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Usefulness of Real-time PCR to Detect Mycobacterium tuberculosis and Nontuberculous Mycobacteria

결핵균과 비결핵성항산균 검출에 Real-time PCR의 유용성

  • Yun, Eun-Young (Department of Internal Medicine, Gyeongsang National University School of Medicine) ;
  • Cho, Su-Hee (Department of Internal Medicine, Gyeongsang National University School of Medicine) ;
  • Go, Se-Il (Department of Internal Medicine, Gyeongsang National University School of Medicine) ;
  • Baek, Jong-Ha (Department of Internal Medicine, Gyeongsang National University School of Medicine) ;
  • Kim, You-Eun (Department of Internal Medicine, Gyeongsang National University School of Medicine) ;
  • Ma, Jeong-Eun (Department of Internal Medicine, Gyeongsang National University School of Medicine) ;
  • Lee, Gi-Dong (Department of Internal Medicine, Gyeongsang National University School of Medicine) ;
  • Cho, Yu-Ji (Department of Internal Medicine, Gyeongsang National University School of Medicine) ;
  • Jeong, Yi-Yeong (Department of Internal Medicine, Gyeongsang National University School of Medicine) ;
  • Kim, Ho-Cheol (Department of Internal Medicine, Gyeongsang National University School of Medicine) ;
  • Lee, Jong-Deok (Department of Internal Medicine, Gyeongsang National University School of Medicine) ;
  • Kim, Sun-Joo (Gyeongsang Institute of Health Sciences, Gyeongsang National University) ;
  • Hwang, Young-Sil (Department of Internal Medicine, Gyeongsang National University School of Medicine)
  • 윤은영 (경상대학교 의학전문대학원 내과학교실) ;
  • 조수희 (경상대학교 의학전문대학원 내과학교실) ;
  • 고세일 (경상대학교 의학전문대학원 내과학교실) ;
  • 백종하 (경상대학교 의학전문대학원 내과학교실) ;
  • 김유은 (경상대학교 의학전문대학원 내과학교실) ;
  • 마정은 (경상대학교 의학전문대학원 내과학교실) ;
  • 이기동 (경상대학교 의학전문대학원 내과학교실) ;
  • 조유지 (경상대학교 의학전문대학원 내과학교실) ;
  • 정이영 (경상대학교 의학전문대학원 내과학교실) ;
  • 김호철 (경상대학교 의학전문대학원 내과학교실) ;
  • 이종덕 (경상대학교 의학전문대학원 내과학교실) ;
  • 김선주 (경상대학교 건강과학연구원) ;
  • 황영실 (경상대학교 의학전문대학원 내과학교실)
  • Received : 2010.06.11
  • Accepted : 2010.08.05
  • Published : 2010.10.30

Abstract

Background: The purpose of this study was to evaluate recently developed real-time polymerase chain reaction (PCR) assay kit to detect Mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM) in respiratory specimens. Methods: We assessed the positive rate of the real-time PCR assay to detect MTB and NTM in 87 culture-positive specimens (37 sputum, 50 bronchial washing), which were performed real-time PCR by using $Real-Q_{TM}$ MTB&NTM Kit from January 2009 to June 2009, at Gyeongsang University Hospital. To compare the efficacy with the TB-PCR assay, we evaluated 63 culture-positive specimens (19 sputum, 44 bronchial washing) for MTB or NTM, which were performed TB-PCR by using ABSOLUTETM MTB II PCR Kit from March 2008 to August 2008. Results: Among 87 specimens tested using real-time PCR, MTB and NTM were cultured in 58 and 29, respectively. The positive rate of real-time PCR assay to detect MTB was 71% (22/31) and 92.6% (25/27) in AFB stain-negative and stain-positive specimens. For NTM, the positive rate of real-time PCR was 11.1% (2/18) and 72.7% (8/11) in AFB stain-negative and stain-positive specimens. Among 63 specimens performed using TB-PCR, MTB and NTM were cultured in 46 and 17, respectively. The positive rate of TB-PCR was 61.7% (21/34) and 100% (12/12) in AFB stain-negative and stain-positive specimens. TB-PCR was negative in all NTM-cultured 17 specimens. Conclusion: TB/NTM real-time PCR assay is useful to differentiate MTB and NTM in AFB stain-positive respiratory specimens and it is as effective in detecting MTB with TB-PCR.

Keywords

References

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