The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Purification of Anti-obesity Lipase Inhibitor from the Fruiting Body of Phellinus linteus

상황버섯 자실체로부터 항비만성 라이페이스 저해물질의 정제

  • Lee, Jong-Kuk (Chungngnam Agricultural Research & Extension Services) ;
  • Song, Jung-Hwa (Dept. of Life Science and Genetic Engineering, Paichai University) ;
  • Lee, Jong-Soo (Dept. of Life Science and Genetic Engineering, Paichai University)
  • Received : 2010.06.09
  • Accepted : 2010.06.12
  • Published : 2010.06.30
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Abstract

For development of anti-obesity nutraceuticals from mushrooms, new anti-obesity lipase inhibitor from Phellinus linteus was purified by systematic solvent extraction, TLC and HPLC and characterized. Methanol extract from P. linteus most effectively inhibited(72.5%) porcine pancreatic lipase and ethyl acetate fraction showed the highest inhibitory activity in the systematic solvent extraction. A lipase inhibitor from the ethyl acetate fraction was purified through following steps including 3 times silica gel chromatography and preparative HPLC. The purified lipase inhibitor was a yellowish geen powder and its $IC_{50}$ value was 175 ng. Its molecular weight by MALDI-TOF-MS was 523.06 Da and showed maximal absorption spectrum at 225.1 nm.

본 연구에서는 버섯으로부터 새로운 항비만 치료(예방) 효과를 갖는 물질을 추출, 정제하여 그 특성을 규명하고, 이를 기능성식품 개발의 소재로 용용하고자 lipase 저해 활성이 높은 상황버섯(Phellinus linteus)의 lipase 저해 물질을 각종 chromatography로 정제 하였다. 먼저 상황버섯(Phellinus linteus) 자실체 추출물에 대한 용매 계통분획을 실시하여 저해 활성이 가장 높은 에틸아세테이트 추출물을 얻었고 이를 3차례의 sillicas gel column chromatography와 분취용 HPLC를 실시하여 $IC_{50}$값이 175 ng의 lipase 저해활성을 가진 연한 연두색 분말의 정제된 lipase 저해 물질을 얻었다. 정제한 lipase 저해물질을 일련의 NMR spectrometry를 실시한 결과 분자량이 523.06 Da으로 추정되었고 225.1 nm에서 최대 흡수 파장을 가진 물질이었다.

Keywords

References

  1. 김영진, 김보혜, 이선이, 김민수, 박찬선, 이문수, 이강현, 김동섭. 2006. 항비만 기능성 식품소재 개발을 위한 생약 추출물의 탐색. 한국응용생화학회지 49:221-226.
  2. 김정상, 임진규, 이정순, 김성희, 임현애, 김정현. 2004. 탄수화물과 지방 소화 억제 소재를 이용한 체중조절용 식품의 개발. 농림부 농림기술과제 보고서.
  3. 김현복. 1999. 뽕잎 아이스크림의 관능적 특성 및 혈당 상승억제효과. 한국잠사학회지 41:140-146.
  4. Hackman, R. M. 1997. 체중조절용식품의 기능성소재건강보조 식품 심포지움.한국식품과학회 16:21-39.
  5. 홍석산, 권석형. 1998. 동아의 생리활성. 식품기술 11:7-10.
  6. Bitou, N., Nimomiya, M., Tsjita, T. and Okuda, H. 1999. Screening of lipase inhibitors from marine algae. Lipids 34:441-445. https://doi.org/10.1007/s11745-999-0383-7
  7. Bray, G. A. 1993. Use and abuse of appetiie suppressant drugs in the treatment of obesity. Annals of Internal Medicine 119:707-713. https://doi.org/10.7326/0003-4819-119-7_Part_2-199310011-00016
  8. Bray, G. A. 1998. Drug treatment of obesity. American J. of Clinical Nutrition 67:1-2. https://doi.org/10.1093/ajcn/67.1.1
  9. Bray, G. A., Atkinson, R. L. and Inous, S. 1995. Pharmacologic treatment of obesity. Obesity Research 3:415S-632S. https://doi.org/10.1002/j.1550-8528.1995.tb00207.x
  10. Bray, G. A., Blackburn, G. L., Ferguson, J. M., Greenway F. L., Jain, A. K., Mendel, C. M., Mendels, J., Ryan, D., Schwartz S. L., Scheinbaum, M. L. and Seation, T. B. 1999. Sibutramine produces dose-related weight loss. Obesity Research 7:189-198. https://doi.org/10.1002/j.1550-8528.1999.tb00701.x
  11. Bray, G. A. and Greenway, F. L. 1999. Current and potential drugs for treatment of obesity. Endocrine Reviews 20:805-875. https://doi.org/10.1210/er.20.6.805
  12. Kim, J. S., Kwon, C. S. and Son, K. H. 2000. Inhibition of ${\alpha}$-glucosidase and amylase by luteolin, a flavonoid. Biosci. Biotechnol. Biochem. 64:2458-2461. https://doi.org/10.1271/bbb.64.2458
  13. Levinson, M. L. 1977. Obesity and health. Prev. Med. 6:172-80. https://doi.org/10.1016/0091-7435(77)90016-0
  14. Lee, J. K., Jang, J. H., Lee, J. T. and Lee, J. S. 2010. Extraction and characteristics of anti-obesity lipase inhibitor from Phellinus linteus. Mycobiol. 38:52-57. https://doi.org/10.4489/MYCO.2010.38.1.052
  15. Shimura, S. W., Tsuzuki, W. S., Kobayashi, S. and Suzuki, T. 1992. Inhibitor effect on lipase activity of extracts from medicinal herbs. Biosci. Biotech. Biochem. 56:1478-1479. https://doi.org/10.1271/bbb.56.1478
  16. Taubes, G. 2000. Weight increases worldwide. Science 280:1368.
  17. Vedula U., Schnitzer, P. R. and Tulp, O. L. 1991. The effect of acarbose on the food intake, weight gain, and adiposity of LA/Ncp rats. Comp. Biochem. Physiol. A. 100:477-482. https://doi.org/10.1016/0300-9629(91)90503-5
  18. Zapf, J., Schoenle, E., Waldvogel, M., Sand, M. and Froesch, E. R. 1981. Effect of trypsin treatment of rat adipocyte on biological effects and binding of insulin and insulin-like growth factors. Eur. J. Biochem. 133:605-609.

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