International Journal of Oral Biology

  • 제35권1호
  • /
  • Pages.21-25
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  • 2010
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
권호 표지

Development of Species-Specific PCR Primers for the Detection of Streptococcus sobrinus

  • Kim, Sang-Gon (Department of Human Biology, School of Dentistry, Chosun University) ;
  • Yoo, So-Young (Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Kook, Joong-Ki (Department of Oral Biochemistry, School of Dentistry, Chosun University)
  • 투고 : 2009.12.24
  • 심사 : 2010.01.29
  • 발행 : 2010.03.31
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초록

This study was undertaken to develop species-specific forward and universal reverse PCR primers for the detection of Streptococcus sobrinus. These primers target the variable regions of the 16S ribosomal RNA coding gene (rDNA) and their specificity was tested against 10 strains of S. sobrinus strains and 20 different species of oral bacteria using serial dilutions of the purified genomic DNA of S. sobrinus ATCC $33478^T$. Our data show that species-specific amplicons were obtained from all the S. sobrinus strains tested but not from other species. Both direct and nested PCR could detect as little as 400 pg and 4 fg of genomic DNA from S. sobrinus ATCC $33478^T$, respectively. This result suggests that these PCR primers are highly specific and sensitive and applicable to the detection of S. sobrinus.

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참고문헌

  1. Ashimoto A, Chen C, Bakker I, Slots J. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol Immunol. 1996;11:266-73. https://doi.org/10.1111/j.1399-302X.1996.tb00180.x
  2. Beighton D, Russell RRB, Whiley RA. A simple biochemical for the differentiation of Streptococcus mutans and Streptococcus sobrinus. Caries Res. 1991;25:174-8. https://doi.org/10.1159/000261363
  3. de Soet JJ, van Dalen PJ, Pavicic MJ, de Graaff J. Enumeration of mutans streptococci in clinical samples by using monoclonal antibodies. J Clin Microbiol 1990;28:2467-2472.
  4. Gold OG, Jordan HV, Van Houte J. A selective medium for Streptococcus mutans. Arch Oral Biol. 1973;18:1357-64. https://doi.org/10.1016/0003-9969(73)90109-X
  5. Ida H, Igarashi T, Yamamoto A, Goto N, Sasa R. A DNA probe specific to Streptococcus sobrinus. Oral Microbiol Immunol. 1999;14:233-7. https://doi.org/10.1034/j.1399-302X.1999.140406.x
  6. Igarashi T, Ichikawa K, Yamamoto A, Goto N. Identification of mutans streptococcal species by the PCR products of the dex genes. J Microbiol Methods. 2001;46:99-105. https://doi.org/10.1016/S0167-7012(01)00263-9
  7. Igarashi T, Yamamoto A, Goto N. PCR for detection and identification of Streptococcus sobrinus. J Med Microbiol. 2000;49:1069-74. https://doi.org/10.1099/0022-1317-49-12-1069
  8. Jordan HV: Cultural methods for the identification and quantitation of Streptococcus mutans and lactobacilli in oral samples. Oral Microbiol Immunol 1986;1:23-30. https://doi.org/10.1111/j.1399-302X.1986.tb00312.x
  9. Kawamura Y, Hou XG, Sultana F, Liu S, Yamamoto H, Ezaki T: Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus. Int J Syst Bacteriol 1995;45: 406-8. Erratum in: Int J Sys Bacteriol 1995;45: 882. https://doi.org/10.1099/00207713-45-2-406
  10. Lane DJ. 16S/23S rRNA sequencing, pp 115-75. In Stackebrandt E, Goodfellow M.(eds.), Nucleic acid techniques in bacterial systematics. Wiley, New York, 1991.
  11. Loesche WJ. Role of Streptococcus mutans in human dental decay. Microbiol Rev. 1986;50:353-80.
  12. Oho T, Yamashita Y, Shimazaki Y, Kushiyama M, Koga T. Simple and rapid detection of Streptococcus mutans and Streptococcus sobrinus in human saliva by polymerase chain reaction. Oral Microbiol Immunol. 2000;15:258-62. https://doi.org/10.1034/j.1399-302x.2000.150408.x
  13. Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA, Sahasrabudhe A, Dewhirst FE. Bacterial diversity in human subgingival plaque. J Bacteriol. 2001;183:3770-83. https://doi.org/10.1128/JB.183.12.3770-3783.2001
  14. Poyart C, Quesne G, Coulon S, Berche P, Trieu-Cuot P. Identification of streptococci to species level by sequencing the gene encoding the manganese-dependent superoxide dismutase. J Clin Microbiol. 1998;36:41-7.
  15. Rupf S, Merte K, Eschrich K, Stosser L, Kneist S. Peroxidase reaction as a parameter for discrimination of Streptococcus mutans and Streptococcus sobrinus. Caries Res. 2001;35: 258-64. https://doi.org/10.1159/000047467
  16. Sato T, Hu JP, Ohki K, Yamaura M, Washio J, Matsuyama J, Takahashi N. Identification of mutans streptococci by restriction fragment length polymorphism analysis of polymerase chain reaction-amplified 16S ribosomal RNA genes. Oral Microbiol Immunol. 2003a;18:323-6. https://doi.org/10.1034/j.1399-302X.2003.00095.x
  17. Sato T, Matsuyama J, Kumagai T, Mayanagi G, Yamaura M, Washio J, Takahashi N. Nested PCR for detection of mutans streptococci in dental plaque. Lett Appl Microbiol. 2003b; 37:66-9. https://doi.org/10.1046/j.1472-765X.2003.01359.x
  18. Takao A, Nagamune H, Maeda N. Identification of the anginosus group within the genus Streptococcus using polymerase chain reaction. FEMS Microbiol Lett. 2004;1; 233:83-9.
  19. Tanner A, Maiden MF, Paster BJ, Dewhirst FE. The impact of 16S ribosomal RNA-based phylogeny on the taxonomy of oral bacteria. Periodontol 2000. 1994;5:26-51. https://doi.org/10.1111/j.1600-0757.1994.tb00017.x
  20. Whiley RA, Beighton D. Current classification of the oral streptococci. Oral Microbiol Immunol 1998;13:195-216. https://doi.org/10.1111/j.1399-302X.1998.tb00698.x
  21. Yoo SY, Kim PS, Hwang HK, Lim SH, Kim KW, Choe SJ, Min BM, Kook JK. Identification of non-mutans streptococci organisms in dental plaques recovering on mitis-salivarius bacitracin agar medium. J Microbiol. 2005;43:204-8.