한국응용곤충학회지 (Korean journal of applied entomology)

  • 제49권1호
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  • Pages.43-47
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  • 2010
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  • 1225-0171(pISSN)
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  • 2287-545X(eISSN)
한국응용곤충학회 (Korean Society of Applied Entomology)
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백강균의 분생자병속 형성 조건

Conditions for Formation of Synnemata from Beauveria bassiana

  • 이기만 ((주)마이코플러스) ;
  • 남성희 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 윤철식 ((주)마이코플러스) ;
  • 전지영 ((주)마이코플러스) ;
  • 여주홍 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 이광길 (농촌진흥청 국립농업과학원 농업생물부)
  • 투고 : 2010.02.22
  • 심사 : 2010.03.15
  • 발행 : 2010.03.30
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초록

백강균(Beauveria bassiana)의 분생자병속 형성 조건 구명을 위해 본 실험을 수행하였다. 균은 제주도 설악산 일대에서 치사한 참매미로부터 분리되었다. 분생자병속 형성 유도 배지는 현미와 누에번데기를 원료로 하여 90:10 비율로 조제한 경우가 가장 효과적이었으며, 기타 배지 상에서는 분생자병속 발생이 관찰되지 않았다. 분생자병속은 공기 유입량이 최소일 때 형성이 효과적이었으며, 분생자병속 발생의 최적 온도는 $20^{\circ}C$, 배지 수분함량은 60% 및 조도 300 lux이었다. 또한, 증식에 활용한 종균은 액체배양균이 무성포자보다 분생자병속 형성에 더욱 효과적이었다.

This study was conducted to investigate optimal conditions for formation of synnemata from Beauveria bassiana. The strain of B. bassiana was isolated from a host of Oncotympana fuscata in Halla mountain of Jeju island. The yield of synnemata was the highest at application of brown rice (90%) and silkworm pupae (10%) media. On the other hand, the other media did not result in formation of synnemata. The highest formation of synnemata was achieved at conditions of moderate air inflow and $20^{\circ}C$. The optimal moisture and light intensity for formation of synnemata were 60% and 300 lux, respectively. In addition, inoculation of the liquid spawn resulted in higher yield than that of asexual spore.

키워드

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피인용 문헌

  1. Comparison of scanning electron microscopic structures and nucleotide sequences variation of ITS1, 5.8S ribosomal RNA gene and ITS2 region in three Peruvian entomopathogenic fungal isolates vol.51, pp.2, 2013, https://doi.org/10.7852/jses.2013.51.2.137