Mycobiology

The Korean Society of Mycology (한국균학회)
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Optimization of Solid-State Fermentation for Improved Conidia Production of Beauveria bassiana as a Mycoinsecticide

  • Pham, Tuan Anh (Department of Bioscience and Biotechnology, The University of Suwon) ;
  • Kim, Jeong-Jun (Applied Entomology Division, National Academy of Agricultural Science) ;
  • Kim, Keun (Department of Bioscience and Biotechnology, The University of Suwon)
  • Received : 2010.04.30
  • Accepted : 2010.05.11
  • Published : 2010.06.30
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Abstract

The production of conidia of entomopathogenic Beauveria bassiana by solid-state fermentation was studied for the development of a biocontrol agent against aphid Myzus persicae. The optimal conditions for conidia production on polished white rice were 40% moisture content, $25^{\circ}C$ culture temperature, 2-day-old seeding culture grown in 3% corn meal, 2% rice bran, 2% corn steep powder medium, initial conidia concentration of $10^7$ conidia/g in the wet rice, 10% inoculum size, and use of a polyethylene bag as a container. The polyethylene bag containing inoculated rice was hand-shaken every 12 hr during fermentation. Using optimal conditions, the maximum conidia production obtained was 4.05 g conidia/100 g dry rice after 14 days of cultivation, a rate 2.83 times higher than conidia yield of pre-optimization.

Keywords

References

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