The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Effect of Hydrophobicity of Oyster Mushroom on the Severity of Brown Blotch

느타리 갓 표면의 소수성이 세균갈색무늬병 발병도에 미치는 영향

  • Lee, Ki-Young (Department of Agricultural Biology, Chungbuk National University) ;
  • Yang, Seung-Up (Department of Agricultural Biology, Chungbuk National University) ;
  • Han, Jong-Woo (Department of Agricultural Biology, Chungbuk National University) ;
  • Yoon, Kun-Sik (Department of Agricultural Biology, Chungbuk National University) ;
  • Jang, Hoo-Bong (Chungbuk Agricultural Research & Extension services) ;
  • Cha, Jae-Soon (Department of Agricultural Biology, Chungbuk National University)
  • Received : 2010.04.17
  • Accepted : 2010.05.07
  • Published : 2010.06.30
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Abstract

The 2 strains of Pleurotus ostreatus showing very severe and mild symptom of brown blotch were selected, and their F1 hybrid strains were made by mating of their single spore isolates. Hydrophobicity of fruiting body surface and brown blotch severity of the parental and their 11 F1 hybrid strains showing different level of severity of brown blotch were determined. Correlation coefficient (R) between disease severity and hydrophobicity were 0.68 and 0.70 on two independent experiments. Correlation coefficient between disease severity and disease incidence that was determined in the oyster mushroom farm were 0.46 and 0.52 on two independent experiments. When GFP-tagged Pseudomonas tolaasii was monitored with confocal microscope on cap surface of fruiting body, more cells of the pathogen were clustered on the severe strain than the mild strain, which indicates that the bacterial pathogen proliferates more on the severe strain. These results suggest that hydrophobicity of oyster mushroom fruiting body affects disease severity of the brown blotch disease; the longer the bacterial suspension stays on fruiting body surface more severe symptom of the blotch develops.

세균갈색무늬병 병원균의 접종에 의해 심한 병징을 보이는 느타리 균주와 매우 약한 병징을 보이는 균주를 모균주로 선발하고, 각각의 모균주로부터 얻은 단핵균주의 교배를 통해 F1 교잡균주를 얻었다. 세균갈색무늬병 발병도가 다른 11개의 F1균주와 모균주의 자실체 표면의 소수성과 발병도를 조사하였다. 발병도와 소수성 사이의 상관계수는 2번의 독립된 실험에서 각각 0.68과 070이었다. 자실체의 발병도와 버섯재배사에서 조사한 발병율 사이의 상관계수는 각각 046과 0.52이었다. GFP을 발현시킨 Pseudomonas tolaasii을 자실체표면에 접종 후 공초점현미경으로 관찰한 결과 발병도가 낮은 균주의 자실체 표면에서보다 높은 균주의 자실체 표면에서 보다 많은 세균의 집단화가 관찰되었는데, 이 결과는 발병도가 높은 균주의 갓 표면에서 더많은 세균이 증식하였음을 의미한다. 이상의 결과는 느타리 자실체 표면의 소수성이 세균갈색무늬병 발병도에 영향을 미치는 것을 암시한다. 즉 세균현탁액 방울이 갓 표면에 오래 유지되면 더 심한 병징이 형성된다.

