The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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Effect of Yukmijihwang-Tang Gamibang Aqua-acupuncture on the Recovery of Chondrocyte Phenotype

육미지황탕가미방(六味地黃湯加味方) 약침이 연골세포에 미치는 영향

  • Choi, Won-Joo (Dongguk Univ-Seoul, Graduate School of Oriental Medicine) ;
  • Min, Sang Yeon (Dongguk Univ-Seoul, Graduate School of Oriental Medicine) ;
  • Kim, Jang Hyun (Dongguk Univ-Seoul, Graduate School of Oriental Medicine)
  • 최원주 (동국대학교-서울 대학원 한의학과) ;
  • 민상연 (동국대학교-서울 대학원 한의학과) ;
  • 김장현 (동국대학교-서울 대학원 한의학과)
  • Received : 2010.04.02
  • Accepted : 2010.04.24
  • Published : 2010.04.30
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Abstract

Objectives The purpose of this study is to examine the effect of chondrocyte re-differentiation in using ukmijihwang-Tang gamibang aqua-acupuncture. Methods In this study, chondrocytes were extracted from New Zealand white rabbit's knee joint, and cultured in monolayer after collagenase treatment. In the third passage, after the mRNA transcript of the type II collagen significantly reduced, diluted Yukmijihwang-Tang gamibang were added to cultured of chondrocyte, and its effect on the mRNA expression of type II collagen was quantitatively evaluated. Results As a result of treatment with Yukmijihwang-Tang gamibang in vitro for 48 hours, the mRNA expression of type II collagen was up-regulated. In addition, the result of H&E-staining in vivo indicated that chondrocyte-like tissues were formed in repairing injured cartilages after 12 weeks of treatment with Yukmijihwang-Tang gamibang. Conclusions These results indicated that Yukmijihwang-Tang gamibang was effective on the recovery of chondrocyte phenotype, and could be used for cartilage regeneration in arthritic diseases. However, more clinical study of Oriental medical treatment for this case might be also needed.

Keywords

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