Osteogenic Differentiation of Bone Marrow Stem Cell using Bi-phase Alginate Scaffold Including BMP-2

BMP-2를 함유한 2상 알지네이트 담체를 이용한 골수줄기세포의 골분화

  • Lim, Hyun-Ju (Department of Advanced Organic Materials Science and Engineering, Kyungpook National University) ;
  • Kim, Hak-Tae (Department of Plastic & Reconstructive Surgery, School of Medicine, Kyungpook National University) ;
  • Oh, Eun-Jung (Department of Advanced Organic Materials Science and Engineering, Kyungpook National University) ;
  • Kim, Tae-Jung (Department of Plastic & Reconstructive Surgery, School of Medicine, Kyungpook National University) ;
  • Ghim, Han-Do (Department of Advanced Organic Materials Science and Engineering, Kyungpook National University) ;
  • Choi, Jin-Hyun (Department of Advanced Organic Materials Science and Engineering, Kyungpook National University) ;
  • Chung, Ho-Yun (Department of Plastic & Reconstructive Surgery, School of Medicine, Kyungpook National University)
  • 임현주 (경북대학교 기능물질공학과) ;
  • 김학태 (경북대학교 의학전문대학원 성형외과교실) ;
  • 오은정 (경북대학교 기능물질공학과) ;
  • 김태정 (경북대학교 의학전문대학원 성형외과교실) ;
  • 김한도 (경북대학교 기능물질공학과) ;
  • 최진현 (경북대학교 기능물질공학과) ;
  • 정호윤 (경북대학교 의학전문대학원 성형외과교실)
  • Received : 2010.03.08
  • Accepted : 2010.04.07
  • Published : 2010.05.10

Abstract

Purpose: The object of this study is to develop a novel BMP-2 delivery system for continuous osteogenic differentiation and to induce osteogenesis of stem cells using a bi-phase alginate carrier in vitro. Methods: Alginate nanoparticle loaded BMP-2 was prepared by the reverse emulsification-diffusion technique. Physical properties and release profiles of alginate carriers were measured by Instron and ELISA kit, respectively. Cell viability and alkaline phosphate activity of hBMSCs differentiation was also evaluated by MTS and Metra BAP assays, respectively. Results: Optimal concentration for bi-phase alginate carrier was determined as 2 wt% by evaluating mechanical and biological properties, and differentiation of BMSCs for bone regeneration. The 2% bi-phase alginate carrier had the lowest initial and final release ratio. In addition, the 2% bi-phase alginate carrier had a little higher ALP activity than the homogeneous carrier. An improved controlled release profile was obtained by combining alginate hydrogel with lyophilized particles. Conclusion: Bi-phase alginate carrier has many advantages such as biocompatibility and controlled release capability. It is expected to be effective as a scaffold and carrier in bone tissue engineering.

Keywords

References

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