (ABR) has been used for treating of many symptoms especially osteoporosis and rheumatoid arthritis. In this study, we determined the effects of water extract of ABR in RANKL (Receptor Activator for Nuclear Factor $\kappa$ B Ligand)-induced osteoclast differentiation culture system. Methods : We assayed mRNA expression levels of NFATc1, c-Fos, TRAP, OSCAR, $FcR{\gamma}$, DAP12 and GAPDH in bone marrow macrophages (BMMs) treated with ABR. The protein expression levels of NFATc1, c-Fos, MAPKs and $\beta$-actin in cell lysates treated with ABR were analysed by Western blotting. In addition we determined the effects of water extract of ABR on LPS-induced bone-loss mouse. Results : Water extract of ABR showed remarkable inhibition on RANKL-treated osteoclast differentiation without cytotoxicity. ABR down-regulated the induction of c-Fos and NFATc1 by RANKL. ABR suppressed phosphorylation of JNK, p38 and I-${\kappa}B$. ABR rescued bone erosion by LPS induction in vivo study. Conclusions : These results demonstrate that ABR may be a useful remedy for curing of bone-loss disease such as osteoporosis.

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