Keywords

References

  1. 김우재. 2002. 세균성갈색무늬병에 대한 느타리 품종저항성. 농학석사학위논문. 충북대학교.
  2. 김종완, 김근희, 강희진. 1994. 인공 재배버섯에 질병을 일으키는 Pseudomonas속 병원세균에 관한 연구; 1. 인공 재배버섯의 부패 변성 원인세균에 대하여. 한국식물병리학회지 10:197-210.
  3. 신관철, 전낙범. 1991. 느타리 세균성갈반병의 병원균 분류 동정 및 생물학적 방제. 농시논문집 34:1-10.
  4. 이혁인. 1999. 느타리 세균성갈색무늬병균 Pseudomonas tolaasii의 검출 및 동정을 위한 PCR방법 개발. 농학석사학위논문. 충북대학교.
  5. 조용섭, 박창석, 이순구, 이영근, 임춘근, 차재순, 최용철, 최재을, 허성기, 황인규. 2002. 식물세균병학. 서울대학교출판부. pp. 295-297.
  6. 주영철, 하태문, 이윤혜, 이한범, 전대훈, 최종인, 김정한, 장명준. 2008. 버섯재배 바로알기. pp. 12-164.
  7. 진경식, 김정준, 전창성, 이은종. 1994. 버섯 갈변병균 Pseudomonas tolaasii와 Pseudomonas agarici의 분리동정에 있어서 특성차이. 농업논문집 36:343-348
  8. 차동열, 박정식, 유창현, 김광포, 전창성, 이두원. 1997. 느타리 재배 기술과 재배경영. 농민신문사 p374.
  9. Brodey, C. L., Rainey, P. B., Tester, M. and Johnstone, K. 1991. Bacterial blotch disease of the cultivated mushroom is caused by an ion channel forming lipodepsipeptide toxin. Mol. Plant-Microbe Interaction 1:407-411.
  10. Grewal, S. I. S., Han, B., and Johnstone, K. 1995. Identification and characterization of a locus which regulates multiple functions in Pseudomonas tolaasii, the cause of brown blotch disease of Agaricus bisporus. J. Bacteriol. 177:4658-4668 https://doi.org/10.1128/jb.177.16.4658-4668.1995
  11. Munsch, P., Olivier J., and Houdeau, G. 1991. Experimental control of bacterial blotch by bacteriophages. Science of cultivation edible fungi.Maher(ed) Balkema. Rotterdam. pp. 389-396.
  12. Murata, H., Tsukamoto, T., and Shirata, S. 1998. rtp A, a gene encoding a bacterial two-component sensor kinase, determines pathogenic traits of Pseudomonas tolaasii, the causal agent of brown blotch disease of a cultivated mushroom, Pleurotus ostreatus. Mycoscience 39:261-271. https://doi.org/10.1007/BF02464007
  13. Nair, N. G., and Fahy, P. C. 1973. Toxin production by Pseudomonas tolaasii Paine. Aust. J. biol. Sci. 26:509-512. https://doi.org/10.1071/BI9730509
  14. Nutkins, J. C., Mortishire-Smith, R. J., Packman, L. C., Brodey, C. L., Rainey, P. B., Johnstone, K., and Williams, D. H. 1991. Structure determination of tolaasin, an extracellular lipodepsipeptide produced by the mushroom pathogen Pseudomonas tolaasii Paine. J. Am. Chem. Soc. 113:2621-2627. https://doi.org/10.1021/ja00007a040
  15. Rainey, P. B., Brodey, C. L., and Johnstone, K. 1991. Biological properties and spectrum of activity of tolaasin, a lipodepsipeptide toxin produced by the mushroom pathogen Pseudomonas tolaasii Paine. Physiological and Molecular Plant Pathology 39:57-70. https://doi.org/10.1016/0885-5765(91)90031-C
  16. Rainey, P. B., Brodey, C. L., and Johnstone, K. 1993. Identofication of a gene cluster encoding three high-molecular-weight proteins, which is required for synthesis of tolaasin by the mushroom pathogen Pseudomonas tolaasii. Mol. Microbiol. 8:643-652. https://doi.org/10.1111/j.1365-2958.1993.tb01608.x
  17. Robert, C., Levente, B., Jozsef, K., Colin M. J., and Kornel L. K. 2003. Genes involved in the copper-dependent regulation of soluble methane monooxygenase of Methyloccus capsulatus (Bath): cloning, sequencing and mutational analysis. Microbiology 149:1785-1795. https://doi.org/10.1099/mic.0.26061-0
  18. Shirata A., Sugaya, K., Takasugi, M., and Monde, K. 1995. Isolation and biological activity of toxins produced by a Japanese strain of Pseudomonas tolaasii the pathogen of bacterial rot of cultivated oyster moshroom. Ann. Phytopathol. Soc. Jpn. 61:493-502. https://doi.org/10.3186/jjphytopath.61.493
  19. Soler-Riveas, C., Jolivet, S., Arpin, N., Oliver J. M., and Wichers, H. J. 1999. Biochemical and physiological aspects of brown blotch disease of Agaricus bisporus. FEMS Microbiology Reviews 23:591-614.
  20. Tsuneda, A., Suyama, K., Murakami, S., and Ohira, A. 1995. Occurrence of Pseudomonas tolaasii on fruiting bodies of Lentimula edodes formed Quercus logs. Mycoscience 36:283-288. https://doi.org/10.1007/BF02268603
  21. Wells, J. M., Sapers, G. M., Fett, W. F., Butterfield, J. E., Jones, J. B., Bouzar, H., and Miller, F. C. 1996. Postharvest discoloration of the cultivated mushroom Agaricus bisporus caused by Pseudomonas tolaasii, P. ‘reactans’, and P. ‘gingeri’. Phytopathology 86:1098-1104. https://doi.org/10.1094/Phyto-86-1098