The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Effect of KGD1 Deletion on Cell Wall Biogenesis in Saccharomyces cerevisiae

Saccharomyces cerevisiae의 KGD1 유전자 결손이 세포벽 생합성에 미치는 영향

  • Kim, Sung-Woo (Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Ahn, Ki-Woong (Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Park, Yun-Hee (Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Park, Hee-Moon (Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
  • 김성우 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 안기웅 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 박윤희 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 박희문 (충남대학교 생명시스템과학대학 미생물.분자생명과학과)
  • Received : 2010.06.02
  • Accepted : 2010.06.17
  • Published : 2010.06.30
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Abstract

KGD1 gene was cloned by functional complementation of defects in $\beta$-1,3-glucan synthase activity of the previously isolated Saccharomyces cerevisiae mutant LP0353, which displays a number of cell wall defects at restrictive temperature. We performed the gene disruption experiment to characterize the function of KGD1 gene, which encodes $\beta$-ketoglutarate dehydrogenase, in cell wall biosynthesis. The disruption of KGD1 showed the decreased growth rate, the increase of chitin synthases activity, alterations in cell wall composition, and increase of susceptibility to cell wall inhibitors such as Calcofluor white and Nikkomycin Z. These results suggested that KGD1 might be involved in cell wall biogenesis, especially the biosynthesis of $\beta$-1,6-glucan and chitin in S. cerevisaie.

KGD1 유전자는 비허용온도에서 세포벽에 결함을 보이는 Saccharomyces cerevisiae LP0353 균주의 베타-1,3-글루칸 합성 효소의 활성을 회복시키는 유전자로 분리되었다. $\alpha$-ketoglutarate dehydrogenase를 암호화하는 KGD1 유전자의 효모의 세포벽 합성과 연관된 기능을 분석하기 위하여 유전자 파괴를 시도하였다. KGD1돌연변이는 생장속도가 감소하고, 키틴 합성 효소들의 활성이 증가하였으며, 세포벽 구성 당류의 함량에 변화를 보였다. 또한 Calcofluor white과 Nikkomycin Z 등과 같은 세포벽 합성 저해물질에 대해 감수성 변화를 나타냈다. 이러한 결과들은 KGD1이 효모의 세포벽 특히 베타-1,6-글루칸과 키틴의 생합성에 영향을 주고 있음을 시사한다.

Keywords

References

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