DOI QR코드

DOI QR Code

Fruit Body Formation on Silkworm by Cordyceps militaris

  • Hong, In-Pyo (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kang, Pil-Don (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Ki-Young (National Academy of Agricultural Science, Rural Development Administration) ;
  • Nam, Sung-Hee (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Man-Young (National Academy of Agricultural Science, Rural Development Administration) ;
  • Choi, Yong-Soo (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Nam-Suk (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Hye-Kyung (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Kwang-Gill (National Academy of Agricultural Science, Rural Development Administration) ;
  • Humber, Richard A. (USDA-ARS Biological Integrated Pest Management Research Unit, Robert W. Holly Center for Agriculture & Health)
  • 투고 : 2010.03.31
  • 심사 : 2010.04.11
  • 발행 : 2010.06.30

초록

Injection inoculation protocols for fruit body formation of Cordyceps militaris (C. militaris) were investigated to improve the incidence of infection in the silkworm species Bombyx mori (B. mori). Injection, with suspensions of C. militaris hyphal bodies into living silkworm pupae, was used to test for fruit body production. Use of Daeseungjam rather than Baegokjam or Keumokjam varieties of B. mori is thought to be suitable for infection by C. militaris. From mounting, nine-day-old to 11-day-old pupae showed the best incidence of infection with a $100\;{\mu}L$ injection volume. Silkworm pupae injected with a hyphal suspension concentration of more than $2\;{\times}\;10^5$ colony-forming unit (cfu) recorded a greater than 96% incidence of infection. Also, fruit bodies of C. militaris were induced and produced at a light intensity between 500 and 1,000 lx.

키워드

참고문헌

  1. Kobayashi Y. The genus Cordyceps and its allies. Sci Rep Tokyo Bunrika Daigaku Sect B 1941;5:53-260.
  2. Petch T. Cordyceps militaris and Isaria farinosa. Trans Br Mycol Soc 1936; 20:216-224. https://doi.org/10.1016/S0007-1536(36)80014-X
  3. Sung JM. The Insects-born fungus of Korea in color. Seoul:Kyohak Publishing Co Ltd.; 1996. p. 62-72.
  4. Liu JB. Forestry history of Ganzi Tibetan Autonomous Prefecture (Chin.). Chengdu: Sichuan Kexue Jisu Chubanshe; 1994. p. 1-323.
  5. Ying JZ, Mao XL, Ma QM. Zong YC, Wen HA. Icones of medicinal fungi from China. Beijing: Science Press; 1987. p.1-575.
  6. Sung JM, Choi YS, Lee HK, Kim SH, Kim YO, Sung GH. Production of fruiting body using cultures of entomopathogenic fungal species. Kor J Mycol 1999;27:15-9.
  7. Cory JG, Suhadolnik RJ, Resnick B, Rich MA. Incoporation of cordycepin (3'-deoxyadenosine) into ribonucleic acid and deoxyribonucleic acid of human tumor cells. Biochem Biophys Acta 1965;103:646-53.
  8. Furuya T, Hirotani M, Matsuzawa M. $N^6$-(2-hydroxyethyl) adenosine, a biologically active compound from cultured mycelia of Cordyceps and Isaria species. Phytochemistry 1983;22:2509-12. https://doi.org/10.1016/0031-9422(83)80150-2
  9. Ha NG, Kim SY, Kang JH, Kang PD, Sung GB, Hong IP. Biological activities and cultural characteristics of an Entomogenous fungus, Paecilomyces tenuipes (Peck) Samson. Korean J Seric Sci 2005;47:12-7.
  10. Kobayashi Y. Keys to the taxa of the genera Cordyceps and Torrubiella. Trans Mycol Soc Jpn 1982;23:329-64.
  11. Spatafora JW, Blackwell M. Molecular systematics of unitunicate perithecial ascomycetes: the Clavicipitales-Hypocreales connection. Mycologia 1993;85:912-22. https://doi.org/10.2307/3760674
  12. Shanor L. The production of mature perithecia of Cordyceps militaris (Linn.) Link in laboratory culture. J Elisha Mitchell Sci Soc 1936;52:99-104.
  13. Sung JM, Choi YS, Shrestha B, Park YJ. Investigation on artificial fruiting of Cordyceps militaris. Kor J Mycol 2002;30:6-10. https://doi.org/10.4489/KJM.2002.30.1.006

피인용 문헌

  1. The medicinal fungus Cordyceps militaris: research and development vol.11, pp.3, 2012, https://doi.org/10.1007/s11557-012-0825-y
  2. from Multi-Ascospore Isolates and Their Single Ascospore Progeny Strains vol.40, pp.2, 2012, https://doi.org/10.5941/MYCO.2012.40.2.100
  3. Isolated in Korea vol.42, pp.4, 2014, https://doi.org/10.5941/MYCO.2014.42.4.317
  4. Fungus vol.43, pp.1, 2015, https://doi.org/10.5941/MYCO.2015.43.1.37
  5. Cordyceps militaris Treatment Preserves Renal Function in Type 2 Diabetic Nephropathy Mice vol.11, pp.11, 2016, https://doi.org/10.1371/journal.pone.0166342
  6. Entomopathogenicity and Biological Attributes of Himalayan Treasured Fungus Ophiocordyceps sinensis (Yarsagumba) vol.3, pp.1, 2017, https://doi.org/10.3390/jof3010004
  7. Enrichment of cordycepin for cosmeceutical applications: culture systems and strategies vol.103, pp.4, 2019, https://doi.org/10.1007/s00253-019-09623-3