Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
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Stimulation of the Immune Response by Yanggyuksanhwa-tang

소양인 양격산화탕의 면역 활성화 연구

  • Jung, Da-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Ho-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Jin-Ah (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Nam-Hun (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Jun-Kyoung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Huang, Dae-Sun (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
  • 정다영 (한국한의학연구원 한약EBM연구센터) ;
  • 하혜경 (한국한의학연구원 한약EBM연구센터) ;
  • 이호영 (한국한의학연구원 한약EBM연구센터) ;
  • 이진아 (한국한의학연구원 한약EBM연구센터) ;
  • 이남헌 (한국한의학연구원 한약EBM연구센터) ;
  • 이준경 (한국한의학연구원 한약EBM연구센터) ;
  • 황대선 (한국한의학연구원 한약EBM연구센터) ;
  • 신현규 (한국한의학연구원 한약EBM연구센터)
  • Received : 2010.05.17
  • Accepted : 2010.06.25
  • Published : 2010.12.31
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Abstract

1. Objectives Yanggyuksanhwa-tang for Soyangin was applied to investigate the immunological activities on antigen (Ag)-specific or Ag-non-specific immune responses on murine macrophage cell line (RAW 264.7) and ovalbumin/aluminium (OVA/Alum)-immunized mice. 2. Methods This study were carried out in nitric oxide (NO) synthesis on RAW 264.7 cells and cellular proliferation on mouse splenocytes. C57BL/6 mice were immunized intraperitonially with OVA/Alum (100 ${\mu}g$/200 ${\mu}g$) on day 1, 8, and 15. Yanggyuksanhwa-tang was administrated to mice orally for 3 weeks from day 1. On day 22, OVA-, lipopolysaccharide (LPS)-, and concanavalin A (Con A)-stimulated splenocyte proliferation and antibodies (OVA-specific antibodies of the IgG, IgG1, and total IgM classes) in plasma were measured. 3. Results Yanggyuksanhwa-tang significantly enhanced cellular proliferation by LPS and Con A on splenocytes from OVA/Alum-immunized mice (p<.001). Yanggyuksanhwa-tang also significantly enhanced plasma OVA-specific IgG (p<.001), IgG1 (p<.001), and total IgM (p<.01) levels compared with the OVA/Alum group. 4. Conclusions These results suggested that Yanggyuksanhwa-tang for Soyangin could be used as immunopotent.

Keywords

References

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