The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Characteristic of Orchid Mycorrhizal Fungi from Roots of Cypripedium japonicum and C. macranthum

광릉요강꽃과 복주머니란의 뿌리에 감염된 난균근균의 특성

  • Sim, Mi-Yeong (Department of Biology Education, Korea National University of Education) ;
  • Youm, Jae-Young (Department of Biology Education, Korea National University of Education) ;
  • Chung, Jae-Min (Department of Forest Resources Conservation, Korea National Arboretum) ;
  • Lee, Byung-Chun (Department of Forest Resources Conservation, Korea National Arboretum) ;
  • Koo, Chang-Duck (Department of Forestry, Chungbuk National University) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 심미영 (한국교원대학교 생물교육과) ;
  • 염재영 (한국교원대학교 생물교육과) ;
  • 정재민 (국립수목원 산림자원보존과) ;
  • 이병천 (국립수목원 산림자원보존과) ;
  • 구창덕 (충북대학교 산림학과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2010.06.17
  • Accepted : 2010.06.21
  • Published : 2010.06.30
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Abstract

Orchid mycorrhizal fungi(OMF) were examined in roots of the two threatened orchid species, Cypripedium japonicum and C. macranthum. The morphological characteristics of mycorrhizal colonization in the roots of two orchid species were observed. OMF colonized in the roots of two species were identified using molecular analysis. DNA from the root was extracted and amplified internal transcribed spacer(ITS) region using basidiomycete ITS primers, ITS1-OF and ITS4-OF. Four species belonging to Tulasnellaceae in roots of C. japonicum and two species of Tulasnellaceae and one basidiomycetous species was found in roots of C. macranthum.

광릉요강꽃(Cypripedium japonicum)과 복주머니란(C. macranthum)의 뿌리에서 난 균근균이 확인되었다. 두 종의 난 뿌리에서 균근의 형태적인 특성을 관찰하였다. 분자적인 분석을 통하여 두 종의 뿌리로부터 난균근균을 확인하였다. 뿌리에서 DNA를 추출하여, 담자균류 ITS 지역에 특이적인 프라이머인 ITS1-OF와 ITS4-OF를 사용하여 ITS 지역을 증폭한 후 염기서열을 분석하여 두 식물의 뿌리에 공생하는 난균근균을 동정하였다. 광릉요강꽃의 뿌리에는 3종류의 Tulasnellaceae와 1종의 담자균류가 발견되었고, 복주머니란의 뿌리에서는 2종류의 Tulasnellaceae에 속하는 균이발견되었다.

Keywords

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