The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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The Effects of Daecheongryong-tang on Transcription Factors and Adipogenic Genes during 3T3-L1 Differentiation

대청룡탕이 지방세포 분화기전에 미치는 영향

  • Lee, Jun-Moon (Dept. of Pediatrics, College of Oriental Medicine, Dong Eui University) ;
  • Cho, Sung-Woo (Dept.of Rehabilitation Medicine, College of Oriental Medicine, Dong Eui University) ;
  • Kang, Kyung-Hwa (Dept.of Oriental Physiology, College of Oriental Medicine, Dong Eui University) ;
  • Lee, Seung-Yeon (Dept. of Pediatrics, College of Oriental Medicine, Dong Eui University) ;
  • Yu, Sun-Ae (Dept. of Pediatrics, College of Oriental Medicine, Dong Eui University)
  • 이준문 (동의대학교 한의과대학 소아과교실) ;
  • 조성우 (동의대학교 한의과대학 재활의학과교실) ;
  • 강경화 (동의대학교 한의과대학 생리학교실) ;
  • 이승연 (동의대학교 한의과대학 소아과교실) ;
  • 유선애 (동의대학교 한의과대학 소아과교실)
  • Received : 2010.11.26
  • Accepted : 2010.12.16
  • Published : 2010.12.31
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Abstract

Objectives: The purpose of this study is to investigate the effects of Daecheongryong-tang (DCRT) on the adipogenesis in 3T3-L1 preadipocytes. Methods: 3T3-L1 preadipocytes were differentiated with adipogenic reagents by incubating for 2 days in the absence or presence of DCRT ranging 0.25 and 2%. The effect of DCRT on adipogenesis was examined by Oil red O staining, and the protein, RNA, and RT-PCR were measured. Results: Our results showed that DCRT decreased the TG content by ORO staining. To elucidate the mechanism of the effects of DCRT on lowering TG content in 3T3-L1 adipocytes, we examined the DCRT modulate expressions of transcription factors to induce adipogenesis and adipogenic genes which is related to the regulation of accumulation of lipids. As a result, the expression of SREBP1, C/$EBP{\beta}$, C/$EBP{\delta}$, C/$EBP{\alpha}$, and $PPAR{\gamma}$ genes, which induce the adipose differentiation and adipose-specific aP2, adipsin, LPL, CD36, TGF-${\beta}$ and adiponectin genes which regulates fat formations, were decreased. In addition, DCRT reduced the expression of iNOS and IL-6 in 3T3-L1 adipocytes, resulting in inflammation. Conclusions: DCRT could regulate transcript factor related to induction of adipose differentiation, inhibit the accumulation of lipids and expression of the adipogenic genes.

Keywords

References

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