Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Identification of Lactobacillus plantarum CIB 001 with Bile Salt Deconjugation Activity from Kimchi

김치로부터 담즙산 분해능이 우수한 Lactobacillus plantarum CIB 001의 분리 및 동정

  • Cha, Sang-Do (Biomaterials Research Center, Cellinbio) ;
  • Kim, Tae-Woon (Institute of Life Science & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Dong-Hee (Biomaterials Research Center, Cellinbio)
  • 차상도 ((주)셀인바이오 생물소재연구소) ;
  • 김태운 (경희대학교 식품공학과) ;
  • 이동희 ((주)셀인바이오 생물소재연구소)
  • Received : 2010.02.18
  • Accepted : 2010.05.05
  • Published : 2010.06.28
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Abstract

This study was carried out to isolate and characterize the Lactobacillus plantarum with bile salt deconjugation activity that was isolated from Kimchi. Some isolates were selected and identified as L. plantarum by 16S rRNA gene sequence and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of whole cell protein patterns. They were assayed to determine their capacities to express bile salt hydrolase (BSH) activity. Among the identified strains, L. plantarum CIB 001 showed the highest level of BSH activity. Then, resistance to gastric acidity and bile condition were analyzed for further characterization. This strain was able to maintain viability for 1h at pH 2.0 and to survive in a MRS (deMan, Rogosa, and Sharpe) broth with 1.0% of bile acids. L. plantarum CIB 001 would potentially be useful in the food industry as probiotics.

Keywords

References

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